Gene description for WDR4
Gene name WD repeat domain 4
Gene symbol WDR4
Other names/aliases TRM82
TRMT82
Species Homo sapiens
 Database cross references - WDR4
ExoCarta ExoCarta_10785
Vesiclepedia VP_10785
Entrez Gene 10785
HGNC 12756
MIM 605924
UniProt P57081  
 WDR4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for WDR4
Molecular Function
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 IDA
    tRNA methyltransferase activator activity GO:0141106 IDA
Biological Process
    tRNA modification GO:0006400 IBA
    tRNA modification GO:0006400 IDA
    DNA damage response GO:0006974 IEA
    tRNA (guanine-N7)-methylation GO:0106004 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 HDA
    nucleoplasm GO:0005654 IDA
    chromosome GO:0005694 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    tRNA methyltransferase complex GO:0043527 IBA
    tRNA methyltransferase complex GO:0043527 IDA
    tRNA (m7G46) methyltransferase complex GO:0106143 IDA
 Experiment description of studies that identified WDR4 in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDR4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Co-fractionation Homo sapiens
2 ACTR2 10097
Co-fractionation Homo sapiens
3 PDE12 201626
Co-fractionation Homo sapiens
4 BIRC6 57448
Affinity Capture-MS Homo sapiens
5 PBX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 NPLOC4 55666
Co-fractionation Homo sapiens
7 SIRT6  
Affinity Capture-MS Homo sapiens
8 LGR4 55366
Affinity Capture-MS Homo sapiens
9 FAM118A  
Two-hybrid Homo sapiens
10 FEN1 2237
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 ADSL 158
Co-fractionation Homo sapiens
12 SAR1A 56681
Co-fractionation Homo sapiens
13 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
14 PAFAH1B2 5049
Co-fractionation Homo sapiens
15 HSPBP1 23640
Co-fractionation Homo sapiens
16 RPA2 6118
Proximity Label-MS Homo sapiens
17 UBQLN2 29978
Co-fractionation Homo sapiens
18 WDR92 116143
Co-fractionation Homo sapiens
19 ALG12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 DPP8 54878
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
21 CUL5 8065
Co-fractionation Homo sapiens
22 DBNL 28988
Co-fractionation Homo sapiens
23 ERO1L 30001
Co-fractionation Homo sapiens
24 HIST1H2BB 3018
Affinity Capture-MS Homo sapiens
25 RNF138  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TSFM 10102
Co-fractionation Homo sapiens
27 ELAC2 60528
Co-fractionation Homo sapiens
28 PARK2  
Affinity Capture-MS Homo sapiens
29 SAE1 10055
Co-fractionation Homo sapiens
30 RABGGTB 5876
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 ALAD 210
Co-fractionation Homo sapiens
32 UBE2R2 54926
Co-fractionation Homo sapiens
33 EIF2A 83939
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
34 PCNA 5111
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
35 CENPO  
Affinity Capture-MS Homo sapiens
36 SURF2  
Co-fractionation Homo sapiens
37 ASMTL 8623
Co-fractionation Homo sapiens
38 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
39 XPO5 57510
Co-fractionation Homo sapiens
40 TERF1 7013
Two-hybrid Homo sapiens
41 XPNPEP1 7511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 UBP1 7342
Co-fractionation Homo sapiens
43 KIF1BP 26128
Co-fractionation Homo sapiens
44 RPA3 6119
Proximity Label-MS Homo sapiens
45 DDX5 1655
Co-fractionation Homo sapiens
46 OGT 8473
Co-fractionation Homo sapiens
47 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 TERF2  
Two-hybrid Homo sapiens
49 HIST1H2AB 8335
Affinity Capture-MS Homo sapiens
50 PLIN3 10226
Co-fractionation Homo sapiens
51 CARM1 10498
Affinity Capture-MS Homo sapiens
52 TP53RK 112858
Co-fractionation Homo sapiens
53 PPME1 51400
Co-fractionation Homo sapiens
54 ERC1 23085
Co-fractionation Homo sapiens
55 TCEAL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 TRIM28 10155
Affinity Capture-RNA Homo sapiens
57 OBSL1 23363
Affinity Capture-MS Homo sapiens
58 PPP2R2A 5520
Co-fractionation Homo sapiens
59 DNAJB1 3337
Co-fractionation Homo sapiens
60 SRP14 6727
Co-fractionation Homo sapiens
61 METTL1 4234
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
62 DCTPP1 79077
Co-fractionation Homo sapiens
63 ARIH1 25820
Co-fractionation Homo sapiens
64 RPS16 6217
Affinity Capture-MS Homo sapiens
65 UBE2D1 7321
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which WDR4 is involved
PathwayEvidenceSource
Metabolism of RNA IEA Reactome
tRNA modification in the nucleus and cytosol IEA Reactome
tRNA processing IEA Reactome





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