Gene description for FXYD3
Gene name FXYD domain containing ion transport regulator 3
Gene symbol FXYD3
Other names/aliases MAT8
PLML
Species Homo sapiens
 Database cross references - FXYD3
ExoCarta ExoCarta_5349
Entrez Gene 5349
HGNC 4027
MIM 604996
UniProt Q14802  
 FXYD3 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Saliva 19199708    
 Gene ontology annotations for FXYD3
Molecular Function
    chloride channel activity GO:0005254 IEA
    ATPase binding GO:0051117 IEA
    sodium channel regulator activity GO:0017080 IBA
Biological Process
    chloride transmembrane transport GO:1902476 TAS
    chloride transport GO:0006821 TAS
    regulation of catalytic activity GO:0050790 IEA
    regulation of sodium ion transmembrane transporter activity GO:2000649 IBA
    ion transmembrane transport GO:0034220 TAS
    transmembrane transport GO:0055085 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    integral component of plasma membrane GO:0005887 TAS
    chloride channel complex GO:0034707 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified FXYD3 in exosomes
1
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for FXYD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXL12 54850
Two-hybrid Homo sapiens
2 SERBP1 26135
Two-hybrid Homo sapiens
3 NR4A1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which FXYD3 is involved
PathwayEvidenceSource
Ion transport by P-type ATPases TAS Reactome





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