Gene description for CD81
Gene name CD81 molecule
Gene symbol CD81
Other names/aliases CVID6
S5.7
TAPA1
TSPAN28
Species Homo sapiens
 Database cross references - CD81
ExoCarta ExoCarta_975
Vesiclepedia VP_975
Entrez Gene 975
HGNC 1701
MIM 186845
UniProt P60033  
 CD81 identified in sEVs derived from the following tissue/cell type
Ascites 21601258    
B cells 12519789    
B cells 9685355    
B cells 17641064    
B cells 20458337    
B cells 20458337    
B cells 20458337    
B cells 21505438    
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 36517516    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 30918259    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 32848136    
Breast cancer cells 35918900    
Breast milk 17641064    
Breast milk 17641064    
Breast milk 21235781    
Cervical cancer cells 34282141    
Cervical cancer cells 34282141    
Cervical cancer cells 34282141    
Cervical cancer cells 34282141    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Colorectal cancer cells 22740476    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 33368224    
Colorectal cancer cells 29472616    
Colorectal cancer cells 29472616    
Colorectal cancer cells 29472616    
Colorectal cancer cells 29472616    
Colorectal cancer cells 35443745    
Colorectal cancer cells 35443745    
Colorectal cancer cells 34887515    
Dendritic cells 21505438    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 30918259    
Embryonic kidney cells 34545097    
Embryonic kidney cells 34545097    
Embryonic kidney cells 34545097    
Faeces 33431899    
Faeces 33431899    
Fibroblasts 29438363    
Fibroblasts 35917363    
Fibroblasts 35917363    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Fibroblasts 36266345    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Glioblastoma 34108659    
Glioblastoma 34108659    
Hepatocellular carcinoma cells 26054723    
Intestinal epithelial cells 33368224    
Liver cancer cells 29335551    
Liver cancer cells 29335551    
Liver cancer cells 29335551    
Liver cancer cells 29335551    
Liver cancer cells 32082513    
Liver cancer cells 32082513    
Liver cancer cells 32082513    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Liver cancer cells 36631249    
Lung cancer cells 32132711    
Lung cancer cells 32132711    
Lymphoma cells 34108659    
Malignant ascites 21601258    
Malignant pleural effusions 12147373    
Mammary cancer-associated fibroblasts 34108659    
Mammary epithelial cells 30918259    
Mast cells 15908444    
Melanoma cells 19801663    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 30918259    
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 25669974    
Mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Monocytes 31489147    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Plasma 15908444    
Plasma 21235781    
Plasma 36455873    
Plasma 36455873    
Plasma 36631249    
Plasma 36631249    
Pluripotent stem cells 34108659    
Posterior longitudinal ligament 35513391    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25857301    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
Saliva 21235781    
Serum 36517516    
Squamous carcinoma cells 20124223    
T cells 21505438    
T lymphocytes 34108659    
Tabecular meshwork cells 21362503    
Thymus 23844026    
Trophoblast cells 21276792    
Urine 15326289    
Urine 19056867    
Urine 20224111    
Urine 20224111    
Urine 33432243    
Urine 33432243    
Urine 33432243    
Urine 33432243    
Urine 33432243    
Urine 33432243    
 Gene ontology annotations for CD81
Molecular Function
    virus receptor activity GO:0001618 IMP
    integrin binding GO:0005178 IDA
    protein binding GO:0005515 IPI
    cholesterol binding GO:0015485 IDA
    MHC class II protein complex binding GO:0023026 HDA
    MHC class II protein binding GO:0042289 IDA
    transferrin receptor binding GO:1990459 IPI
Biological Process
    immunological synapse formation GO:0001771 IMP
    humoral immune response mediated by circulating immunoglobulin GO:0002455 IMP
    positive regulation of inflammatory response to antigenic stimulus GO:0002863 ISS
    myoblast fusion involved in skeletal muscle regeneration GO:0014905 ISS
    positive regulation of B cell proliferation GO:0030890 IDA
    receptor internalization GO:0031623 ISS
    regulation of protein stability GO:0031647 IMP
    macrophage fusion GO:0034238 IDA
    CD4-positive, alpha-beta T cell costimulation GO:0035783 IDA
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    symbiont entry into host cell GO:0046718 IEA
    positive regulation of B cell receptor signaling pathway GO:0050861 IMP
    positive regulation of B cell receptor signaling pathway GO:0050861 ISS
    positive regulation of T cell receptor signaling pathway GO:0050862 IMP
    protein localization to lysosome GO:0061462 IMP
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 IMP
    cellular response to low-density lipoprotein particle stimulus GO:0071404 ISS
    protein localization to plasma membrane GO:0072659 IDA
    protein localization to plasma membrane GO:0072659 IMP
    osteoclast fusion GO:0072675 ISS
    positive regulation of receptor clustering GO:1903911 IDA
    positive regulation of protein catabolic process in the vacuole GO:1904352 IMP
    regulation of macrophage migration GO:1905521 ISS
    positive regulation of adaptive immune memory response GO:1905676 IMP
    positive regulation of T-helper 2 cell cytokine production GO:2000553 IDA
    positive regulation of CD4-positive, alpha-beta T cell proliferation GO:2000563 IDA
    positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell GO:2001190 IDA
Subcellular Localization
    immunological synapse GO:0001772 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 HDA
    basal plasma membrane GO:0009925 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IDA
    vesicle GO:0031982 IDA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
    tetraspanin-enriched microdomain GO:0097197 IDA
 Experiment description of studies that identified CD81 in sEVs
1
Experiment ID 110
