Gene description for MTR
Gene name 5-methyltetrahydrofolate-homocysteine methyltransferase
Gene symbol MTR
Other names/aliases HMAG
MS
cblG
Species Homo sapiens
 Database cross references - MTR
ExoCarta ExoCarta_4548
Vesiclepedia VP_4548
Entrez Gene 4548
HGNC 7468
MIM 156570
UniProt Q99707  
 MTR identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MTR
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    methionine synthase activity GO:0008705 IBA
    methionine synthase activity GO:0008705 IDA
    methionine synthase activity GO:0008705 IMP
    methionine synthase activity GO:0008705 TAS
    cobalamin binding GO:0031419 IEA
Biological Process
    sulfur amino acid metabolic process GO:0000096 TAS
    nervous system development GO:0007399 TAS
    methionine biosynthetic process GO:0009086 IBA
    methionine biosynthetic process GO:0009086 IMP
    cobalamin metabolic process GO:0009235 IMP
    cobalamin metabolic process GO:0009235 TAS
    axon regeneration GO:0031103 ISS
    methylation GO:0032259 TAS
    tetrahydrofolate metabolic process GO:0046653 IBA
    response to axon injury GO:0048678 ISS
    homocysteine metabolic process GO:0050667 IBA
    cellular response to nitric oxide GO:0071732 ISS
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MTR in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MTR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 FAM196A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DHFR 1719
Affinity Capture-MS Homo sapiens
5 DPP9 91039
Co-fractionation Homo sapiens
6 NPAS1  
Affinity Capture-MS Homo sapiens
7 LINC01587  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 LGI1  
Affinity Capture-MS Homo sapiens
9 LAMTOR1 55004
Proximity Label-MS Homo sapiens
10 PRPS2 5634
Affinity Capture-MS Homo sapiens
11 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 NPRL3  
Affinity Capture-MS Homo sapiens
13 TPD52L2 7165
Co-fractionation Homo sapiens
14 NFYC 4802
Proximity Label-MS Homo sapiens
15 RIT1 6016
Negative Genetic Homo sapiens
16 PFN1 5216
Proximity Label-MS Homo sapiens
17 CALU 813
Co-fractionation Homo sapiens
18 SOX12  
Affinity Capture-MS Homo sapiens
19 TRIM66  
Affinity Capture-MS Homo sapiens
20 DNAJB2 3300
Proximity Label-MS Homo sapiens
21 ARRDC4  
Affinity Capture-MS Homo sapiens
22 RAD51B  
Affinity Capture-MS Homo sapiens
23 F12  
Affinity Capture-MS Homo sapiens
24 EMILIN1 11117
Affinity Capture-MS Homo sapiens
25 CDK18 5129
Affinity Capture-MS Homo sapiens
26 CUL3 8452
Affinity Capture-MS Homo sapiens
27 ARPC2 10109
Co-fractionation Homo sapiens
28 LSM4 25804
Co-fractionation Homo sapiens
29 UBC 7316
Affinity Capture-MS Homo sapiens
30 AKAP13 11214
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
32 LRRC27  
Affinity Capture-MS Homo sapiens
33 IQCF1  
Affinity Capture-MS Homo sapiens
34 RIPK4  
Affinity Capture-MS Homo sapiens
35 LPCAT1 79888
Affinity Capture-MS Homo sapiens
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 ILF2 3608
Co-fractionation Homo sapiens
38 ANAPC2 29882
Proximity Label-MS Homo sapiens
39 BRD1 23774
Affinity Capture-MS Homo sapiens
40 C6orf141  
Affinity Capture-MS Homo sapiens
41 RCCD1  
Affinity Capture-MS Homo sapiens
42 C12orf74  
Affinity Capture-MS Homo sapiens
43 DHX16 8449
Co-fractionation Homo sapiens
44 TSC22D1 8848
Affinity Capture-MS Homo sapiens
45 INF2 64423
Affinity Capture-MS Homo sapiens
46 SOHLH1  
Affinity Capture-MS Homo sapiens
47 ARL4D  
Affinity Capture-MS Homo sapiens
48 VWA2 340706
Affinity Capture-MS Homo sapiens
49 NTRK1 4914
Affinity Capture-MS Homo sapiens
50 TFDP3  
Affinity Capture-MS Homo sapiens
51 METTL21B  
Affinity Capture-MS Homo sapiens
52 SQSTM1 8878
Proximity Label-MS Homo sapiens
53 ALDH3B1 221
Affinity Capture-MS Homo sapiens
54 MOV10 4343
Affinity Capture-RNA Homo sapiens
55 HUWE1 10075
Affinity Capture-MS Homo sapiens
56 TXNIP 10628
Affinity Capture-MS Homo sapiens
57 THG1L 54974
Co-fractionation Homo sapiens
58 DHFRL1  
Proximity Label-MS Homo sapiens
59 C9orf72  
Affinity Capture-MS Homo sapiens
60 XRCC3  
Affinity Capture-MS Homo sapiens
61 HAX1  
Proximity Label-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 NSUN5P1  
Affinity Capture-MS Homo sapiens
64 NAPG 8774
Co-fractionation Homo sapiens
65 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
66 CDKN2A 1029
Reconstituted Complex Homo sapiens
67 UBXN6 80700
Affinity Capture-MS Homo sapiens
68 ARPC1A 10552
Co-fractionation Homo sapiens
69 KLHL14 57565
Affinity Capture-MS Homo sapiens
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