Gene description for Snap23
Gene name synaptosomal-associated protein 23
Gene symbol Snap23
Other names/aliases 23kDa
AA408749
SNAP-23
Sndt
Syndet
Species Mus musculus
 Database cross references - Snap23
ExoCarta ExoCarta_20619
Vesiclepedia VP_20619
Entrez Gene 20619
UniProt O09044  
 Snap23 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Snap23
Molecular Function
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 ISO
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IBA
    syntaxin binding GO:0019905 ISO
Biological Process
    histamine secretion by mast cell GO:0002553 ISO
    exocytosis GO:0006887 IBA
    exocytosis GO:0006887 IMP
    protein transport GO:0015031 IEA
    synaptic vesicle priming GO:0016082 IBA
    vesicle-mediated transport GO:0016192 ISO
    regulation of exocytosis GO:0017157 ISO
    synaptic vesicle fusion to presynaptic active zone membrane GO:0031629 IBA
    membrane fusion GO:0061025 ISO
    protein-containing complex assembly GO:0065003 ISO
    exocytic insertion of neurotransmitter receptor to postsynaptic membrane GO:0098967 ISO
    vesicle-mediated transport in synapse GO:0099003 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    adherens junction GO:0005912 ISO
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 IDA
    SNARE complex GO:0031201 ISO
    cytoplasmic vesicle GO:0031410 IDA
    specific granule GO:0042581 ISO
    azurophil granule GO:0042582 ISO
    neuron projection GO:0043005 IEA
    synapse GO:0045202 IEA
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 ISO
 Experiment description of studies that identified Snap23 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Snap23
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vamp2  
Affinity Capture-Western Mus musculus
2 Snap29  
Co-fractionation Mus musculus
3 Chuk  
Affinity Capture-Western Mus musculus
4 Stx1a  
Affinity Capture-Western Mus musculus
5 STX1A 6804
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 Snrnp200 320632
Co-fractionation Mus musculus
7 IKBKB 3551
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 Ikbkb 16150
Affinity Capture-Western Mus musculus
9 STX3 6809
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 Stx4a 20909
Co-fractionation Mus musculus
11 Chchd4  
Co-fractionation Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
13 STX4 6810
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 STX2 2054
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here