Gene description for FXYD3
Gene name FXYD domain containing ion transport regulator 3
Gene symbol FXYD3
Other names/aliases MAT8
PLML
Species Homo sapiens
 Database cross references - FXYD3
ExoCarta ExoCarta_5349
Vesiclepedia VP_5349
Entrez Gene 5349
HGNC 4027
MIM 604996
UniProt Q14802  
 FXYD3 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Saliva 19199708    
 Gene ontology annotations for FXYD3
Molecular Function
    chloride channel activity GO:0005254 TAS
    protein binding GO:0005515 IPI
    sodium channel regulator activity GO:0017080 IBA
    ATPase binding GO:0051117 IEA
Biological Process
    potassium ion transport GO:0006813 IEA
    sodium ion transport GO:0006814 IEA
    chloride transport GO:0006821 TAS
    chloride transmembrane transport GO:1902476 IEA
    positive regulation of sodium ion export across plasma membrane GO:1903278 IBA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified FXYD3 in exosomes
1
Experiment ID 258
MISEV standards
EM
Biophysical techniques
TSG101|FLOT1|CD81
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for FXYD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1H 51606
Affinity Capture-MS Homo sapiens
2 ATR  
Affinity Capture-MS Homo sapiens
3 NR4A1  
Two-hybrid Homo sapiens
4 GLMN 11146
Affinity Capture-MS Homo sapiens
5 USP22 23326
Affinity Capture-MS Homo sapiens
6 EXOC2 55770
Affinity Capture-MS Homo sapiens
7 ARFGEF1 10565
Affinity Capture-MS Homo sapiens
8 DNAAF5 54919
Affinity Capture-MS Homo sapiens
9 GEMIN8 54960
Affinity Capture-MS Homo sapiens
10 TNPO3 23534
Affinity Capture-MS Homo sapiens
11 TNPO1 3842
Affinity Capture-MS Homo sapiens
12 GBF1 8729
Affinity Capture-MS Homo sapiens
13 NDRG3 57446
Affinity Capture-MS Homo sapiens
14 CREB3L1  
Two-hybrid Homo sapiens
15 FBXL12 54850
Two-hybrid Homo sapiens
16 NDRG4 65009
Affinity Capture-MS Homo sapiens
17 XPO6 23214
Affinity Capture-MS Homo sapiens
18 DCUN1D5 84259
Affinity Capture-MS Homo sapiens
19 MAPK6  
Two-hybrid Homo sapiens
20 XPO5 57510
Affinity Capture-MS Homo sapiens
21 TNPO2 30000
Affinity Capture-MS Homo sapiens
22 CREB3  
Two-hybrid Homo sapiens
23 PDS5A 23244
Affinity Capture-MS Homo sapiens
24 NDRG1 10397
Affinity Capture-MS Homo sapiens
25 DNM3 26052
Affinity Capture-MS Homo sapiens
26 KIAA1524 57650
Affinity Capture-MS Homo sapiens
27 BRAT1 221927
Affinity Capture-MS Homo sapiens
28 NR2F2  
Affinity Capture-MS Homo sapiens
29 SERBP1 26135
Two-hybrid Homo sapiens
30 NUDT3 11165
Two-hybrid Homo sapiens
31 TKFC 26007
Affinity Capture-MS Homo sapiens
32 THADA 63892
Affinity Capture-MS Homo sapiens
33 LEMD3  
Affinity Capture-MS Homo sapiens
34 XPO7 23039
Affinity Capture-MS Homo sapiens
35 TBC1D15 64786
Affinity Capture-MS Homo sapiens
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