Gene description for STX1A
Gene name syntaxin 1A (brain)
Gene symbol STX1A
Other names/aliases HPC-1
P35-1
STX1
SYN1A
Species Homo sapiens
 Database cross references - STX1A
ExoCarta ExoCarta_6804
Entrez Gene 6804
HGNC 11433
MIM 186590
UniProt Q16623  
 STX1A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for STX1A
Molecular Function
    protein binding GO:0005515 IPI
    protein N-terminus binding GO:0047485 IEA
    calcium channel inhibitor activity GO:0019855 IEA
    protein binding, bridging GO:0030674 IEA
    kinase binding GO:0019900 IPI
    glycoprotein binding GO:0001948 IEA
    ATP-dependent protein binding GO:0043008 IEA
    calcium-dependent protein binding GO:0048306 IEA
    chloride channel inhibitor activity GO:0019869 IDA
    protein domain specific binding GO:0019904 IEA
    SNARE binding GO:0000149 IBA
    protein heterodimerization activity GO:0046982 IEA
    SNAP receptor activity GO:0005484 IBA
    myosin head/neck binding GO:0032028 IEA
    myosin binding GO:0017022 IBA
Biological Process
    regulation of insulin secretion GO:0050796 TAS
    synaptic vesicle docking GO:0016081 IEA
    small molecule metabolic process GO:0044281 TAS
    positive regulation of calcium ion-dependent exocytosis GO:0045956 ISS
    exocytosis GO:0006887 IBA
    regulation of synaptic vesicle priming GO:0010807 IEA
    glutamate secretion GO:0014047 TAS
    intracellular protein transport GO:0006886 IBA
    energy reserve metabolic process GO:0006112 TAS
    positive regulation of norepinephrine secretion GO:0010701 ISS
    vesicle docking GO:0048278 IBA
    synaptic vesicle fusion to presynaptic membrane GO:0031629 IBA
    positive regulation of neurotransmitter secretion GO:0001956 IEA
    response to gravity GO:0009629 IEA
    cellular protein metabolic process GO:0044267 TAS
    calcium ion-dependent exocytosis GO:0017156 IEA
    synaptic transmission GO:0007268 TAS
    positive regulation of excitatory postsynaptic membrane potential GO:2000463 IEA
    positive regulation of catecholamine secretion GO:0033605 ISS
    neurotransmitter secretion GO:0007269 TAS
    secretion by cell GO:0032940 IDA
Subcellular Localization
    secretory granule GO:0030141 IEA
    synaptic vesicle GO:0008021 IBA
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
    extracellular region GO:0005576 IEA
    synaptobrevin 2-SNAP-25-syntaxin-1a complex GO:0070044 IEA
    cell junction GO:0030054 IEA
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex GO:0070032 IEA
    synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex GO:0070033 IEA
    actomyosin GO:0042641 IEA
    SNARE complex GO:0031201 TAS
    integral component of membrane GO:0016021 IBA
    neuron projection GO:0043005 IDA
    synaptic vesicle membrane GO:0030672 IEA
    endomembrane system GO:0012505 IBA
    voltage-gated potassium channel complex GO:0008076 ISS
 Experiment description of studies that identified STX1A in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for STX1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TXLNA 200081
Invitro Homo sapiens
2 SNAP23 8773
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
3 SCNN1G  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 SYTL4 94121
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 TRDMT1  
Two-hybrid Homo sapiens
6 SCNN1A  
Affinity Capture-Western Homo sapiens
7 RNF40 9810
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 STXBP5 134957
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
9 VAPB 9217
Reconstituted Complex Homo sapiens
10 NSF 4905
Invivo Homo sapiens
Invitro Homo sapiens
11 VAMP8 8673
Invitro Homo sapiens
Invivo Homo sapiens
12 NAPA 8775
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
13 STXBP6 29091
Invitro Homo sapiens
Invivo Homo sapiens
14 STXBP1 6812
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 SEPT5 5413
Invivo Homo sapiens
Invitro Homo sapiens
16 C6orf198  
Reconstituted Complex Homo sapiens
17 SNAP25 6616
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 VAMP2 6844
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
19 SYT4  
Invitro Homo sapiens
20 CDK5 1020
Invitro Homo sapiens
21 SLC6A9 6536
Invivo Homo sapiens
22 SLC6A4 6532
Affinity Capture-Western Homo sapiens
23 SEPT2 4735
Invitro Homo sapiens
Invivo Homo sapiens
24 KIAA1128  
Two-hybrid Homo sapiens
25 SLC6A1  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
26 SCNN1B  
Affinity Capture-Western Homo sapiens
27 SYP  
Affinity Capture-Western Homo sapiens
28 VAMP7 6845
Reconstituted Complex Homo sapiens
29 CFTR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
30 CPLX2 10814
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
31 STXBP2 6813
Reconstituted Complex Homo sapiens
32 Snap25  
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
33 CACNA1D  
Invivo Homo sapiens
34 VAMP1 6843
Two-hybrid Homo sapiens
35 ATP6V1B1 525
Reconstituted Complex Homo sapiens
36 Snap23  
Two-hybrid Mus musculus
Two-hybrid Mus musculus
37 VIM 7431
Two-hybrid Homo sapiens
38 UNC13B 10497
Invivo Homo sapiens
Two-hybrid Homo sapiens
39 SYT1 6857
Reconstituted Complex Homo sapiens
40 CPLX1 10815
Affinity Capture-Western Homo sapiens
41 SNAP29 9342
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here