Gene description for ARHGAP29
Gene name Rho GTPase activating protein 29
Gene symbol ARHGAP29
Other names/aliases PARG1
Species Homo sapiens
 Database cross references - ARHGAP29
ExoCarta ExoCarta_9411
Vesiclepedia VP_9411
Entrez Gene 9411
HGNC 30207
MIM 610496
UniProt Q52LW3  
 ARHGAP29 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGAP29
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    PDZ domain binding GO:0030165 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    Rho protein signal transduction GO:0007266 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    negative regulation of small GTPase mediated signal transduction GO:0051058 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified ARHGAP29 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGAP29
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CBS 875
Affinity Capture-MS Homo sapiens
2 HERC1 8925
Affinity Capture-MS Homo sapiens
3 CASP14 23581
Affinity Capture-MS Homo sapiens
4 ATPIF1 93974
Affinity Capture-MS Homo sapiens
5 CPSF1 29894
Affinity Capture-MS Homo sapiens
6 IPO5 3843
Affinity Capture-MS Homo sapiens
7 PRPF4B 8899
Affinity Capture-MS Homo sapiens
8 MRPS17 51373
Affinity Capture-MS Homo sapiens
9 LIN7C 55327
Affinity Capture-MS Homo sapiens
10 HSPA8 3312
Cross-Linking-MS (XL-MS) Homo sapiens
11 XPO1 7514
Affinity Capture-MS Homo sapiens
12 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MAGEA11  
Two-hybrid Homo sapiens
14 PCMT1 5110
Affinity Capture-MS Homo sapiens
15 PFN1 5216
Proximity Label-MS Homo sapiens
16 MCM5 4174
Affinity Capture-MS Homo sapiens
17 PARD3 56288
Proximity Label-MS Homo sapiens
18 RIF1  
Affinity Capture-MS Homo sapiens
19 HRAS 3265
Reconstituted Complex Homo sapiens
20 YWHAZ 7534
Affinity Capture-MS Homo sapiens
21 EGFR 1956
Affinity Capture-MS Homo sapiens
22 METTL21B  
Affinity Capture-MS Homo sapiens
23 S100A9 6280
Affinity Capture-MS Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 ANKRD28 23243
Co-fractionation Homo sapiens
26 TRIM54  
Two-hybrid Homo sapiens
27 CAMK2G 818
Affinity Capture-MS Homo sapiens
28 TRIM37  
Proximity Label-MS Homo sapiens
29 KDM1A 23028
Two-hybrid Homo sapiens
30 CDH1 999
Proximity Label-MS Homo sapiens
31 MLLT4 4301
Proximity Label-MS Homo sapiens
32 PTPN13 5783
Reconstituted Complex Homo sapiens
33 CEP135  
Proximity Label-MS Homo sapiens
34 CSNK2A2 1459
Co-fractionation Homo sapiens
35 GOLGA1  
Proximity Label-MS Homo sapiens
36 DTX2 113878
Proximity Label-MS Homo sapiens
37 C14orf166 51637
Affinity Capture-MS Homo sapiens
38 FIP1L1 81608
Affinity Capture-MS Homo sapiens
39 BICD1 636
Proximity Label-MS Homo sapiens
40 GGA2 23062
Proximity Label-MS Homo sapiens
41 CAMK2D 817
Affinity Capture-MS Homo sapiens
42 RHOB 388
Proximity Label-MS Homo sapiens
43 YWHAG 7532
Affinity Capture-MS Homo sapiens
44 CKAP4 10970
Affinity Capture-MS Homo sapiens
45 SIRT1  
Affinity Capture-MS Homo sapiens
46 RHOA 387
FRET Homo sapiens
47 GPR183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FARSA 2193
Affinity Capture-MS Homo sapiens
49 CCNT1  
Affinity Capture-MS Homo sapiens
50 CACYBP 27101
Affinity Capture-MS Homo sapiens
51 EEF1E1 9521
Affinity Capture-MS Homo sapiens
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