Experiment description of studies that identified AKAP11 in exosomes

1 Experiment ID 226 ISEV standards ✔ EM EV Biophysical techniques ✔ GAPDH EV Cytosolic markers ✔ CD9|FLOT1 EV Membrane markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 EV Negative markers ✘ EV Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 236 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for AKAP11

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SMYD2   Affinity Capture-MS Homo sapiens 2 PPP1CC 5501 Affinity Capture-Western Homo sapiens 3 PPP1CA 5499 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 4 PRKAR2A 5576 Protein-peptide Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 5 PRKAR1A 5573 Invivo Homo sapiens 6 PPP1CB 5500 Affinity Capture-Western Homo sapiens 7 GSK3B 2932 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 8 PRKAR2B 5577 Invivo Homo sapiens View the network

Pathways in which AKAP11 is involved

No pathways found