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSP70|CD81|CD9|HLA-DR|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Ascites
Sample name Ascites - Liver cirrhosis
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.04-1.10 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
2
Experiment ID 11
MISEV standards
✘ Biophysical techniques
✔
HSC70|HSP90|MHCII|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12519789    
Organism Homo sapiens
Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors "Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W"
Journal name JBC
Publication year 2003
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC
3
Experiment ID 16
MISEV standards
✔
EM
Biophysical techniques
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 9685355    
Organism Homo sapiens
Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors "Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ"
Journal name JBC
Publication year 1998
Sample B cells
Sample name RN (HLA-DR15+)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.12-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Immunoelectron Microscopy
4
Experiment ID 47
MISEV standards
✘ Biophysical techniques
✔
CD81|CD86
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample B cells
Sample name B cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
5
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
6
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
7
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
8
Experiment ID 116
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21505438    
Organism Homo sapiens
Experiment description Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors "Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F."
Journal name NATCOM
Publication year 2011
Sample B cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Mass spectrometry
Microarray
9
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
10
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 458
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|TSG101
Enriched markers
✔
CALR
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36517516    
Organism Homo sapiens
Experiment description Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes
Authors "Zhao D, Wu K, Sharma S, Xing F, Wu SY, Tyagi A, Deshpande R, Singh R, Wabitsch M, Mo YY, Watabe K."
Journal name Nat Commun
Publication year 2022
Sample Breast cancer cells
Sample name HCC1806
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
qPCR
20
Experiment ID 920
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 921
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 924
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 927
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|SDCBP|CD81|RAB35|LAMP2|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 943
MISEV standards
✘ Biophysical techniques
✔
CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Breast cancer cells
Sample name MDA-MB-231-luci (+)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 1181
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 1182
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
27
Experiment ID 1184
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 5
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
28
Experiment ID 1185
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 1186
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 7
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
30
Experiment ID 1187
MISEV standards
✘ Biophysical techniques
✔
FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 8
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
31
Experiment ID 1193
MISEV standards
✘ Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
32
Experiment ID 1197
MISEV standards
✘ Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
33
Experiment ID 1198
MISEV standards
✘ Biophysical techniques
✔
TSG101|CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
34
Experiment ID 1199
MISEV standards
✘ Biophysical techniques
✔
TSG101|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
35
Experiment ID 1200
MISEV standards
✘ Biophysical techniques
✔
TSG101|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32848136    
Organism Homo sapiens
Experiment description ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells
Authors "Fuentes P, Sesé M, Guijarro PJ, Emperador M, Sánchez-Redondo S, Peinado H, Hümmer S, Ramón Y Cajal S."
Journal name Nat Commun
Publication year 2020
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
36
Experiment ID 1333
MISEV standards
Biophysical techniques
✔
CD81|EPCAM|CD9
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
Western blotting
37
Experiment ID 46
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
FACS
Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
38
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
FACS
Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
39
Experiment ID 124
MISEV standards
✔
IEM|EM
Biophysical techniques
✔
HSC70|CD63|CD9|CD81
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21235781    
Organism Homo sapiens
Experiment description "Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages."
Authors "Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H."
Journal name JTM
Publication year 2011
Sample Breast milk
Sample name Breast milk - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
40
Experiment ID 1325
MISEV standards
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
41
Experiment ID 1328
MISEV standards
Biophysical techniques
✔
CD63|SDCBP|CD81|CD9
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
42
Experiment ID 1329
MISEV standards
Biophysical techniques
✔
CD63|SDCBP|LAMP1|CD81|CD9
Enriched markers
✔
CANX
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
43
Experiment ID 1330
MISEV standards
Biophysical techniques
✔
CD63|SDCBP|CD81|CD9
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34282141    
Organism Homo sapiens
Experiment description Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
Authors "Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C."
