Gene description for ASH1L
Gene name ash1 (absent, small, or homeotic)-like (Drosophila)
Gene symbol ASH1L
Other names/aliases ASH1
ASH1L1
KMT2H
Species Homo sapiens
 Database cross references - ASH1L
ExoCarta ExoCarta_55870
Entrez Gene 55870
HGNC 19088
MIM 607999
UniProt Q9NR48  
 ASH1L identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ASH1L
Molecular Function
    histone methyltransferase activity (H3-K4 specific) GO:0042800 IEA
    chromatin binding GO:0003682 IEA
    DNA binding GO:0003677 IEA
    zinc ion binding GO:0008270 IEA
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    negative regulation of acute inflammatory response GO:0002674 IEA
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 IEA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    negative regulation of MAPK cascade GO:0043409 IEA
    cell-cell signaling GO:0007267 TAS
    interleukin-6 production GO:0032635 IEA
    DNA packaging GO:0006323 TAS
    histone H3-K4 methylation GO:0051568 IEA
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    bicellular tight junction GO:0005923 IEA
    Golgi apparatus GO:0005794 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IDA
    chromosome GO:0005694 IEA
    nucleus GO:0005634 TAS
 Experiment description of studies that identified ASH1L in exosomes
1
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ASH1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ASH1L is involved
No pathways found





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