Gene description for SHCBP1
Gene name SHC SH2-domain binding protein 1
Gene symbol SHCBP1
Other names/aliases PAL
Species Homo sapiens
 Database cross references - SHCBP1
ExoCarta ExoCarta_79801
Vesiclepedia VP_79801
Entrez Gene 79801
HGNC 29547
MIM 611027
UniProt Q8NEM2  
 SHCBP1 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SHCBP1
Molecular Function
    protein binding GO:0005515 IPI
    SH2 domain binding GO:0042169 IEA
Biological Process
    fibroblast growth factor receptor signaling pathway GO:0008543 IBA
    fibroblast growth factor receptor signaling pathway GO:0008543 ISS
    regulation of neural precursor cell proliferation GO:2000177 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    spindle GO:0005819 IEA
    midbody GO:0030496 IEA
 Experiment description of studies that identified SHCBP1 in exosomes
1
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SHCBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF574  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ZNF777  
Affinity Capture-MS Homo sapiens
4 SFN 2810
Affinity Capture-MS Homo sapiens
5 PSG9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MKI67  
Affinity Capture-MS Homo sapiens
7 SRSF11 9295
Co-fractionation Homo sapiens
8 ZNF136  
Affinity Capture-MS Homo sapiens
9 HSPA5 3309
Co-fractionation Homo sapiens
10 COPZ1 22818
Affinity Capture-MS Homo sapiens
11 ZNF331  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GYPB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NXF1 10482
Affinity Capture-RNA Homo sapiens
14 USP49 25862
Affinity Capture-MS Homo sapiens
15 Cd2ap 12488
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
16 Racgap1 26934
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
17 KIF14 9928
Affinity Capture-MS Homo sapiens
18 ANLN 54443
Affinity Capture-MS Homo sapiens
19 HSPA8 3312
Co-fractionation Homo sapiens
20 YWHAZ 7534
Affinity Capture-MS Homo sapiens
21 RPL36AL 6166
Affinity Capture-MS Homo sapiens
22 SMARCA5 8467
Co-fractionation Homo sapiens
23 RACGAP1 29127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ZNF276 92822
Affinity Capture-MS Homo sapiens
26 YWHAB 7529
Affinity Capture-MS Homo sapiens
27 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
28 PARK2  
Affinity Capture-MS Homo sapiens
29 KRBA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ZNF414  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CENPA  
Co-purification Homo sapiens
32 ZNF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 Dctn2 69654
Affinity Capture-MS Mus musculus
34 CAPZB 832
Affinity Capture-MS Homo sapiens
35 CHMP4B 128866
Affinity Capture-MS Homo sapiens
36 ZNF408  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KIF23 9493
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 PRKCA 5578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ZNF785  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ZUFSP 221302
Two-hybrid Homo sapiens
41 YWHAQ 10971
Affinity Capture-MS Homo sapiens
42 SULF2 55959
Affinity Capture-MS Homo sapiens
43 Shcbp1 20419
Affinity Capture-MS Mus musculus
44 Fgfr1op  
Affinity Capture-MS Mus musculus
45 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PRKCB 5579
Affinity Capture-MS Homo sapiens
47 RPA2 6118
Proximity Label-MS Homo sapiens
48 NOL9 79707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 ZNF563  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 Kif23 71819
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
51 ASIC5  
Affinity Capture-MS Homo sapiens
52 SHC1 6464
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which SHCBP1 is involved
No pathways found





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