Gene description for Lck
Gene name lymphocyte protein tyrosine kinase
Gene symbol Lck
Other names/aliases Hck-3
Lsk
Lskt
p56
p56Lck
Species Mus musculus
 Database cross references - Lck
ExoCarta ExoCarta_16818
Vesiclepedia VP_16818
Entrez Gene 16818
UniProt P06240  
 Lck identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lck
Molecular Function
    phosphotyrosine residue binding GO:0001784 ISO
    antigen binding GO:0003823 ISO
    protein tyrosine kinase activity GO:0004713 EXP
    protein tyrosine kinase activity GO:0004713 IDA
    protein tyrosine kinase activity GO:0004713 IMP
    protein tyrosine kinase activity GO:0004713 ISO
    protein tyrosine kinase activity GO:0004713 ISS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 ISO
    protein serine/threonine phosphatase activity GO:0004722 ISO
    protein serine/threonine phosphatase activity GO:0004722 ISS
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    phospholipase activator activity GO:0016004 ISO
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 IPI
    protein phosphatase binding GO:0019903 ISO
    histone H3Y41 kinase activity GO:0035401 IEA
    SH2 domain binding GO:0042169 ISO
    SH2 domain binding GO:0042169 ISS
    T cell receptor binding GO:0042608 ISO
    CD4 receptor binding GO:0042609 IPI
    CD4 receptor binding GO:0042609 ISO
    CD8 receptor binding GO:0042610 ISO
    identical protein binding GO:0042802 ISO
    phospholipase binding GO:0043274 ISO
    phosphatidylinositol 3-kinase binding GO:0043548 ISO
    protein-containing complex binding GO:0044877 ISO
    ATPase binding GO:0051117 ISO
    histone H2AXY142 kinase activity GO:0140801 IEA
    protein antigen binding GO:1990405 IPI
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    intracellular zinc ion homeostasis GO:0006882 ISS
    cell surface receptor signaling pathway GO:0007166 IDA
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    response to xenobiotic stimulus GO:0009410 ISO
    response to xenobiotic stimulus GO:0009410 ISS
    response to mechanical stimulus GO:0009612 ISO
    response to metal ion GO:0010038 ISO
    positive regulation of gene expression GO:0010628 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 ISO
    T cell differentiation GO:0030217 IBA
    T cell differentiation GO:0030217 ISO
    T cell differentiation GO:0030217 ISS
    positive regulation of heterotypic cell-cell adhesion GO:0034116 ISO
    intracellular signal transduction GO:0035556 ISO
    peptidyl-tyrosine autophosphorylation GO:0038083 ISO
    Fc-gamma receptor signaling pathway GO:0038094 ISO
    gamma-delta T cell differentiation GO:0042492 IMP
    positive regulation of tyrosine phosphorylation of STAT protein GO:0042531 IDA
    response to hydrogen peroxide GO:0042542 ISO
    positive regulation of gamma-delta T cell differentiation GO:0045588 IMP
    regulation of regulatory T cell differentiation GO:0045589 IGI
    protein autophosphorylation GO:0046777 IDA
    T cell receptor signaling pathway GO:0050852 ISO
    B cell receptor signaling pathway GO:0050853 IBA
    B cell receptor signaling pathway GO:0050853 IDA
    regulation of T cell receptor signaling pathway GO:0050856 IDA
    positive regulation of T cell activation GO:0050870 ISO
    positive regulation of T cell activation GO:0050870 ISS
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    positive regulation of uterine smooth muscle contraction GO:0070474 ISO
    positive regulation of leukocyte cell-cell adhesion GO:1903039 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISS
Subcellular Localization
    pericentriolar material GO:0000242 ISO
    pericentriolar material GO:0000242 ISS
    immunological synapse GO:0001772 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell-cell junction GO:0005911 IDA
    endocytic vesicle GO:0030139 ISO
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISS
    glutamatergic synapse GO:0098978 ISO
    postsynaptic specialization, intracellular component GO:0099091 ISO
 Experiment description of studies that identified Lck in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lck
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cd38 12494
Affinity Capture-Western Mus musculus
2 LCK 3932
Affinity Capture-MS Homo sapiens
3 Cyld  
Affinity Capture-Western Mus musculus
4 ATP5I 521
Affinity Capture-MS Homo sapiens
5 Pik3r1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
6 MTNR1B  
Co-purification Homo sapiens
7 NSMCE2  
Affinity Capture-MS Homo sapiens
8 AES 166
Affinity Capture-MS Homo sapiens
9 Stap1  
Two-hybrid Mus musculus
10 MEN1 4221
Affinity Capture-MS Homo sapiens
11 HPS5 11234
Affinity Capture-MS Homo sapiens
12 APOE 348
Affinity Capture-MS Homo sapiens
13 CAMSAP2  
Affinity Capture-MS Homo sapiens
14 Dlg1 13383
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
15 Socs6  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
16 Cblb 208650
Biochemical Activity Mus musculus
17 NELFCD 51497
Affinity Capture-MS Homo sapiens
18 Cd4  
Affinity Capture-Western Mus musculus
19 ESCO1  
Affinity Capture-MS Homo sapiens
20 CDK13 8621
Affinity Capture-MS Homo sapiens
21 PHF5A 84844
Affinity Capture-MS Homo sapiens
22 ZBED8  
Affinity Capture-MS Homo sapiens
23 YES1 7525
Affinity Capture-MS Homo sapiens
24 C2CD5 9847
Affinity Capture-MS Homo sapiens
25 SPAST 6683
Affinity Capture-MS Homo sapiens
26 Zap70 22637
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
27 Cbl 12402
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
28 Fancd2  
Affinity Capture-MS Mus musculus
29 Was 22376
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
View the network image/svg+xml
 Pathways in which Lck is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
CD28 co-stimulation IEA Reactome
CD28 dependent PI3K/Akt signaling IEA Reactome
CD28 dependent Vav1 pathway IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Costimulation by the CD28 family IEA Reactome
CTLA4 inhibitory signaling IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DAP12 interactions IEA Reactome
DAP12 signaling IEA Reactome
Downstream TCR signaling IEA Reactome
FLT3 Signaling IEA Reactome
FLT3 signaling through SRC family kinases IEA Reactome
Generation of second messenger molecules IEA Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Interleukin-2 family signaling IEA Reactome
Interleukin-2 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
PD-1 signaling IEA Reactome
PECAM1 interactions IEA Reactome
Phosphorylation of CD3 and TCR zeta chains IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Regulation of KIT signaling IEA Reactome
RHO GTPase cycle IEA Reactome
RHOH GTPase cycle IEA Reactome
Signal Transduction IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by SCF-KIT IEA Reactome
TCR signaling IEA Reactome
Translocation of ZAP-70 to Immunological synapse IEA Reactome





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