Gene ontology annotations for GOLPH3
Experiment description of studies that identified GOLPH3 in exosomes
1
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
209
MISEV standards
✘
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
8
Experiment ID
1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
9
Experiment ID
235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
10
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
11
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
12
Experiment ID
191
MISEV standards
✘
Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20124223
Organism
Homo sapiens
Experiment description
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors
"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name
Mol Cell Proteomics
Publication year
2010
Sample
Squamous carcinoma cells
Sample name
Squamous carcinoma cell (A431)
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for GOLPH3
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
COPG2
26958
Affinity Capture-MS
Homo sapiens
2
VPS35
55737
Affinity Capture-Western
Homo sapiens
3
C5AR2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
4
C5AR1
Affinity Capture-MS
Homo sapiens
5
RASSF8
11228
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
6
ADCK3
Affinity Capture-MS
Homo sapiens
7
C3orf58
205428
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
8
RAMP1
Affinity Capture-MS
Homo sapiens
9
TSR3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
10
MAN1B1
11253
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
11
TNFRSF8
943
Affinity Capture-MS
Homo sapiens
12
ARCN1
372
Affinity Capture-MS
Homo sapiens
13
NDUFA4
4697
Affinity Capture-MS
Homo sapiens
14
CAP2
10486
Co-fractionation
Homo sapiens
15
ARL6IP1
23204
Two-hybrid
Homo sapiens
16
COPA
1314
Affinity Capture-MS
Homo sapiens
17
PNKD
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
18
FAM20B
9917
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
19
B4GALT4
8702
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
20
GLG1
2734
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
21
COQ9
Affinity Capture-MS
Homo sapiens
22
COPB2
9276
Affinity Capture-MS
Homo sapiens
23
WBSCR16
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
24
AP1G1
164
Co-fractionation
Homo sapiens
25
PFDN4
5203
Co-fractionation
Homo sapiens
26
MDC1
Affinity Capture-MS
Homo sapiens
27
AMIGO3
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
28
NEDD8-MDP1
100528064
Affinity Capture-MS
Homo sapiens
29
SNX27
81609
Affinity Capture-MS
Homo sapiens
30
GPRC5C
55890
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
31
ADCK4
79934
Affinity Capture-MS
Homo sapiens
32
KIF14
9928
Affinity Capture-MS
Homo sapiens
33
VTI1A
143187
Affinity Capture-MS
Homo sapiens
34
RB1
5925
Co-fractionation
Homo sapiens
35
GMPPA
29926
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
36
GLT8D1
55830
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
37
S100A2
6273
Affinity Capture-MS
Homo sapiens
38
POMGNT1
55624
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
39
CANT1
124583
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
40
FAM3C
10447
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
41
GCNT1
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
42
ST3GAL1
6482
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
43
COPE
11316
Affinity Capture-MS
Homo sapiens
44
ACADVL
37
Co-fractionation
Homo sapiens
45
P2RY8
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
46
COPG1
22820
Affinity Capture-MS
Homo sapiens
47
COPB1
1315
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
48
KRTCAP3
200634
Affinity Capture-MS
Homo sapiens
49
AVPR2
Affinity Capture-MS
Homo sapiens
50
SEC61B
10952
Affinity Capture-MS
Homo sapiens
51
FAM69A
388650
Affinity Capture-MS
Homo sapiens
52
ACN9
Affinity Capture-MS
Homo sapiens
53
MLNR
Affinity Capture-MS
Homo sapiens
54
C15orf48
Affinity Capture-MS
Homo sapiens
55
CASC4
113201
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
56
TMEM106B
54664
Affinity Capture-MS
Homo sapiens
57
GMPPB
29925
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
58
CXCR4
7852
Affinity Capture-MS
Homo sapiens
59
MSH2
4436
Co-fractionation
Homo sapiens
60
TIPRL
261726
Co-fractionation
Homo sapiens
61
XPO1
7514
Co-fractionation
Homo sapiens
62
CHST6
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
63
GALNT6
11226
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
64
VAMP3
9341
Affinity Capture-MS
Homo sapiens
65
TMEM246
84302
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
66
ENTPD7
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
67
VBP1
7411
Co-fractionation
Homo sapiens
68
WLS
79971
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
69
EXT1
2131
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
70
DCUN1D1
54165
Co-fractionation
Homo sapiens
71
UBLCP1
Affinity Capture-MS
Homo sapiens
72
RPA3
6119
Proximity Label-MS
Homo sapiens
73
IMPAD1
54928
Affinity Capture-MS
Homo sapiens
74
GALNT7
51809
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
75
DUSP9
Affinity Capture-MS
Homo sapiens
76
TMEM101
Affinity Capture-MS
Homo sapiens
77
EBAG9
9166
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
78
RANBP1
5902
Co-fractionation
Homo sapiens
79
GALNT2
2590
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
80
AARS
16
Co-fractionation
Homo sapiens
81
Eif3e
16341
Affinity Capture-MS
Mus musculus
82
PFDN5
5204
Co-fractionation
Homo sapiens
83
P2RY12
64805
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
84
AIMP1
9255
Co-fractionation
Homo sapiens
85
C1GALT1
56913
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
86
CLPP
8192
Affinity Capture-MS
Homo sapiens
87
PLEKHA4
57664
Affinity Capture-MS
Homo sapiens
88
COPZ1
22818
Affinity Capture-MS
Homo sapiens
89
RUVBL2
10856
Co-fractionation
Homo sapiens
90
NME4
4833
Affinity Capture-MS
Homo sapiens
91
TNFSF14
Affinity Capture-MS
Homo sapiens
92
CUL5
8065
Affinity Capture-MS
Homo sapiens
93
ELAVL1
1994
Affinity Capture-RNA
Homo sapiens
94
MGAT2
4247
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
95
SSBP1
6742
Affinity Capture-MS
Homo sapiens
96
GOLIM4
27333
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
97
GOLPH3L
55204
Cross-Linking-MS (XL-MS)
Homo sapiens
98
P2RX1
5023
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
99
TPST1
8460
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
100
TMEM79
Affinity Capture-MS
Homo sapiens
101
SLC39A5
283375
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
102
STEAP1
26872
Affinity Capture-MS
Homo sapiens
103
TPST2
8459
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
104
SERBP1
26135
Affinity Capture-MS
Homo sapiens
105
SRM
6723
Co-fractionation
Homo sapiens
106
CHSY3
337876
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
107
TNFRSF10D
8793
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which GOLPH3 is involved
No pathways found