Experiment description of studies that identified FAHD2A in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

5 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for FAHD2A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 MPST 4357 Co-fractionation Homo sapiens 2 DBT 1629 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 FAHD2B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 CAT 847 Co-fractionation Homo sapiens 5 ZNF287   Affinity Capture-MS Homo sapiens 6 ALDH7A1 501 Co-fractionation Homo sapiens 7 LCN8   Affinity Capture-MS Homo sapiens 8 SPRTN   Affinity Capture-MS Homo sapiens 9 SOD2 6648 Co-fractionation Homo sapiens Co-fractionation Homo sapiens 10 HMCES   Affinity Capture-MS Homo sapiens 11 TAS2R41   Affinity Capture-MS Homo sapiens 12 MSRB2   Co-fractionation Homo sapiens 13 OMP   Affinity Capture-MS Homo sapiens 14 UBE2H 7328 Affinity Capture-MS Homo sapiens 15 PKM 5315 Co-fractionation Homo sapiens 16 ISOC2   Co-fractionation Homo sapiens 17 LGALS3 3958 Co-fractionation Homo sapiens 18 UCHL1 7345 Co-fractionation Homo sapiens 19 ACAA2 10449 Co-fractionation Homo sapiens 20 PRDX5 25824 Co-fractionation Homo sapiens 21 THEM4   Co-fractionation Homo sapiens 22 BOLA3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 SETX   Affinity Capture-MS Homo sapiens 24 NENF 29937 Co-fractionation Homo sapiens 25 FCGR2A 2212 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 TST 7263 Co-fractionation Homo sapiens 27 PIR 8544 Co-fractionation Homo sapiens 28 CFL1 1072 Co-fractionation Homo sapiens 29 PARK7 11315 Co-fractionation Homo sapiens 30 SORD 6652 Co-fractionation Homo sapiens 31 HDHD3 81932 Co-fractionation Homo sapiens 32 SOX2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 FCER1A   Affinity Capture-MS Homo sapiens 34 GRHPR 9380 Co-fractionation Homo sapiens 35 MIF 4282 Co-fractionation Homo sapiens 36 CD7   Affinity Capture-MS Homo sapiens 37 ACOT1 641371 Co-fractionation Homo sapiens 38 HINT2 84681 Co-fractionation Homo sapiens 39 POLDIP2 26073 Co-fractionation Homo sapiens 40 DSTN 11034 Co-fractionation Homo sapiens 41 FH 2271 Co-fractionation Homo sapiens 42 ANXA7 310 Co-fractionation Homo sapiens 43 DDX58 23586 Affinity Capture-RNA Homo sapiens 44 WDFY1 57590 Co-fractionation Homo sapiens 45 CMPK1 51727 Co-fractionation Homo sapiens View the network

Pathways in which FAHD2A is involved

No pathways found