Journal name Nat Commun
Publication year 2021
Sample Cervical cancer cells
Sample name HeLa - 200K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
44
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
45
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
46
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
47
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
48
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
49
Experiment ID 1265
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
50
Experiment ID 1266
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW480 - Oxaliplatin-resistant
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
51
Experiment ID 1267
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW620
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
52
Experiment ID 1268
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW620 - Oxaliplatin-resistant
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
53
Experiment ID 1269
MISEV standards
Biophysical techniques
✔
CD63|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name COAD - Fraction 4
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.05 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
54
Experiment ID 1270
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name COAD - Fraction 5
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.06 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
55
Experiment ID 1271
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name COAD - Fraction 6
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.08 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
56
Experiment ID 1272
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name COAD - Fraction 7
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
57
Experiment ID 1273
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name COAD - Fraction 8
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
58
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
59
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
60
Experiment ID 201
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22740476    
Organism Homo sapiens
Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors "Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ."
Journal name Electrophoresis
Publication year 2012
Sample Colorectal cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
61
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
62
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
63
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
64
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
65
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
66
Experiment ID 314
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33368224    
Organism Homo sapiens
Experiment description Colorectal cancer prompted adipose tissue browning and cancer cachexia through transferring exosomal miR-146b-5p
Authors "Di W, Zhang W, Zhu B, Li X, Tang Q, Zhou Y. "
Journal name J Cell Physiol
Publication year 2020
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study Western blotting
RT-PCT
67
Experiment ID 336
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|EPCAM|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 29472616    
Organism Homo sapiens
Experiment description Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246
Authors "Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC."
Journal name Nat Commun
Publication year 2018
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
68
Experiment ID 337
MISEV standards
✘ Biophysical techniques
✔
CD9|CD81|EPCAM|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 29472616    
Organism Homo sapiens
Experiment description Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246
Authors "Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC."
Journal name Nat Commun
Publication year 2018
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
69
Experiment ID 342
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|EPCAM
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 29472616    
Organism Homo sapiens
Experiment description Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246
Authors "Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC."
Journal name Nat Commun
Publication year 2018
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Mass spectrometry
miR-chip array
70
Experiment ID 343
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|EPCAM
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 29472616    
Organism Homo sapiens
Experiment description Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246
Authors "Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC."
Journal name Nat Commun
Publication year 2018
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Mass spectrometry
miR-chip array
71
Experiment ID 763
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35443745    
Organism Homo sapiens
Experiment description Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts
Authors "Wang D, Wang X, Song Y, Si M, Sun Y, Liu X, Cui S, Qu X, Yu X."
Journal name Cell Death Dis
Publication year 2022
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Ultracentrifugation
ExoQuick Kit
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RNA sequencing
RT-qPCR
72
Experiment ID 764
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35443745    
Organism Homo sapiens
Experiment description Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts
Authors "Wang D, Wang X, Song Y, Si M, Sun Y, Liu X, Cui S, Qu X, Yu X."
Journal name Cell Death Dis
Publication year 2022
Sample Colorectal cancer cells
Sample name HCT116
Isolation/purification methods Ultracentrifugation
ExoQuick Kit
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RNA sequencing
RT-qPCR
73
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
74
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
75
Experiment ID 117
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21505438    
Organism Homo sapiens
Experiment description Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors "Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F."
Journal name NATCOM
Publication year 2011
Sample Dendritic cells
Sample name Dendritic cells
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Mass spectrometry
Microarray
76
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
77
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
78
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
79
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
80
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
81
Experiment ID 923
MISEV standards
✘ Biophysical techniques
✔
CD9|CD81|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
82
Experiment ID 1145
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34545097    
Organism Homo sapiens
Experiment description Exosome-mediated stable epigenetic repression of HIV-1
Authors "Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV."
Journal name Nat Commun
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
83
Experiment ID 1146
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34545097    
Organism Homo sapiens
Experiment description Exosome-mediated stable epigenetic repression of HIV-1
Authors "Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV."
Journal name Nat Commun
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study Western blotting
RT-qPCR
84
Experiment ID 1147
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD81|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34545097    
Organism Homo sapiens
Experiment description Exosome-mediated stable epigenetic repression of HIV-1
Authors "Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV."
