Gene description for AKR1B10
Gene name aldo-keto reductase family 1, member B10 (aldose reductase)
Gene symbol AKR1B10
Other names/aliases AKR1B11
AKR1B12
ALDRLn
ARL-1
ARL1
HIS
HSI
Species Homo sapiens
 Database cross references - AKR1B10
ExoCarta ExoCarta_57016
Vesiclepedia VP_57016
Entrez Gene 57016
HGNC 382
MIM 604707
UniProt O60218  
 AKR1B10 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for AKR1B10
Molecular Function
    retinal dehydrogenase activity GO:0001758 IDA
    aldose reductase (NADPH) activity GO:0004032 IBA
    aldo-keto reductase (NADPH) activity GO:0004033 TAS
    protein binding GO:0005515 IPI
    alcohol dehydrogenase (NADP+) activity GO:0008106 IDA
    geranylgeranyl reductase activity GO:0045550 IDA
    allyl-alcohol dehydrogenase activity GO:0047655 IEA
    indanol dehydrogenase activity GO:0047718 IDA
    all-trans-retinol dehydrogenase (NADP+) activity GO:0052650 IDA
Biological Process
    retinoid metabolic process GO:0001523 IDA
    retinoid metabolic process GO:0001523 TAS
    farnesol catabolic process GO:0016488 IDA
    retinol metabolic process GO:0042572 IEA
    daunorubicin metabolic process GO:0044597 IMP
    doxorubicin metabolic process GO:0044598 IMP
    cellular detoxification of aldehyde GO:0110095 IDA
Subcellular Localization
    extracellular region GO:0005576 IDA
    lysosome GO:0005764 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified AKR1B10 in sEVs
1
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for AKR1B10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FNDC5  
Affinity Capture-MS Homo sapiens
2 AKR1B15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 COX17  
Co-fractionation Homo sapiens
5 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
6 SLC25A6 293
Affinity Capture-MS Homo sapiens
7 C18orf21  
Affinity Capture-MS Homo sapiens
8 AGPAT1 10554
Affinity Capture-MS Homo sapiens
9 INPPL1 3636
Affinity Capture-MS Homo sapiens
10 DDX19B 11269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TSNAX 7257
Affinity Capture-MS Homo sapiens
13 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 C1orf35  
Affinity Capture-MS Homo sapiens
15 TERF2IP 54386
Two-hybrid Homo sapiens
16 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
17 SOX2  
Affinity Capture-MS Homo sapiens
18 CERS3  
Affinity Capture-MS Homo sapiens
19 PCBP3 54039
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 POTEF 728378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 AKR1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SRRT 51593
Affinity Capture-MS Homo sapiens
23 ACACA 31
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 SSUH2  
Affinity Capture-MS Homo sapiens
25 PGK1 5230
Co-fractionation Homo sapiens
26 VCP 7415
Affinity Capture-MS Homo sapiens
27 PIGT 51604
Affinity Capture-MS Homo sapiens
28 GOT1 2805
Affinity Capture-MS Homo sapiens
29 NOS2  
Affinity Capture-MS Homo sapiens
30 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 Jup 16480
Affinity Capture-MS Mus musculus
32 OR2A4 79541
Affinity Capture-MS Homo sapiens
33 CCR1  
Affinity Capture-MS Homo sapiens
34 HINT1 3094
Co-fractionation Homo sapiens
35 STX17 55014
Affinity Capture-MS Homo sapiens
36 Slain1  
Affinity Capture-MS Mus musculus
37 Nedd1  
Affinity Capture-MS Mus musculus
38 PHF11 51131
Affinity Capture-MS Homo sapiens
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