Gene description for STAT6
Gene name signal transducer and activator of transcription 6, interleukin-4 induced
Gene symbol STAT6
Other names/aliases D12S1644
IL-4-STAT
STAT6B
STAT6C
Species Homo sapiens
 Database cross references - STAT6
ExoCarta ExoCarta_6778
Vesiclepedia VP_6778
Entrez Gene 6778
HGNC 11368
MIM 601512
UniProt P42226  
 STAT6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for STAT6
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IMP
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    transcription coactivator binding GO:0001223 EXP
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    T-helper 1 cell lineage commitment GO:0002296 IEA
    negative regulation of type 2 immune response GO:0002829 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    defense response GO:0006952 IBA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 IBA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 IMP
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 NAS
    cytokine-mediated signaling pathway GO:0019221 IBA
    cytokine-mediated signaling pathway GO:0019221 IMP
    mammary gland epithelial cell proliferation GO:0033598 IEA
    interleukin-4-mediated signaling pathway GO:0035771 IDA
    interleukin-4-mediated signaling pathway GO:0035771 IMP
    regulation of cell population proliferation GO:0042127 IBA
    response to peptide hormone GO:0043434 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 NAS
    isotype switching to IgE isotypes GO:0048289 IEA
    positive regulation of isotype switching to IgE isotypes GO:0048295 IEA
    growth hormone receptor signaling pathway via JAK-STAT GO:0060397 IBA
    mammary gland morphogenesis GO:0060443 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    regulation of mast cell proliferation GO:0070666 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    cellular response to reactive nitrogen species GO:1902170 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IMP
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    RNA polymerase II transcription regulator complex GO:0090575 IBA
    RNA polymerase II transcription regulator complex GO:0090575 IPI
    RNA polymerase II transcription regulator complex GO:0090575 NAS
 Experiment description of studies that identified STAT6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STAT6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Co-fractionation Homo sapiens
2 ZNF407  
Two-hybrid Homo sapiens
3 KDM3A  
Co-fractionation Homo sapiens
4 NCOA2 10499
Reconstituted Complex Homo sapiens
5 ANKZF1 55139
Two-hybrid Homo sapiens
6 PDE12 201626
Co-fractionation Homo sapiens
7 SFPQ 6421
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
8 SH3BGRL 6451
Co-fractionation Homo sapiens
9 IFNG 3458
Reconstituted Complex Homo sapiens
10 TRIM24  
Two-hybrid Homo sapiens
11 IRF4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 STAT2 6773
Affinity Capture-Western Homo sapiens
13 EEF1A1 1915
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
14 EHD1 10938
Co-fractionation Homo sapiens
15 PSAT1 29968
Co-fractionation Homo sapiens
16 TP53 7157
Two-hybrid Homo sapiens
17 AHCYL1 10768
Co-fractionation Homo sapiens
18 WWP1 11059
Two-hybrid Homo sapiens
19 NUBP2 10101
Co-fractionation Homo sapiens
20 E2F8  
Co-fractionation Homo sapiens
21 SRXN1 140809
Co-fractionation Homo sapiens
22 RB1 5925
Negative Genetic Homo sapiens
23 PPARG 5468
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 APEX1 328
Affinity Capture-RNA Homo sapiens
25 RAD23B 5887
Co-fractionation Homo sapiens
26 NCOA1  
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
27 POLK  
Affinity Capture-MS Homo sapiens
28 FKRP 79147
Affinity Capture-MS Homo sapiens
29 LITAF 9516
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
30 PTPN2 5771
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 CREBBP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
32 ZNF92  
Two-hybrid Homo sapiens
33 TSTA3 7264
Co-fractionation Homo sapiens
34 PGGT1B 5229
Co-fractionation Homo sapiens
35 DDX58 23586
Affinity Capture-RNA Homo sapiens
36 INPPL1 3636
Affinity Capture-MS Homo sapiens
37 SEC24A 10802
Co-fractionation Homo sapiens
38 PARP14 54625
Affinity Capture-Western Homo sapiens
39 IL4R  
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
40 JUN 3725
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 DAZAP2  
Two-hybrid Homo sapiens
42 CEBPE  
Two-hybrid Homo sapiens
43 USP8 9101
Co-fractionation Homo sapiens
44 ZBTB2 57621
Two-hybrid Homo sapiens
45 API5 8539
Co-fractionation Homo sapiens
46 IFNAR1  
Affinity Capture-Western Homo sapiens
47 JAK1 3716
Biochemical Activity Homo sapiens
48 KIF22  
Affinity Capture-MS Homo sapiens
49 NFKB1 4790
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 FCER2 2208
Affinity Capture-RNA Homo sapiens
51 RCN3 57333
Two-hybrid Homo sapiens
52 IGHE  
Affinity Capture-RNA Homo sapiens
53 SSSCA1 10534
Co-fractionation Homo sapiens
54 RLIM 51132
Two-hybrid Homo sapiens
55 USP18  
Affinity Capture-MS Homo sapiens
56 DSTN 11034
Co-fractionation Homo sapiens
57 VKORC1 79001
Two-hybrid Homo sapiens
58 ACTB 60
Co-fractionation Homo sapiens
59 STAT3 6774
Two-hybrid Homo sapiens
60 DHX9 1660
Co-fractionation Homo sapiens
61 ANXA2 302
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
62 NCOA3  
Affinity Capture-Western Homo sapiens
63 CEBPZ  
Two-hybrid Homo sapiens
64 SND1 27044
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
65 PPM1G 5496
Co-fractionation Homo sapiens
66 AACS 65985
Co-fractionation Homo sapiens
67 EP300 2033
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Phenotypic Enhancement Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
68 GART 2618
Co-fractionation Homo sapiens
View the network image/svg+xml



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