Journal name Nat Commun
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study Western blotting
RT-qPCR
85
Experiment ID 305
MISEV standards
✘ Biophysical techniques
✔
CD63|CD81|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Homo sapiens
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Healthy volunteers
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
HPLC
86
Experiment ID 306
MISEV standards
✘ Biophysical techniques
✔
CD63|CD81|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33431899    
Organism Homo sapiens
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Type 2 diabetes patients
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
HPLC
87
Experiment ID 1088
MISEV standards
✘ Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 29438363    
Organism Homo sapiens
Experiment description CD9-positive exosomes from cancer-associated fibroblasts stimulate the migration ability of scirrhous-type gastric cancer cells
Authors "Miki Y, Yashiro M, Okuno T, Kitayama K, Masuda G, Hirakawa K, Ohira M."
Journal name Br J Cancer
Publication year 2018
Sample Fibroblasts
Sample name NF65
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
88
Experiment ID 1337
MISEV standards
Biophysical techniques
✔
TSG101|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35917363    
Organism Homo sapiens
Experiment description CD9 mediates the uptake of extracellular vesicles from cancer-associated fibroblasts that promote pancreatic cancer cell aggressiveness
Authors "Nigri J, Leca J, Tubiana SS, Finetti P, Guillaumond F, Martinez S, Lac S, Iovanna JL, Audebert S, Camoin L, Vasseur S, Bertucci F, Tomasini R."
Journal name Sci Signal
Publication year 2022
Sample Fibroblasts
Sample name CAF - Pancreatic ductal adenocarcinoma
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
89
Experiment ID 1338
MISEV standards
Biophysical techniques
✔
TSG101|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35917363    
Organism Homo sapiens
Experiment description CD9 mediates the uptake of extracellular vesicles from cancer-associated fibroblasts that promote pancreatic cancer cell aggressiveness
Authors "Nigri J, Leca J, Tubiana SS, Finetti P, Guillaumond F, Martinez S, Lac S, Iovanna JL, Audebert S, Camoin L, Vasseur S, Bertucci F, Tomasini R."
Journal name Sci Signal
Publication year 2022
Sample Fibroblasts
Sample name CAF - Pancreatic ductal adenocarcinoma
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
90
Experiment ID 1348
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
91
Experiment ID 1349
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
92
Experiment ID 1350
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
93
Experiment ID 1351
MISEV standards
Biophysical techniques
✔
FLOT1|CD9|CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
94
Experiment ID 1352
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
95
Experiment ID 1353
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
96
Experiment ID 1354
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
97
Experiment ID 1355
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
98
Experiment ID 1356
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
99
Experiment ID 1357
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
100
Experiment ID 1358
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
101
Experiment ID 1359
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
102
Experiment ID 1360
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36266345    
Organism Homo sapiens
Experiment description Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression
Authors "Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J."
Journal name Nat Commun
Publication year 2022
Sample Fibroblasts
Sample name MRC-5
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
103
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
104
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
105
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
106
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
107
Experiment ID 401
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63|CD81
Enriched markers
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Glioblastoma
Sample name U87
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Flow cytometry
108
Experiment ID 404
MISEV standards
✘ Biophysical techniques
✔
CD81
Enriched markers
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Glioblastoma
Sample name U87
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Flow cytometry
109
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
110
Experiment ID 313
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33368224    
Organism Homo sapiens
Experiment description Colorectal cancer prompted adipose tissue browning and cancer cachexia through transferring exosomal miR-146b-5p
Authors "Di W, Zhang W, Zhu B, Li X, Tang Q, Zhou Y. "
Journal name J Cell Physiol
Publication year 2020
Sample Intestinal epithelial cells
Sample name FHs 74 Int
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study Western blotting
RT-PCT
111
Experiment ID 691
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|CD63|TSG101|CD9|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 29335551    
Organism Homo sapiens
Experiment description Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer
Authors "Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H."
Journal name Nat Commun
Publication year 2018
Sample Liver cancer cells
Sample name CSQT-2
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Microarray
qRT-PCR
112
Experiment ID 692
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|CD63|TSG101|CD9|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 29335551    
Organism Homo sapiens
Experiment description Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer
Authors "Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H."
Journal name Nat Commun
Publication year 2018
Sample Liver cancer cells
Sample name HCC-LM3
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Microarray
qRT-PCR
113
Experiment ID 693
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|CD63|TSG101|CD9|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 29335551    
Organism Homo sapiens
Experiment description Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer
Authors "Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H."
Journal name Nat Commun
Publication year 2018
Sample Liver cancer cells
Sample name HepG2
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Microarray
qRT-PCR
114
Experiment ID 694
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|CD63|TSG101|CD9|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 29335551    
Organism Homo sapiens
Experiment description Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer
Authors "Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H."
