Gene description for POU2F1
Gene name POU class 2 homeobox 1
Gene symbol POU2F1
Other names/aliases OCT1
OTF1
oct-1B
Species Homo sapiens
 Database cross references - POU2F1
ExoCarta ExoCarta_5451
Vesiclepedia VP_5451
Entrez Gene 5451
HGNC 9212
MIM 164175
UniProt P14859  
 POU2F1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for POU2F1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IMP
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IMP
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IMP
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IC
    DNA binding GO:0003677 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    sequence-specific DNA binding GO:0043565 IDA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of miRNA transcription GO:1902895 IC
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    endoplasmic reticulum GO:0005783 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    RNA polymerase II transcription regulator complex GO:0090575 IMP
 Experiment description of studies that identified POU2F1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for POU2F1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
Affinity Capture-MS Homo sapiens
2 ZFHX3  
Two-hybrid Homo sapiens
3 FEV  
Proximity Label-MS Homo sapiens
4 STAT5B 6777
Affinity Capture-Western Homo sapiens
5 RPS4X 6191
Affinity Capture-MS Homo sapiens
6 CMTM8  
Affinity Capture-MS Homo sapiens
7 SP1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
8 RPL37A 6168
Affinity Capture-MS Homo sapiens
9 SOX17  
Proximity Label-MS Homo sapiens
10 BRCA1 672
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 PAX2  
Proximity Label-MS Homo sapiens
12 RPS19 6223
Affinity Capture-MS Homo sapiens
13 FDXR 2232
Affinity Capture-MS Homo sapiens
14 PPIA 5478
Affinity Capture-MS Homo sapiens
15 PGR  
Reconstituted Complex Homo sapiens
16 PRKDC 5591
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
17 APP 351
Reconstituted Complex Homo sapiens
18 HSPA5 3309
Affinity Capture-MS Homo sapiens
19 NFKB1 4790
Co-fractionation Homo sapiens
20 HSPA2 3306
Affinity Capture-MS Homo sapiens
21 KLF8  
Proximity Label-MS Homo sapiens
22 CDK11B 984
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 STOX1  
Two-hybrid Homo sapiens
24 PRR13  
Affinity Capture-MS Homo sapiens
25 IRF9 10379
Two-hybrid Homo sapiens
26 STAT3 6774
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
27 MAP4K5 11183
Co-fractionation Homo sapiens
28 HMGA1 3159
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
29 STIP1 10963
Affinity Capture-Western Homo sapiens
30 PKNOX1  
Reconstituted Complex Homo sapiens
31 VDR  
Reconstituted Complex Homo sapiens
32 SOX5 6660
Proximity Label-MS Homo sapiens
33 PRR20C  
Two-hybrid Homo sapiens
34 EEF1A1 1915
Affinity Capture-MS Homo sapiens
35 ALOXE3  
Affinity Capture-MS Homo sapiens
36 HSPA8 3312
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TNF  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
38 KLF16  
Proximity Label-MS Homo sapiens
39 KRT8 3856
Affinity Capture-MS Homo sapiens
40 EAF2  
Affinity Capture-MS Homo sapiens
41 S100A2 6273
Affinity Capture-MS Homo sapiens
42 PBX1 5087
Reconstituted Complex Homo sapiens
43 FOXC1  
Affinity Capture-MS Homo sapiens
44 PKM 5315
Affinity Capture-MS Homo sapiens
45 TLX3  
Proximity Label-MS Homo sapiens
46 MAGEA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PGAM5 192111
Affinity Capture-MS Homo sapiens
48 SNAPC4  
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
49 NCOR2  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
50 NFIX 4784
Proximity Label-MS Homo sapiens
51 ATF2  
Affinity Capture-MS Homo sapiens
52 CEBPE  
Two-hybrid Homo sapiens
53 UBE2I 7329
Two-hybrid Homo sapiens
54 POU6F2  
Two-hybrid Homo sapiens
55 SNIP1  
Two-hybrid Homo sapiens
56 TUBA1B 10376
Affinity Capture-MS Homo sapiens
57 MNAT1  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
58 POGZ 23126
Two-hybrid Homo sapiens
59 TRIM21 6737
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
60 RPL31 6160
Affinity Capture-MS Homo sapiens
61 FOXC2  
Affinity Capture-MS Homo sapiens
