Gene description for SLA2
Gene name Src-like-adaptor 2
Gene symbol SLA2
Other names/aliases C20orf156
MARS
SLAP-2
SLAP2
Species Homo sapiens
 Database cross references - SLA2
ExoCarta ExoCarta_84174
Vesiclepedia VP_84174
Entrez Gene 84174
HGNC 17329
MIM 606577
UniProt Q9H6Q3  
 SLA2 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SLA2
Molecular Function
    protein binding GO:0005515 IPI
    signaling adaptor activity GO:0035591 IBA
    signaling adaptor activity GO:0035591 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    B cell mediated immunity GO:0019724 TAS
    T cell activation GO:0042110 IDA
    regulation of immune response GO:0050776 NAS
    negative regulation of calcium-mediated signaling GO:0050849 IDA
    antigen receptor-mediated signaling pathway GO:0050851 TAS
    negative regulation of T cell receptor signaling pathway GO:0050860 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 TAS
    late endosome GO:0005770 IEA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    endosome membrane GO:0010008 NAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified SLA2 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MET 4233
Reconstituted Complex Homo sapiens
2 GAB1  
Reconstituted Complex Homo sapiens
3 CD247 919
Affinity Capture-Western Homo sapiens
4 CBL 867
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
5 RPSAP58 388524
Affinity Capture-MS Homo sapiens
6 PRKAA2 5563
Two-hybrid Homo sapiens
7 ALB 213
Affinity Capture-MS Homo sapiens
8 KIT 3815
Reconstituted Complex Homo sapiens
9 C5orf30  
Affinity Capture-MS Homo sapiens
10 ERBB4 2066
Reconstituted Complex Homo sapiens
11 ERBB2 2064
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
12 UBASH3A 53347
Two-hybrid Homo sapiens
13 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Reconstituted Complex Homo sapiens
14 CSF1 1435
Reconstituted Complex Homo sapiens
15 TARBP1 6894
Affinity Capture-MS Homo sapiens
16 DCAF10  
Affinity Capture-MS Homo sapiens
17 ERBB3 2065
Reconstituted Complex Homo sapiens
18 BCR 613
Affinity Capture-MS Homo sapiens
19 LAMC2 3918
Far Western Homo sapiens
20 ZAP70 7535
Affinity Capture-Western Homo sapiens
21 UNC119B 84747
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SLA2 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
FLT3 Signaling TAS Reactome
Immune System TAS Reactome
Negative regulation of FLT3 TAS Reactome





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