Gene description for AKAP11
Gene name A kinase (PRKA) anchor protein 11
Gene symbol AKAP11
Other names/aliases AKAP-11
AKAP220
PPP1R44
PRKA11
Species Homo sapiens
 Database cross references - AKAP11
ExoCarta ExoCarta_11215
Entrez Gene 11215
HGNC 369
MIM 604696
UniProt Q9UKA4  
 AKAP11 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for AKAP11
Molecular Function
    protein kinase A binding GO:0051018 TAS
    protein phosphatase 1 binding GO:0008157 TAS
Biological Process
    intracellular signal transduction GO:0035556 TAS
    regulation of protein kinase A signaling GO:0010738 IBA
Subcellular Localization
    microtubule organizing center GO:0005815 IEA
    cytoplasm GO:0005737 IBA
    peroxisome GO:0005777 IBA
 Experiment description of studies that identified AKAP11 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for AKAP11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMYD2  
Affinity Capture-MS Homo sapiens
2 PPP1CC 5501
Affinity Capture-Western Homo sapiens
3 PPP1CA 5499
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 PRKAR2A 5576
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 PRKAR1A 5573
Invivo Homo sapiens
6 PPP1CB 5500
Affinity Capture-Western Homo sapiens
7 GSK3B 2932
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 PRKAR2B 5577
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which AKAP11 is involved
No pathways found





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