Gene description for PAFAH1B1
Gene name platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
Gene symbol PAFAH1B1
Other names/aliases LIS1
LIS2
MDCR
MDS
PAFAH
Species Homo sapiens
 Database cross references - PAFAH1B1
ExoCarta ExoCarta_5048
Entrez Gene 5048
HGNC 8574
MIM 601545
UniProt P43034  
 PAFAH1B1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for PAFAH1B1
Molecular Function
    protein homodimerization activity GO:0042803 ISS
    microtubule binding GO:0008017 ISS
    phosphoprotein binding GO:0051219 ISS
    protein binding GO:0005515 IPI
    phospholipase binding GO:0043274 ISS
    dynein intermediate chain binding GO:0045505 IEA
    dynactin binding GO:0034452 ISS
    dynein binding GO:0045502 IDA
    heparin binding GO:0008201 ISS
Biological Process
    vesicle transport along microtubule GO:0047496 ISS
    lipid catabolic process GO:0016042 IEA
    mitotic cell cycle GO:0000278 TAS
    actin cytoskeleton organization GO:0030036 ISS
    neuromuscular process controlling balance GO:0050885 IMP
    cerebral cortex development GO:0021987 IMP
    microtubule cytoskeleton organization GO:0000226 ISS
    negative regulation of neuron projection development GO:0010977 IEA
    osteoclast development GO:0036035 IEA
    layer formation in cerebral cortex GO:0021819 ISS
    acrosome assembly GO:0001675 ISS
    establishment of centrosome localization GO:0051660 IEA
    transmission of nerve impulse GO:0019226 ISS
    mitotic nuclear division GO:0007067 IEA
    neuroblast proliferation GO:0007405 ISS
    learning or memory GO:0007611 ISS
    hippocampus development GO:0021766 ISS
    positive regulation of cytokine-mediated signaling pathway GO:0001961 IEA
    ameboidal-type cell migration GO:0001667 IEA
    brain morphogenesis GO:0048854 IMP
    nuclear migration GO:0007097 IEA
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    cerebral cortex neuron differentiation GO:0021895 IEA
    adult locomotory behavior GO:0008344 IMP
    nuclear envelope disassembly GO:0051081 IEA
    positive regulation of mitotic cell cycle GO:0045931 IEA
    retrograde axon cargo transport GO:0008090 ISS
    neuron migration GO:0001764 ISS
    small GTPase mediated signal transduction GO:0007264 TAS
    positive regulation of axon extension GO:0045773 IEA
    corpus callosum morphogenesis GO:0021540 IMP
    microtubule organizing center organization GO:0031023 IMP
    protein secretion GO:0009306 IEA
    establishment of mitotic spindle orientation GO:0000132 IMP
    microtubule-based process GO:0007017 IDA
    organelle organization GO:0006996 TAS
    negative regulation of JNK cascade GO:0046329 IEA
    platelet activating factor metabolic process GO:0046469 ISS
    synaptic transmission GO:0007268 ISS
    stem cell division GO:0017145 IEA
Subcellular Localization
    kinesin complex GO:0005871 IEA
    axon GO:0030424 IEA
    centrosome GO:0005813 ISS
    nuclear envelope GO:0005635 IDA
    growth cone GO:0030426 IEA
    nuclear membrane GO:0031965 IEA
    cell cortex GO:0005938 IDA
    motile primary cilium GO:0031512 ISS
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 ISS
    microtubule associated complex GO:0005875 IDA
    neuronal cell body GO:0043025 IEA
    cell leading edge GO:0031252 ISS
    astral microtubule GO:0000235 IDA
    extracellular exosome GO:0070062 IDA
    kinetochore GO:0000776 IDA
 Experiment description of studies that identified PAFAH1B1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 282
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 283
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 286
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
8
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PAFAH1B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DCTN1 1639
Affinity Capture-Western Homo sapiens
2 CSNK2A1 1457
Biochemical Activity Homo sapiens
3 PAFAH1B1 5048
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 DYNC1H1 1778
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 NUDC 10726
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 TUBA1A 7846
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 DCX  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 PAFAH1B2 5049
Invitro Homo sapiens
Two-hybrid Homo sapiens
9 DCTN2 10540
Two-hybrid Homo sapiens
10 NDE1 54820
Invivo Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
11 NDEL1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
12 KARS 3735
Two-hybrid Homo sapiens
13 PAFAH1B3 5050
Two-hybrid Homo sapiens
Invitro Homo sapiens
14 RSN  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
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