Experiment description of studies that identified SERF2 in sEVs

1 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for SERF2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ATG16L1 55054 Affinity Capture-MS Homo sapiens 2 WDR46   Affinity Capture-MS Homo sapiens 3 PDZD11 51248 Affinity Capture-MS Homo sapiens 4 CCDC59   Affinity Capture-MS Homo sapiens 5 GTF2F1 2962 Affinity Capture-MS Homo sapiens 6 FAM204A   Affinity Capture-MS Homo sapiens 7 PMPCB 9512 Affinity Capture-MS Homo sapiens 8 DDX46 9879 Affinity Capture-MS Homo sapiens 9 PSMA3 5684 Two-hybrid Homo sapiens 10 CHD3 1107 Two-hybrid Homo sapiens 11 ZAK 51776 Affinity Capture-MS Homo sapiens 12 MAP7 9053 Affinity Capture-MS Homo sapiens 13 RBPMS 11030 Two-hybrid Homo sapiens 14 LEO1 123169 Affinity Capture-MS Homo sapiens 15 ZRANB2 9406 Affinity Capture-MS Homo sapiens 16 SPATS2   Affinity Capture-MS Homo sapiens 17 C9orf78 51759 Affinity Capture-MS Homo sapiens 18 PRPF38B 55119 Affinity Capture-MS Homo sapiens 19 PSME3 10197 Two-hybrid Homo sapiens 20 PDIA4 9601 Cross-Linking-MS (XL-MS) Homo sapiens 21 SIRT7   Affinity Capture-MS Homo sapiens 22 NELFCD 51497 Two-hybrid Homo sapiens 23 GC 2638 Two-hybrid Homo sapiens 24 QKI 9444 Two-hybrid Homo sapiens 25 PAK2 5062 Affinity Capture-MS Homo sapiens 26 RBMXL1   Affinity Capture-MS Homo sapiens 27 ZCCHC17   Affinity Capture-MS Homo sapiens 28 ESF1 51575 Affinity Capture-MS Homo sapiens 29 MDFI   Two-hybrid Homo sapiens 30 DAB1 1600 Two-hybrid Homo sapiens 31 RIOK1 83732 Affinity Capture-MS Homo sapiens 32 NFATC2IP   Affinity Capture-MS Homo sapiens 33 RHOXF2   Two-hybrid Homo sapiens 34 CCDC97   Affinity Capture-MS Homo sapiens 35 CWC25 54883 Affinity Capture-MS Homo sapiens 36 RABL6 55684 Affinity Capture-MS Homo sapiens 37 THAP1   Two-hybrid Homo sapiens 38 RP9   Affinity Capture-MS Homo sapiens 39 SP4   Two-hybrid Homo sapiens 40 MTDH 92140 Affinity Capture-MS Homo sapiens 41 RTF1 23168 Affinity Capture-MS Homo sapiens 42 GPKOW   Affinity Capture-MS Homo sapiens 43 SPAST 6683 Affinity Capture-MS Homo sapiens 44 IWS1   Affinity Capture-MS Homo sapiens 45 DHX16 8449 Affinity Capture-MS Homo sapiens 46 TJP2 9414 Affinity Capture-MS Homo sapiens 47 GTPBP4 23560 Affinity Capture-MS Homo sapiens 48 DHX38 9785 Affinity Capture-MS Homo sapiens 49 GTF2F2 2963 Affinity Capture-MS Homo sapiens 50 APOA2 336 Affinity Capture-MS Homo sapiens 51 MAP9 79884 Affinity Capture-MS Homo sapiens 52 PRRC2A 7916 Affinity Capture-MS Homo sapiens 53 RRP36 88745 Affinity Capture-MS Homo sapiens 54 KRTAP10-3   Two-hybrid Homo sapiens 55 LENG1   Affinity Capture-MS Homo sapiens 56 C12orf43   Affinity Capture-MS Homo sapiens 57 NSRP1   Affinity Capture-MS Homo sapiens 58 LDLR 3949 Negative Genetic Homo sapiens 59 SUPV3L1 6832 Affinity Capture-MS Homo sapiens 60 ERC1 23085 Affinity Capture-MS Homo sapiens 61 NOB1   Affinity Capture-MS Homo sapiens 62 UTP11L   Affinity Capture-MS Homo sapiens 63 ZGPAT   Affinity Capture-MS Homo sapiens 64 BOLL   Two-hybrid Homo sapiens 65 ARGLU1   Affinity Capture-MS Homo sapiens 66 CTR9 9646 Affinity Capture-MS Homo sapiens 67 EIF5B 9669 Affinity Capture-MS Homo sapiens 68 ZNHIT3   Affinity Capture-MS Homo sapiens 69 RIOK2 55781 Affinity Capture-MS Homo sapiens 70 UPF2 26019 Two-hybrid Homo sapiens 71 DDX52   Affinity Capture-MS Homo sapiens 72 DNTTIP2   Affinity Capture-MS Homo sapiens 73 CEBPA   Protein-peptide Homo sapiens 74 KIF20B   Affinity Capture-MS Homo sapiens 75 SNRNP27   Affinity Capture-MS Homo sapiens 76 RRP7A 27341 Affinity Capture-MS Homo sapiens View the network

Pathways in which SERF2 is involved

No pathways found