62 TEAD2  
Affinity Capture-MS Homo sapiens
63 BANF1 8815
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 GPC5 2262
Affinity Capture-MS Homo sapiens
65 TGM1 7051
Affinity Capture-MS Homo sapiens
66 PLK3  
Two-hybrid Homo sapiens
67 CEBPA  
Proximity Label-MS Homo sapiens
68 FOXS1  
Affinity Capture-MS Homo sapiens
69 HIST1H2AB 8335
Affinity Capture-MS Homo sapiens
70 BRD7  
Co-localization Homo sapiens
71 TLX2  
Two-hybrid Homo sapiens
72 PAX6  
Proximity Label-MS Homo sapiens
73 IPO5 3843
Affinity Capture-MS Homo sapiens
74 SOX6  
Proximity Label-MS Homo sapiens
75 LMNB1 4001
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 BAG2 9532
Affinity Capture-MS Homo sapiens
77 RELA 5970
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
78 ELK3  
Proximity Label-MS Homo sapiens
79 PRR20D  
Two-hybrid Homo sapiens
80 HCFC1 3054
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
81 NR3C1 2908
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
82 TXNRD1 7296
Reconstituted Complex Homo sapiens
83 HSD17B10 3028
Affinity Capture-MS Homo sapiens
84 FOXL1  
Proximity Label-MS Homo sapiens
85 SPTBN1 6711
Co-fractionation Homo sapiens
86 ZC3H10  
Two-hybrid Homo sapiens
87 THRA  
Reconstituted Complex Homo sapiens
88 TBP  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
89 NT5C2 22978
Affinity Capture-MS Homo sapiens
90 PRMT5 10419
Affinity Capture-MS Homo sapiens
91 PRKACA 5566
Biochemical Activity Homo sapiens
92 PRR20B  
Two-hybrid Homo sapiens
93 ZMYM6  
Two-hybrid Homo sapiens
94 BAG1 573
Affinity Capture-MS Homo sapiens
95 PPIP5K2 23262
Two-hybrid Homo sapiens
96 RPS14 6208
Affinity Capture-MS Homo sapiens
97 HOXC4  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
98 EEF1A2 1917
Affinity Capture-MS Homo sapiens
99 HSPA4 3308
Affinity Capture-MS Homo sapiens
100 ACY1 95
Co-fractionation Homo sapiens
101 PASK  
Affinity Capture-MS Homo sapiens
102 XRCC5 7520
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
103 SOX2  
Proximity Label-MS Homo sapiens
104 PROSC 11212
Co-fractionation Homo sapiens
105 ARPC3 10094
Two-hybrid Homo sapiens
106 ARRB2 409
Affinity Capture-MS Homo sapiens
107 FASN 2194
Positive Genetic Homo sapiens
108 RARA 5914
Reconstituted Complex Homo sapiens
109 KCTD17 79734
Affinity Capture-MS Homo sapiens
110 PRR20A  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
111 SCYL2 55681
Affinity Capture-MS Homo sapiens
112 SOX15  
Proximity Label-MS Homo sapiens
113 EPCAM 4072
Affinity Capture-MS Homo sapiens
114 POU2AF1  
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
115 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 NPAT  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
117 HDAC1 3065
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
118 Pbx1  
Two-hybrid Mus musculus
119 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
120 CREB1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
121 HNRNPK 3190
Affinity Capture-MS Homo sapiens
122 ECHDC1 55862
Co-fractionation Homo sapiens
123 POU2F1 5451
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
124 ESR1  
Reconstituted Complex Homo sapiens
125 SP4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
126 HOXB13  
Two-hybrid Homo sapiens
127 PAX7  
Proximity Label-MS Homo sapiens
128 HOXC9  
Two-hybrid Homo sapiens
129 RPS24 6229
Affinity Capture-MS Homo sapiens
130 GAPDH 2597
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
131 PRR20E  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
132 ARRB1 408
Two-hybrid Homo sapiens
133 ATP5B 506
Affinity Capture-MS Homo sapiens
134 RXRA 6256
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
135 XRCC6 2547
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
136 ELF2  
Proximity Label-MS Homo sapiens
137 HNRNPU 3192
Affinity Capture-MS Homo sapiens
138 NFIC 4782
Proximity Label-MS Homo sapiens
139 AR 367
Reconstituted Complex Homo sapiens
140 RPL22L1 200916
Affinity Capture-MS Homo sapiens
141 SP7  
Proximity Label-MS Homo sapiens
142 DARS2 55157
Co-fractionation Homo sapiens
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