Experiment description of studies that identified TPPP in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for TPPP

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 2 MAN2C1 4123 Affinity Capture-MS Homo sapiens 3 SNCA 6622 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 4 PCTP   Affinity Capture-MS Homo sapiens 5 FADD 8772 Affinity Capture-MS Homo sapiens 6 TUBB 203068 Reconstituted Complex Homo sapiens 7 SPTBN4 57731 Affinity Capture-MS Homo sapiens 8 CDK11B 984 Two-hybrid Homo sapiens 9 ROCK1 6093 Biochemical Activity Homo sapiens 10 SHOC2 8036 Co-fractionation Homo sapiens 11 LIN28A   Affinity Capture-MS Homo sapiens 12 HDAC6 10013 Reconstituted Complex Homo sapiens 13 C2orf27B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 CHST5   Affinity Capture-MS Homo sapiens 15 GRPEL1 80273 Affinity Capture-MS Homo sapiens 16 SEMG2 6407 Affinity Capture-MS Homo sapiens 17 APP 351 Reconstituted Complex Homo sapiens 18 SEMG1 6406 Affinity Capture-MS Homo sapiens 19 DCAF10   Affinity Capture-MS Homo sapiens 20 GNB2 2783 Affinity Capture-MS Homo sapiens 21 KLHL24   Affinity Capture-MS Homo sapiens 22 ACPP 55 Affinity Capture-MS Homo sapiens 23 HIST1H1A 3024 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 ZNF114 163071 Two-hybrid Homo sapiens 25 IFT74 80173 Co-fractionation Homo sapiens View the network

Pathways in which TPPP is involved

No pathways found