Gene description for LRP1
Gene name low density lipoprotein receptor-related protein 1
Gene symbol LRP1
Other names/aliases A2MR
APOER
APR
CD91
IGFBP3R
LRP
LRP1A
TGFBR5
Species Homo sapiens
 Database cross references - LRP1
ExoCarta ExoCarta_4035
Entrez Gene 4035
HGNC 6692
MIM 107770
UniProt Q07954  
 LRP1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for LRP1
Molecular Function
    apolipoprotein binding GO:0034185 IPI
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 IDA
    poly(A) RNA binding GO:0044822 IDA
    lipoprotein particle receptor binding GO:0070325 IC
    receptor activity GO:0004872 TAS
    protease binding GO:0002020 IEA
    calcium ion binding GO:0005509 ISS
    lipoprotein transporter activity GO:0042954 NAS
Biological Process
    positive regulation of protein transport GO:0051222 IEA
    protein kinase C-activating G-protein coupled receptor signaling pathway GO:0007205 IEA
    regulation of actin cytoskeleton organization GO:0032956 ISS
    beta-amyloid clearance GO:0097242 TAS
    receptor-mediated endocytosis GO:0006898 TAS
    negative regulation of neuron apoptotic process GO:0043524 IEA
    negative regulation of smooth muscle cell migration GO:0014912 ISS
    regulation of cholesterol transport GO:0032374 ISS
    negative regulation of Wnt signaling pathway GO:0030178 ISS
    positive regulation of cholesterol efflux GO:0010875 ISS
    cell proliferation GO:0008283 IEA
    phototransduction, visible light GO:0007603 TAS
    cholesterol metabolic process GO:0008203 IEA
    aging GO:0007568 IEA
    regulation of phospholipase A2 activity GO:0032429 ISS
    lipoprotein metabolic process GO:0042157 IEA
    apoptotic cell clearance GO:0043277 ISS
    positive regulation of lipid transport GO:0032370 ISS
    lipoprotein transport GO:0042953 NAS
    retinoid metabolic process GO:0001523 TAS
    negative regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000587 ISS
    aorta morphogenesis GO:0035909 ISS
Subcellular Localization
    nucleolus GO:0005730 IDA
    coated pit GO:0005905 IEA
    focal adhesion GO:0005925 IDA
    clathrin-coated vesicle GO:0030136 IEA
    integral component of plasma membrane GO:0005887 ISS
    endocytic vesicle membrane GO:0030666 TAS
    plasma membrane GO:0005886 TAS
    receptor complex GO:0043235 IDA
    endosome GO:0005768 IEA
    neuronal cell body GO:0043025 IEA
    lysosomal membrane GO:0005765 IDA
    cytoplasm GO:0005737 IDA
    dendrite GO:0030425 IEA
 Experiment description of studies that identified LRP1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 44
ISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
10
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LRP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 A2M 2
Invivo Homo sapiens
Co-crystal Structure Homo sapiens
2 CANX 821
Affinity Capture-Western Homo sapiens
3 CALR 811
Affinity Capture-Western Homo sapiens
4 PLAT 5327
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
5 DAB2 1601
Reconstituted Complex Homo sapiens
6 PDGFB  
Reconstituted Complex Homo sapiens
7 FURIN 5045
Invivo Homo sapiens
Invitro Homo sapiens
8 GIPC1 10755
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
9 MAPK8IP2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
10 GULP1 51454
Reconstituted Complex Homo sapiens
11 MDK 4192
Affinity Capture-MS Homo sapiens
12 ANAPC10  
Two-hybrid Homo sapiens
13 CALCR  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 PLAUR 5329
Invitro Homo sapiens
15 ITGB1BP1 9270
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
16 DAB1 1600
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
17 C4BPA 722
Invivo Homo sapiens
Invitro Homo sapiens
18 SERPINE1 5054
Affinity Capture-Western Homo sapiens
19 DLG4  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
20 APBB1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
21 APP 351
Invivo Homo sapiens
Invivo Homo sapiens
22 SCN3A  
Two-hybrid Homo sapiens
23 MAPK8IP1 9479
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
24 HTATIP  
FRET Homo sapiens
25 APBA2 321
Two-hybrid Homo sapiens
26 SNX17 9784
Invivo Homo sapiens
Invitro Homo sapiens
27 LIPC  
Invivo Homo sapiens
Invitro Homo sapiens
28 SYNJ2BP  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
29 C3 718
Invivo Homo sapiens
30 THBS1 7057
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
31 SRC 6714
Biochemical Activity Homo sapiens
32 F9 2158
Invitro Homo sapiens
33 CAPON  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
34 LTF 4057
Invivo Homo sapiens
35 LPL 4023
Invitro Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
36 LRPAP1  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
37 MMP14 4323
Affinity Capture-Western Homo sapiens
38 F8 2157
Reconstituted Complex Homo sapiens
39 PDIA2  
Affinity Capture-Western Homo sapiens
40 CD82 3732
Invivo Homo sapiens
Invitro Homo sapiens
41 SHC1 6464
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which LRP1 is involved
PathwayEvidenceSource
Retinoid metabolism and transport TAS Reactome
Scavenging of heme from plasma TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here