Journal name Nat Commun
Publication year 2018
Sample Liver cancer cells
Sample name MHCC-97L
Isolation/purification methods Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Microarray
qRT-PCR
115
Experiment ID 1308
MISEV standards
Biophysical techniques
✔
TSG101|CD63|CD81|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32082513    
Organism Homo sapiens
Experiment description Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles
Authors "Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G."
Journal name J Extracell Vesicles
Publication year 2020
Sample Liver cancer cells
Sample name Huh-7 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
116
Experiment ID 1309
MISEV standards
Biophysical techniques
✔
TSG101|CD63|CD81|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32082513    
Organism Homo sapiens
Experiment description Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles
Authors "Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G."
Journal name J Extracell Vesicles
Publication year 2020
Sample Liver cancer cells
Sample name Huh-7 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
117
Experiment ID 1320
MISEV standards
Biophysical techniques
✔
CD81|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 32082513    
Organism Homo sapiens
Experiment description Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles
Authors "Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G."
Journal name J Extracell Vesicles
Publication year 2020
Sample Liver cancer cells
Sample name Huh-7
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
118
Experiment ID 1371
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
119
Experiment ID 1372
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name PLC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
120
Experiment ID 1373
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
121
Experiment ID 1374
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
122
Experiment ID 1375
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
123
Experiment ID 1376
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
124
Experiment ID 1377
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
125
Experiment ID 1378
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
126
Experiment ID 1381
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
127
Experiment ID 1382
MISEV standards
Biophysical techniques
✔
CD63|CD81|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
128
Experiment ID 1383
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
129
Experiment ID 1384
MISEV standards
Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Liver cancer cells
Sample name 97H
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
130
Experiment ID 328
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 32132711    
Organism Homo sapiens
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Lung cancer cells
Sample name A549
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Flow cytometry
131
Experiment ID 329
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 32132711    
Organism Homo sapiens
Experiment description Decoy exosomes provide protection against bacterial toxins
Authors "Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K."
Journal name Nature
Publication year 2020
Sample Lung cancer cells
Sample name A549
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Flow cytometry
132
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
133
Experiment ID 108
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSP70|CD81|CD9|EpCAM
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21601258    
Organism Homo sapiens
Experiment description Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage.
Authors "Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P."
Journal name GO
Publication year 2011
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods "Differential centrifugation
Sucrose density gradient
Immunobeads (EPCAM, CD24)"
Flotation density 1.04-1.15 g/mL
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Immunoelctron microscopy
134
Experiment ID 55
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron Microscopy
PubMed ID 12147373    
Organism Homo sapiens
Experiment description Malignant effusions and immunogenic tumour-derived exosomes.
Authors "Andre F, Schartz NE, Movassagh M, Flament C, Pautier P, Morice P, Pomel C, Lhomme C, Escudier B, Le Chevalier T, Tursz T, Amigorena S, Raposo G, Angevin E, Zitvogel L"
Journal name LANCET
Publication year 2002
Sample Malignant pleural effusions
Sample name Malignant pleural effusions
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
135
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
136
Experiment ID 919
MISEV standards
✘ Biophysical techniques
✔
CD9|CD81|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
137
Experiment ID 41
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|MHCI|CD9|CD81|TFRC|LAMP2
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors "Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C"
Journal name INTIMM
Publication year 2005
Sample Mast cells
Sample name HMC-1
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
138
Experiment ID 71
MISEV standards
✘ Biophysical techniques
✔
RAB5B|LAMP2|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19801663    
Organism Homo sapiens
Experiment description Microenvironmental pH is a key factor for exosome traffic in tumor cells.
Authors "Parolini I, Federici C, Raggi C, Lugini L, Palleschi S, de Milito A, Coscia C, Iessi E, Logozzi MA, Molinari A, Colone M, Tatti M, Sargiacomo M, Fais S"
Journal name JBC
Publication year 2009
Sample Melanoma cells
Sample name Mel1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Thin layer chromatography
139
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
140
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
141
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
142
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
143
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
144
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
145
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
146
Experiment ID 922
MISEV standards
Biophysical techniques
✔
CD9|CD81|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 30918259    
Organism Homo sapiens
Experiment description Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
Authors "Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC."
