Experiment description of studies that identified SPG21 in exosomes

1 Experiment ID 235 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH EV Cytosolic markers ✘ EV Membrane markers ✔ HSP90B1 EV Negative markers ✔ qNano EV Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 217 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101 EV Cytosolic markers ✔ CD81|CD9|CD63 EV Membrane markers ✘ EV Negative markers ✔ NTA EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for SPG21

Protein Interactor ExoCarta ID Identification method PubMed Species 1 C6orf166   Two-hybrid Homo sapiens 2 CD4 920 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens 3 CUTC   Two-hybrid Homo sapiens 4 RABAC1   Two-hybrid Homo sapiens 5 PRPS1 5631 Two-hybrid Homo sapiens 6 TRAF2   Two-hybrid Homo sapiens 7 GGA2   Two-hybrid Homo sapiens 8 MTMR9   Two-hybrid Homo sapiens 9 ZNF263   Two-hybrid Homo sapiens 10 CTPS2 56474 Two-hybrid Homo sapiens 11 RTN4 57142 Two-hybrid Homo sapiens View the network

Pathways in which SPG21 is involved

No pathways found