ExoCarta: Gene summary

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Gene description for PRSS1
Gene name	protease, serine, 1 (trypsin 1)
Gene symbol	PRSS1
Other names/aliases	TRP1 TRY1 TRY4 TRYP1
Species	Homo sapiens
Database cross references - PRSS1
ExoCarta	ExoCarta_5644
Vesiclepedia	VP_5644
Entrez Gene	5644
HGNC	9475
MIM	276000
UniProt	P07477
PRSS1 identified in exosomes derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Chondrocytes	35931686
Chondrocytes	35931686

Gene ontology annotations for PRSS1

Molecular Function
	serine-type endopeptidase activity	GO:0004252	IBA
	serine-type endopeptidase activity	GO:0004252	TAS
	metal ion binding	GO:0046872	IEA
Biological Process
	proteolysis	GO:0006508	IBA
	digestion	GO:0007586	IEA
	extracellular matrix disassembly	GO:0022617	TAS
Subcellular Localization
	extracellular region	GO:0005576	NAS
	extracellular region	GO:0005576	TAS
	extracellular space	GO:0005615	IBA
	collagen-containing extracellular matrix	GO:0062023	HDA
	blood microparticle	GO:0072562	HDA

Experiment description of studies that identified PRSS1 in exosomes

Experiment ID

494

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD151\|CD63\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Osteoarthritic cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

496

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Healthy cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for PRSS1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

UBE2H

7328

Affinity Capture-MS

Homo sapiens

SNCA

6622

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRMT3

10196

Affinity Capture-MS

Homo sapiens

HSPA5

3309

Affinity Capture-MS

Homo sapiens

MYCN

Affinity Capture-MS

Homo sapiens

LGR4

55366

Affinity Capture-MS

Homo sapiens

KRT1

3848

Co-fractionation

Homo sapiens

Sod1

20655

Affinity Capture-MS

Mus musculus

CDC73

Affinity Capture-MS

Homo sapiens

PIWIL4

Affinity Capture-MS

Homo sapiens

LINC00839

Protein-RNA

Homo sapiens

PRDM5

Affinity Capture-MS

Homo sapiens

EPAS1

Affinity Capture-MS

Homo sapiens

BTF3

689

Affinity Capture-MS

Homo sapiens

KRT10

3858

Co-fractionation

Homo sapiens

ALB

213

Affinity Capture-MS

Homo sapiens

PRSS2

5645

Affinity Capture-MS

Homo sapiens

UBE3A

7337

Reconstituted Complex

Homo sapiens

KDM6A

Affinity Capture-MS

Homo sapiens

ZFYVE16

Proximity Label-MS

Homo sapiens

PARK2

Affinity Capture-MS

Homo sapiens

STK38L

23012

Affinity Capture-MS

Homo sapiens

CDY2A

Affinity Capture-MS

Homo sapiens

SERPINF2

5345

Biochemical Activity

Homo sapiens

PSMD14

10213

Affinity Capture-MS

Homo sapiens

RAD51

Affinity Capture-MS

Homo sapiens

FANCM

57697

Affinity Capture-MS

Homo sapiens

SEMG1

6406

Affinity Capture-MS

Homo sapiens

WTAP

9589

Affinity Capture-MS

Homo sapiens

CEP250

11190

Affinity Capture-MS

Homo sapiens

Sidt2

Affinity Capture-MS

Mus musculus

Dctn3

Affinity Capture-MS

Mus musculus

EFNA3

Affinity Capture-MS

Homo sapiens

DDX5

1655

Co-fractionation

Homo sapiens

PINK1

Affinity Capture-MS

Homo sapiens

SERPINB8

5271

Reconstituted Complex

Homo sapiens

ACPP

Affinity Capture-MS

Homo sapiens

Jup

16480

Affinity Capture-MS

Mus musculus

KRT2

3849

Co-fractionation

Homo sapiens

Nedd1

Affinity Capture-MS

Mus musculus

GATA2

Affinity Capture-MS

Homo sapiens

MYC

Affinity Capture-MS

Homo sapiens

Ufl1

Affinity Capture-MS

Mus musculus

VPS18

57617

Affinity Capture-MS

Homo sapiens

ADGRV1

84059

Affinity Capture-MS

Homo sapiens

HUWE1

10075

Affinity Capture-MS

Homo sapiens

NCAPG

64151

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which PRSS1 is involved

Pathway	Evidence	Source
Activation of Matrix Metalloproteinases	TAS	Reactome
Cobalamin (Cbl, vitamin B12) transport and metabolism	TAS	Reactome
Degradation of the extracellular matrix	TAS	Reactome
Extracellular matrix organization	TAS	Reactome
Metabolism	TAS	Reactome
Metabolism of vitamins and cofactors	TAS	Reactome
Metabolism of water-soluble vitamins and cofactors	TAS	Reactome
Uptake of dietary cobalamins into enterocytes	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here