Journal name Nat Commun
Publication year 2019
Sample Melanoma cells
Sample name SK-MEL-28
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
147
Experiment ID 1107
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD81|GAPDH
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-147
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Flow cytometry
148
Experiment ID 1108
MISEV standards
✘ Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-147 - Fraction 6
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
149
Experiment ID 1109
MISEV standards
✘ Biophysical techniques
✔
CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34957415    
Organism Homo sapiens
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name SK-MEL-147 - Fraction 7
Isolation/purification methods Differential centrifugation
OptiPrep density gradient centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
150
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
Western blotting
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
151
Experiment ID 261
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD81|LAMP2|CD9
Enriched markers
✘ Negative markers
✔
Y
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25669974    
Organism Homo sapiens
Experiment description "Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins,and metabolites"
Authors "Krishna C. Vallabhaneni1, Patrice Penfornis, Santosh Dhule, Francois Guillonneau, Kristen V. Adams, Yin Yuan Mo, Rui Xu, Yiming Liu, Kounosuke Watabe, Mohan C. Vemuri and Radhika Pochampally"
Journal name Oncotarget
Publication year 2014
Sample Mesenchymal stem cells
Sample name hMSCs
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Lipids
Metabolites
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
Sequencing
152
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
153
Experiment ID 493
MISEV standards
✘ Biophysical techniques
✔
CD81|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
154
Experiment ID 626
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9
Enriched markers
✔
GOLGA2
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 31489147    
Organism Homo sapiens
Experiment description Inflammation potentiates miR-939 expression and packaging into small extracellular vesicles
Authors "Ramanathan S, Shenoda BB, Lin Z, Alexander GM, Huppert A, Sacan A, Ajit SK."
Journal name J Extracell Vesicles
Publication year 2019
Sample Monocytes
Sample name THP-1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Immuno-EM
Western blotting
qRT-PCR
155
Experiment ID 626
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9
Enriched markers
✔
GOLGA2
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Immuno-EM
PubMed ID 31489147    
Organism Homo sapiens
Experiment description Inflammation potentiates miR-939 expression and packaging into small extracellular vesicles
Authors "Ramanathan S, Shenoda BB, Lin Z, Alexander GM, Huppert A, Sacan A, Ajit SK."
Journal name J Extracell Vesicles
Publication year 2019
Sample Monocytes
Sample name THP-1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Immuno-EM
Western blotting
qRT-PCR
156
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
157
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
158
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
159
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
160
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
161
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
162
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
163
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
164
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
165
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
166
Experiment ID 40
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
CD63|CD9|MHCII|TFRC|LAMP2|ITGA2B
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 15908444    
Organism Homo sapiens
Experiment description Exosomal-like vesicles are present in human blood plasma.
Authors "Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C"
Journal name INTIMM
Publication year 2005
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.15-1.27 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
Immunoelectron Microscopy
167
Experiment ID 123
MISEV standards
✔
IEM|EM
Biophysical techniques
✔
CD63|CD9|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21235781    
Organism Homo sapiens
Experiment description "Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages."
Authors "Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H."
Journal name JTM
Publication year 2011
Sample Plasma
Sample name Plasma - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
168
Experiment ID 536
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD9|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36455873    
Organism Homo sapiens
Experiment description Sevoflurane-induced POCD-associated exosomes delivered miR-584-5p regulates the growth of human microglia HMC3 cells through targeting BDNF
Authors "Zhao J, Zhang W, Wang S, Li Z, Huang Y, Li L."
Journal name Aging
Publication year 2022
Sample Plasma
Sample name Patients with post-operative cognitive dysfunction (POCD)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-qPCR
169
Experiment ID 537
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD9|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36455873    
Organism Homo sapiens
Experiment description Sevoflurane-induced POCD-associated exosomes delivered miR-584-5p regulates the growth of human microglia HMC3 cells through targeting BDNF
Authors "Zhao J, Zhang W, Wang S, Li Z, Huang Y, Li L."
Journal name Aging
Publication year 2022
Sample Plasma
Sample name Patients without post-operative cognitive dysfunction (non-POCD)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Small RNA sequencing
RT-qPCR
170
Experiment ID 1369
MISEV standards
Biophysical techniques
✔
CD63|CD81|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Plasma
Sample name Healthy donors
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
171
Experiment ID 1370
MISEV standards
Biophysical techniques
✔
CD81|CD63|CD9|TSG101|Alix
Enriched markers
✘ Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36631249    
Organism Homo sapiens
Experiment description S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos
Authors "Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO."
Journal name Gut
Publication year 2023
Sample Plasma
Sample name Hepatocellular carcinoma patients
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Immuno-EM
172
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
173
Experiment ID 868
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63
Enriched markers
✔
GAPDH
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35513391    
Organism Homo sapiens
Experiment description Small extracellular vesicle-mediated miR-320e transmission promotes osteogenesis in OPLL by targeting TAK1
Authors "Xu C, Zhang Z, Liu N, Li L, Zhong H, Wang R, Shi Q, Zhang Z, Wei L, Hu B, Zhang H, Shen X, Wang Y, Liu Y, Yuan W."
