Gene description for AQP2
Gene name aquaporin 2 (collecting duct)
Gene symbol AQP2
Other names/aliases AQP-CD
WCH-CD
Species Homo sapiens
 Database cross references - AQP2
ExoCarta ExoCarta_359
Vesiclepedia VP_359
Entrez Gene 359
HGNC 634
MIM 107777
UniProt P41181  
 AQP2 identified in sEVs derived from the following tissue/cell type
Amniotic fluid 17700640    
Urine 15326289    
Urine 17700640    
Urine 19056867    
Urine 21082674    
Urine 21557262    
Urine 21557262    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for AQP2
Molecular Function
    water transmembrane transporter activity GO:0005372 IDA
    protein binding GO:0005515 IPI
    glycerol transmembrane transporter activity GO:0015168 IDA
    water channel activity GO:0015250 EXP
    water channel activity GO:0015250 IBA
    water channel activity GO:0015250 IMP
Biological Process
    renal water homeostasis GO:0003091 IMP
    renal water homeostasis GO:0003091 TAS
    renal water transport GO:0003097 IEA
    water transport GO:0006833 IBA
    water transport GO:0006833 IDA
    water transport GO:0006833 IMP
    actin filament organization GO:0007015 IMP
    glycerol transmembrane transport GO:0015793 IDA
    cellular response to water deprivation GO:0042631 IEA
    protein homotetramerization GO:0051289 IDA
    cellular response to copper ion GO:0071280 IDA
    cellular response to mercury ion GO:0071288 IDA
    metanephric collecting duct development GO:0072205 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IEA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    transport vesicle membrane GO:0030658 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    recycling endosome GO:0055037 IEA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
    lumenal side of membrane GO:0098576 IEA
 Experiment description of studies that identified AQP2 in sEVs
1
Experiment ID 58
MISEV standards
✘ Biophysical techniques
✔
HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Homo sapiens
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors "Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name KI
Publication year 2007
Sample Amniotic fluid
Sample name Amniotic fluid
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 57
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Homo sapiens
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors "Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name KI
Publication year 2007
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.05-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
5
Experiment ID 102
MISEV standards
✔
IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21082674    
Organism Homo sapiens
Experiment description "Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS."
Authors "Zhang Y, Li Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
6
Experiment ID 113
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 114
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors "Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AQP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDIPT 10423
Two-hybrid Homo sapiens
2 ELL2 22936
Two-hybrid Homo sapiens
3 TMEM60  
Two-hybrid Homo sapiens
4 OR10AG1  
Two-hybrid Homo sapiens
5 MRM1  
Two-hybrid Homo sapiens
6 GPX8 493869
Two-hybrid Homo sapiens
7 SSR3 6747
Two-hybrid Homo sapiens
8 SLC30A2  
Two-hybrid Homo sapiens
9 CCDC167  
Two-hybrid Homo sapiens
10 VAPB 9217
Two-hybrid Homo sapiens
11 TMEM218  
Two-hybrid Homo sapiens
12 NEDD4 4734
Affinity Capture-Western Homo sapiens
13 C14orf1 11161
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 YIPF6 286451
Two-hybrid Homo sapiens
15 PANX1 24145
Two-hybrid Homo sapiens
16 NDFIP2 54602
Two-hybrid Homo sapiens
17 INSIG2  
Two-hybrid Homo sapiens
18 TM9SF2 9375
Two-hybrid Homo sapiens
19 RTP2  
Two-hybrid Homo sapiens
20 LEUTX  
Two-hybrid Homo sapiens
21 SLC26A6 65010
Two-hybrid Homo sapiens
22 SUPT20H 55578
Two-hybrid Homo sapiens
23 ERGIC3 51614
Two-hybrid Homo sapiens
24 BCAP31 10134
Two-hybrid Homo sapiens
25 TMEM236  
Two-hybrid Homo sapiens
26 MUC1 4582
Two-hybrid Homo sapiens
27 SLC5A2 6524
Two-hybrid Homo sapiens
28 CLDN7 1366
Two-hybrid Homo sapiens
29 IGFBP5 3488
Two-hybrid Homo sapiens
30 Ndfip2  
Affinity Capture-Western Mus musculus
31 IER3  
Two-hybrid Homo sapiens
32 EFNA5  
Two-hybrid Homo sapiens
33 OLFM4 10562
Two-hybrid Homo sapiens
34 SLC38A7 55238
Two-hybrid Homo sapiens
35 CREB3L1  
Two-hybrid Homo sapiens
36 PNLIPRP1  
Two-hybrid Homo sapiens
37 SNAP47 116841
Two-hybrid Homo sapiens
38 FAM209A 200232
Two-hybrid Homo sapiens
39 TUSC5  
Two-hybrid Homo sapiens
40 SPCS1 28972
Two-hybrid Homo sapiens
41 COL4A3BP 10087
Two-hybrid Homo sapiens
42 STX8 9482
Two-hybrid Homo sapiens
43 TMEM109 79073
Two-hybrid Homo sapiens
44 Nedd4l 83814
Affinity Capture-Western Mus musculus
45 C4orf3  
Two-hybrid Homo sapiens
46 TMEM230 29058
Two-hybrid Homo sapiens
47 CREB3  
Two-hybrid Homo sapiens
48 PDZK1IP1 10158
Two-hybrid Homo sapiens
49 TMEM86A  
Two-hybrid Homo sapiens
50 ACTB 60
Two-hybrid Homo sapiens
51 PLP2 5355
Two-hybrid Homo sapiens
52 GPAA1 8733
Two-hybrid Homo sapiens
53 Ndfip1  
Affinity Capture-Western Mus musculus
54 LHFPL5  
Two-hybrid Homo sapiens
55 MS4A13  
Two-hybrid Homo sapiens
56 SNX5 27131
Two-hybrid Homo sapiens
57 TMEM97 27346
Two-hybrid Homo sapiens
58 CXCL16 58191
Two-hybrid Homo sapiens
59 UBE2J1 51465
Two-hybrid Homo sapiens
60 EEF1A1 1915
Two-hybrid Homo sapiens
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