Gene description for AQP2
Gene name aquaporin 2 (collecting duct)
Gene symbol AQP2
Other names/aliases AQP-CD
WCH-CD
Species Homo sapiens
 Database cross references - AQP2
ExoCarta ExoCarta_359
Entrez Gene 359
HGNC 634
MIM 107777
UniProt P41181  
 AQP2 identified in exosomes derived from the following tissue/cell type
Amniotic fluid 17700640    
Urine 15326289    
Urine 17700640    
Urine 19056867    
Urine 21082674    
Urine 21557262    
Urine 21557262    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for AQP2
Molecular Function
    PDZ domain binding GO:0030165 IEA
    actin binding GO:0003779 IEA
    water channel activity GO:0015250 EXP
    glycerol channel activity GO:0015254 IBA
    water transmembrane transporter activity GO:0005372 IDA
    glycerol transmembrane transporter activity GO:0015168 IDA
Biological Process
    cellular response to mercury ion GO:0071288 IDA
    cellular response to water deprivation GO:0042631 IEA
    response to salt stress GO:0009651 IEA
    female pregnancy GO:0007565 IEA
    response to lipopolysaccharide GO:0032496 IEA
    glycerol transport GO:0015793 IDA
    renal water transport GO:0003097 IEA
    ion transmembrane transport GO:0034220 IBA
    cellular water homeostasis GO:0009992 IBA
    aging GO:0007568 IEA
    excretion GO:0007588 TAS
    cellular response to copper ion GO:0071280 IDA
    water transport GO:0006833 TAS
    transmembrane transport GO:0055085 TAS
    response to lithium ion GO:0010226 IEA
    response to glucagon GO:0033762 IEA
    response to starvation GO:0042594 IEA
    apoptotic process GO:0006915 IEA
    metanephric collecting duct development GO:0072205 IEA
    actin filament depolymerization GO:0030042 IEA
    renal water homeostasis GO:0003091 TAS
    positive regulation of calcium ion transport GO:0051928 IEA
    hyperosmotic response GO:0006972 IEA
    response to calcium ion GO:0051592 IEA
Subcellular Localization
    protein complex GO:0043234 IEA
    membrane GO:0016020 IDA
    transport vesicle membrane GO:0030658 TAS
    integral component of plasma membrane GO:0005887 IBA
    clathrin-coated vesicle GO:0030136 IEA
    extracellular exosome GO:0070062 IDA
    early endosome GO:0005769 IEA
    plasma membrane GO:0005886 TAS
    basolateral plasma membrane GO:0016323 IEA
    exocytic vesicle GO:0070382 IEA
    trans-Golgi network GO:0005802 IEA
    rough endoplasmic reticulum GO:0005791 IEA
    lysosome GO:0005764 IEA
    recycling endosome GO:0055037 IEA
    apical plasma membrane GO:0016324 ISS
 Experiment description of studies that identified AQP2 in exosomes
1
Experiment ID 58
ISEV standards
✘ EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Homo sapiens
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P
Journal name KI
Publication year 2007
Sample Amniotic fluid
Sample name Amniotic fluid
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 57
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 17700640    
Organism Homo sapiens
Experiment description CD24 is a marker of exosomes secreted into urine and amniotic fluid.
Authors Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P
Journal name KI
Publication year 2007
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.05-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
FACS
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
5
Experiment ID 102
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21082674    
Organism Homo sapiens
Experiment description Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS.
Authors Zhang Y, Li Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
6
Experiment ID 113
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors Li Y, Zhang Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 114
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Immunoelectron microscopy
PubMed ID 21557262    
Organism Homo sapiens
Experiment description Proteomic identification of exosomal LRG1: A potential urinary biomarker for detecting NSCLC.
Authors Li Y, Zhang Y, Qiu F, Qiu Z.
Journal name ELEC
Publication year 2011
Sample Urine
Sample name Urine - Lung cancer
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AQP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which AQP2 is involved
PathwayEvidenceSource
Passive transport by Aquaporins TAS Reactome
Vasopressin regulates renal water homeostasis via Aquaporins TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here