Journal name Nat Commun
Publication year 2022
Sample Posterior longitudinal ligament
Sample name Ossicifation of posterior longitudinal ligament cells
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RNA sequencing
qRT-PCR
174
Experiment ID 142
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
175
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
176
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
177
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
178
Experiment ID 250
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|TSG101|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors "Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D"
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
179
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometric analysis
Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
180
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometric analysis
Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
181
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
182
Experiment ID 122
MISEV standards
✔
IEM|EM
Biophysical techniques
✔
CD63|CD9|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21235781    
Organism Homo sapiens
Experiment description "Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages."
Authors "Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H."
Journal name JTM
Publication year 2011
Sample Saliva
Sample name Saliva - Normal
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
Immunoelectron Microscopy
Western blotting
183
Experiment ID 457
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|TSG101
Enriched markers
✔
CALR
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36517516    
Organism Homo sapiens
Experiment description Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes
Authors "Zhao D, Wu K, Sharma S, Xing F, Wu SY, Tyagi A, Deshpande R, Singh R, Wabitsch M, Mo YY, Watabe K."
Journal name Nat Commun
Publication year 2022
Sample Serum
Sample name Breast cancer patients - African American
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
184
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
185
Experiment ID 115
MISEV standards
✘ Biophysical techniques
✔
CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21505438    
Organism Homo sapiens
Experiment description Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors "Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F."
Journal name NATCOM
Publication year 2011
Sample T cells
Sample name J77
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Mass spectrometry
Microarray
186
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Flow cytometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
187
Experiment ID 118
MISEV standards
✘ Biophysical techniques
✔
SDCBP|GAPDH|CD9|CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21362503    
Organism Homo sapiens
Experiment description Protein profile of exosomes from trabecular meshwork cells.
Authors "Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL."
Journal name JOP
Publication year 2011
Sample Tabecular meshwork cells
Sample name Tabecular meshwork cells
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.07-1.2 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
188
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
189
Experiment ID 121
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|HSP60|HSP70|CD81
Enriched markers
✔
HSPA5
Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 21276792    
Organism Homo sapiens
Experiment description Morphologic and proteomic characterization of exosomes released by cultured extravillous trophoblast cells.
Authors "Atay S, Gercel-Taylor C, Kesimer M, Taylor DD."
Journal name ECR
Publication year 2011
Sample Trophoblast cells
Sample name Sw71
Isolation/purification methods Differential centrifugation
Ultrafiltration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
190
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
191
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
192
Experiment ID 77
MISEV standards
✘ Biophysical techniques
✔
CD81|CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
193
Experiment ID 78
MISEV standards
✘ Biophysical techniques
✔
CD81|CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Urine
Sample name Urine - Bladder cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
194
Experiment ID 888
MISEV standards
✘ Biophysical techniques
✔
CD63|TSG101|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33432243    
Organism Homo sapiens
Experiment description Exosome detection via the ultrafast-isolation system: EXODUS
Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F."
Journal name Nat Methods
Publication year 2021
Sample Urine
Sample name Urine
Isolation/purification methods Tangential flow filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
195
Experiment ID 894
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101|CD81
Enriched markers
✔
UMOD
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33432243    
Organism Homo sapiens
Experiment description Exosome detection via the ultrafast-isolation system: EXODUS
Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F."
Journal name Nat Methods
Publication year 2021
Sample Urine
Sample name Urine
Isolation/purification methods Tangential flow filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
196
Experiment ID 896
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101|CD81
Enriched markers
✔
UMOD
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33432243    
Organism Homo sapiens
Experiment description Exosome detection via the ultrafast-isolation system: EXODUS
Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F."
Journal name Nat Methods
Publication year 2021
Sample Urine
Sample name Urine
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
197
Experiment ID 897
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101|CD81
Enriched markers
✔
UMOD
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33432243    
Organism Homo sapiens
Experiment description Exosome detection via the ultrafast-isolation system: EXODUS
Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F."
Journal name Nat Methods
Publication year 2021
Sample Urine
Sample name Urine
Isolation/purification methods Filtration
Polyethylene glycol-based precipitation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
198
Experiment ID 899
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101|CD81
Enriched markers
✔
UMOD
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33432243    
Organism Homo sapiens
Experiment description Exosome detection via the ultrafast-isolation system: EXODUS
Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F."
Journal name Nat Methods
Publication year 2021
Sample Urine
Sample name Urine
Isolation/purification methods Filtration
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
199
Experiment ID 900
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|TSG101|CD81
Enriched markers
✔
UMOD
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33432243    
Organism Homo sapiens
Experiment description Exosome detection via the ultrafast-isolation system: EXODUS
Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F."
