Gene description for AQP2
Gene name
aquaporin 2 (collecting duct)
Gene symbol
AQP2
Other names/aliases
AQP-CD WCH-CD
Species
Homo sapiens
Database cross references - AQP2
ExoCarta
ExoCarta_359
Vesiclepedia
VP_359
Entrez Gene
359
HGNC
634
MIM
107777
UniProt
P41181
AQP2 identified in exosomes derived from the following tissue/cell type
Amniotic fluid
17700640
Urine
15326289
Urine
17700640
Urine
19056867
Urine
21082674
Urine
21557262
Urine
21557262
Urine
21595033
Urine
21595033
Urine
21595033
Urine
22418980
Urine
22418980
Gene ontology annotations for AQP2
Experiment description of studies that identified AQP2 in exosomes
1
Experiment ID
58
MISEV standards
✘
Biophysical techniques
✔
HSP70|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
17700640
Organism
Homo sapiens
Experiment description
CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors
"Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name
KI
Publication year
2007
Sample
Amniotic fluid
Sample name
Amniotic fluid
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.08-1.14 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting
2
Experiment ID
13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15326289
Organism
Homo sapiens
Experiment description
Identification and proteomic profiling of exosomes in human urine.
Authors
"Pisitkun T, Shen RF, Knepper MA"
Journal name
PNAS
Publication year
2004
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA XP] Western blotting
3
Experiment ID
57
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
17700640
Organism
Homo sapiens
Experiment description
CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors
"Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P"
Journal name
KI
Publication year
2007
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.05-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS
4
Experiment ID
63
MISEV standards
✘
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
5
Experiment ID
102
MISEV standards
✔
IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Immunoelectron microscopy
PubMed ID
21082674
Organism
Homo sapiens
Experiment description
"Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS."
Authors
"Zhang Y, Li Y, Qiu F, Qiu Z."
Journal name
ELEC
Publication year
2010
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Immunoelectron Microscopy
6
Experiment ID
113
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Immunoelectron microscopy
PubMed ID
21557262
Organism
Homo sapiens
Experiment description
Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors
"Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name
ELEC
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
7
Experiment ID
114
MISEV standards
✔
IEM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Immunoelectron microscopy
PubMed ID
21557262
Organism
Homo sapiens
Experiment description
Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors
"Li Y, Zhang Y, Qiu F, Qiu Z."
Journal name
ELEC
Publication year
2011
Sample
Urine
Sample name
Urine - Lung cancer
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
8
Experiment ID
193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
9
Experiment ID
194
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of basement membrane nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
10
Experiment ID
195
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21595033
Organism
Homo sapiens
Experiment description
Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors
"Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name
Proteomics
Publication year
2011
Sample
Urine
Sample name
Urine - Patients of early IgA nephropathy
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
"Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal high density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
12
Experiment ID
197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
"Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal low density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for AQP2
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
CDIPT
10423
Two-hybrid
Homo sapiens
2
ELL2
22936
Two-hybrid
Homo sapiens
3
TMEM60
Two-hybrid
Homo sapiens
4
OR10AG1
Two-hybrid
Homo sapiens
5
MRM1
Two-hybrid
Homo sapiens
6
GPX8
493869
Two-hybrid
Homo sapiens
7
SSR3
6747
Two-hybrid
Homo sapiens
8
SLC30A2
Two-hybrid
Homo sapiens
9
CCDC167
Two-hybrid
Homo sapiens
10
VAPB
9217
Two-hybrid
Homo sapiens
11
TMEM218
Two-hybrid
Homo sapiens
12
NEDD4
4734
Affinity Capture-Western
Homo sapiens
13
C14orf1
11161
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
14
YIPF6
286451
Two-hybrid
Homo sapiens
15
PANX1
24145
Two-hybrid
Homo sapiens
16
NDFIP2
54602
Two-hybrid
Homo sapiens
17
INSIG2
Two-hybrid
Homo sapiens
18
TM9SF2
9375
Two-hybrid
Homo sapiens
19
RTP2
Two-hybrid
Homo sapiens
20
LEUTX
Two-hybrid
Homo sapiens
21
SLC26A6
65010
Two-hybrid
Homo sapiens
22
SUPT20H
55578
Two-hybrid
Homo sapiens
23
ERGIC3
51614
Two-hybrid
Homo sapiens
24
BCAP31
10134
Two-hybrid
Homo sapiens
25
TMEM236
Two-hybrid
Homo sapiens
26
MUC1
4582
Two-hybrid
Homo sapiens
27
SLC5A2
6524
Two-hybrid
Homo sapiens
28
CLDN7
1366
Two-hybrid
Homo sapiens
29
IGFBP5
3488
Two-hybrid
Homo sapiens
30
Ndfip2
Affinity Capture-Western
Mus musculus
31
IER3
Two-hybrid
Homo sapiens
32
EFNA5
Two-hybrid
Homo sapiens
33
OLFM4
10562
Two-hybrid
Homo sapiens
34
SLC38A7
55238
Two-hybrid
Homo sapiens
35
CREB3L1
Two-hybrid
Homo sapiens
36
PNLIPRP1
Two-hybrid
Homo sapiens
37
SNAP47
116841
Two-hybrid
Homo sapiens
38
FAM209A
200232
Two-hybrid
Homo sapiens
39
TUSC5
Two-hybrid
Homo sapiens
40
SPCS1
28972
Two-hybrid
Homo sapiens
41
COL4A3BP
10087
Two-hybrid
Homo sapiens
42
STX8
9482
Two-hybrid
Homo sapiens
43
TMEM109
79073
Two-hybrid
Homo sapiens
44
Nedd4l
83814
Affinity Capture-Western
Mus musculus
45
C4orf3
Two-hybrid
Homo sapiens
46
TMEM230
29058
Two-hybrid
Homo sapiens
47
CREB3
Two-hybrid
Homo sapiens
48
PDZK1IP1
10158
Two-hybrid
Homo sapiens
49
TMEM86A
Two-hybrid
Homo sapiens
50
ACTB
60
Two-hybrid
Homo sapiens
51
PLP2
5355
Two-hybrid
Homo sapiens
52
GPAA1
8733
Two-hybrid
Homo sapiens
53
Ndfip1
Affinity Capture-Western
Mus musculus
54
LHFPL5
Two-hybrid
Homo sapiens
55
MS4A13
Two-hybrid
Homo sapiens
56
SNX5
27131
Two-hybrid
Homo sapiens
57
TMEM97
27346
Two-hybrid
Homo sapiens
58
CXCL16
58191
Two-hybrid
Homo sapiens
59
UBE2J1
51465
Two-hybrid
Homo sapiens
60
EEF1A1
1915
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which AQP2 is involved