Gene description for STX2
Gene name syntaxin 2
Gene symbol STX2
Other names/aliases EPIM
EPM
STX2A
STX2B
STX2C
Species Homo sapiens
 Database cross references - STX2
ExoCarta ExoCarta_2054
Vesiclepedia VP_2054
Entrez Gene 2054
HGNC 3403
MIM 132350
UniProt P32856  
 STX2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for STX2
Molecular Function
    SNARE binding GO:0000149 IBA
    structural molecule activity GO:0005198 IDA
    SNAP receptor activity GO:0005484 IBA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    calcium-dependent protein binding GO:0048306 ISS
Biological Process
    intracellular protein transport GO:0006886 IBA
    exocytosis GO:0006887 IBA
    signal transduction GO:0007165 TAS
    acrosome reaction GO:0007340 ISS
    ectoderm development GO:0007398 TAS
    animal organ morphogenesis GO:0009887 TAS
    synaptic vesicle fusion to presynaptic active zone membrane GO:0031629 IBA
    cellular response to oxidative stress GO:0034599 IDA
    vesicle docking GO:0048278 IBA
    cornified envelope assembly GO:1903575 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    endomembrane system GO:0012505 IBA
    basolateral plasma membrane GO:0016323 IDA
    lamellipodium GO:0030027 IDA
    SNARE complex GO:0031201 IBA
    protein-containing complex GO:0032991 IDA
    intracellular membrane-bounded organelle GO:0043231 TAS
    presynaptic active zone membrane GO:0048787 IBA
 Experiment description of studies that identified STX2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for STX2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Two-hybrid Homo sapiens
2 STX6 10228
Two-hybrid Homo sapiens
3 BLOC1S2 282991
Two-hybrid Homo sapiens
4 BET1 10282
Two-hybrid Homo sapiens
5 SYTL4 94121
Affinity Capture-MS Homo sapiens
6 NINJ2  
Two-hybrid Homo sapiens
7 ABI3 51225
Two-hybrid Homo sapiens
8 ZNF785  
Two-hybrid Homo sapiens
9 Snap23 20619
Two-hybrid Mus musculus
Two-hybrid Mus musculus
10 VTI1B 10490
Two-hybrid Homo sapiens
11 VAPB 9217
Reconstituted Complex Homo sapiens
12 STX12 23673
Two-hybrid Homo sapiens
13 SNAP23 8773
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
14 TP53 7157
Synthetic Growth Defect Homo sapiens
15 VAMP8 8673
Affinity Capture-Western Homo sapiens
16 ZFPL1 7542
Two-hybrid Homo sapiens
17 STXBP5L  
Two-hybrid Homo sapiens
18 RTP2  
Two-hybrid Homo sapiens
19 MIP  
Two-hybrid Homo sapiens
20 CD53 963
Two-hybrid Homo sapiens
21 STXBP1 6812
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
22 LOC100507537  
Two-hybrid Homo sapiens
23 TMEM120B  
Two-hybrid Homo sapiens
24 VAMP3 9341
Two-hybrid Homo sapiens
25 PPAPDC1A  
Two-hybrid Homo sapiens
26 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
27 VAMP2 6844
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 PLN  
Two-hybrid Homo sapiens
29 FGA 2243
Two-hybrid Homo sapiens
30 SNAP25 6616
Reconstituted Complex Homo sapiens
31 STX4 6810
Two-hybrid Homo sapiens
32 NAPB 63908
Two-hybrid Homo sapiens
33 NXF1 10482
Affinity Capture-RNA Homo sapiens
34 PCGF2 7703
Two-hybrid Homo sapiens
35 ENTPD3  
Two-hybrid Homo sapiens
36 UBE2I 7329
Two-hybrid Homo sapiens
37 TSPAN8 7103
Two-hybrid Homo sapiens
38 MOV10 4343
Affinity Capture-RNA Homo sapiens
39 EBAG9 9166
Two-hybrid Homo sapiens
40 STX1A 6804
Two-hybrid Homo sapiens
41 VAMP5 10791
Two-hybrid Homo sapiens
42 WRB 7485
Two-hybrid Homo sapiens
43 STX3 6809
Two-hybrid Homo sapiens
44 STXBP2 6813
Affinity Capture-MS Homo sapiens
45 AARD  
Two-hybrid Homo sapiens
46 SEC22A  
Two-hybrid Homo sapiens
47 Snap25  
Two-hybrid Rattus norvegicus
Two-hybrid Rattus norvegicus
48 MTG1  
Two-hybrid Homo sapiens
49 COL4A3BP 10087
Two-hybrid Homo sapiens
50 STX8 9482
Two-hybrid Homo sapiens
51 ENO1 2023
Affinity Capture-RNA Homo sapiens
52 CERS6  
Affinity Capture-MS Homo sapiens
53 STX16 8675
Two-hybrid Homo sapiens
54 STX5 6811
Two-hybrid Homo sapiens
55 APOL2 23780
Two-hybrid Homo sapiens
56 MRPL11 65003
Two-hybrid Homo sapiens
57 ANKRD46 157567
Two-hybrid Homo sapiens
58 SERP1 27230
Two-hybrid Homo sapiens
59 TMEM120A 83862
Two-hybrid Homo sapiens
60 C17orf62 79415
Two-hybrid Homo sapiens
61 NSF 4905
Affinity Capture-MS Homo sapiens
62 GIMAP5  
Two-hybrid Homo sapiens
63 ZCCHC12  
Two-hybrid Homo sapiens
64 SYT1 6857
Reconstituted Complex Homo sapiens
65 RMDN3 55177
Two-hybrid Homo sapiens
66 STX2 2054
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
67 STXBP3 6814
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which STX2 is involved
No pathways found





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