Journal name Nat Methods
Publication year 2021
Sample Urine
Sample name Urine
Isolation/purification methods Filtration
Phosphatidylserine affinity purification
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for CD81
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TFR2 7036
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 HRH1  
Two-hybrid Homo sapiens
3 F2RL1  
Two-hybrid Homo sapiens
4 LBR 3930
Two-hybrid Homo sapiens
5 TMX2 51075
Two-hybrid Homo sapiens
6 ITGB1 3688
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 RBBP6 5930
Two-hybrid Homo sapiens
8 DLG5 9231
Two-hybrid Homo sapiens
9 GPX8 493869
Two-hybrid Homo sapiens
10 VAMP3 9341
Affinity Capture-MS Homo sapiens
11 CSNK2B 1460
Two-hybrid Homo sapiens
12 CD19 930
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 GGT1 2678
Affinity Capture-Western Homo sapiens
14 FAM209A 200232
Two-hybrid Homo sapiens
15 ADRA1A  
Two-hybrid Homo sapiens
16 TP53 7157
Affinity Capture-MS Homo sapiens
17 MC4R  
Two-hybrid Homo sapiens
18 ZFPL1 7542
Affinity Capture-Western Homo sapiens
19 BST2 684
Two-hybrid Homo sapiens
20 GPR152  
Two-hybrid Homo sapiens
21 CD53 963
Affinity Capture-Western Homo sapiens
22 GJA4  
Two-hybrid Homo sapiens
23 CD4 920
Affinity Capture-Western Homo sapiens
24 PTGFRN 5738
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
25 ADRB2  
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
26 CD9 928
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
27 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
28 KIT 3815
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 GORAB 92344
Two-hybrid Homo sapiens
30 CHRM4  
Two-hybrid Homo sapiens
31 DACH1  
Affinity Capture-MS Homo sapiens
32 HNRNPD 3184
Two-hybrid Homo sapiens
33 RAB14 51552
Affinity Capture-MS Homo sapiens
34 CCR9  
Two-hybrid Homo sapiens
35 TSPAN4 7106
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 TMEM194A  
Two-hybrid Homo sapiens
37 GNB1 2782
Affinity Capture-MS Homo sapiens
38 CPLX4  
Two-hybrid Homo sapiens
39 IFITM1 8519
Affinity Capture-Western Homo sapiens
40 MYOC 4653
Two-hybrid Homo sapiens
41 GPR35  
Two-hybrid Homo sapiens
42 OXTR 5021
Two-hybrid Homo sapiens
43 JAK3 3718
Two-hybrid Homo sapiens
44 EBP  
Two-hybrid Homo sapiens
45 TSHR 7253
Two-hybrid Homo sapiens
46 Rnf128  
Affinity Capture-Western Mus musculus
47 ZBTB16  
Two-hybrid Homo sapiens
48 STX1A 6804
Two-hybrid Homo sapiens
49 CTSC 1075
Co-fractionation Homo sapiens
50 HTR4  
Two-hybrid Homo sapiens
51 INO80B 83444
Two-hybrid Homo sapiens
52 PDZK1IP1 10158
Two-hybrid Homo sapiens
53 IGSF8 93185
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
54 CCR2  
Two-hybrid Homo sapiens
55 MRPS18B 28973
Two-hybrid Homo sapiens
56 CD81 975
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
57 VKORC1 79001
Two-hybrid Homo sapiens
58 TNFRSF10C  
Affinity Capture-MS Homo sapiens
59 BDKRB2  
Two-hybrid Homo sapiens
60 SLC26A6 65010
Two-hybrid Homo sapiens
61 THAP4 51078
Two-hybrid Homo sapiens
62 TSPAN31 6302
Affinity Capture-MS Homo sapiens
63 CD63 967
Co-fractionation Homo sapiens
64 MCEE 84693
Two-hybrid Homo sapiens
65 LHFPL5  
Two-hybrid Homo sapiens
66 TREX1  
Two-hybrid Homo sapiens
67 CR2 1380
Affinity Capture-Western Homo sapiens
68 ERGIC3 51614
Two-hybrid Homo sapiens
69 CD82 3732
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
70 ITGA4 3676
Affinity Capture-Western Homo sapiens
71 SIRPA 140885
Reconstituted Complex Homo sapiens
72 CD46 4179
Affinity Capture-Western Homo sapiens
73 C6orf57  
Two-hybrid Homo sapiens
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