Gene description for RPS6KA4
Gene name ribosomal protein S6 kinase, 90kDa, polypeptide 4
Gene symbol RPS6KA4
Other names/aliases MSK2
RSK-B
Species Homo sapiens
 Database cross references - RPS6KA4
ExoCarta ExoCarta_8986
Vesiclepedia VP_8986
Entrez Gene 8986
HGNC 10433
MIM 603606
UniProt O75676  
 RPS6KA4 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for RPS6KA4
Molecular Function
    magnesium ion binding GO:0000287 IEA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 NAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    histone H3S10 kinase activity GO:0035175 IMP
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IMP
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    negative regulation of cytokine production GO:0001818 TAS
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of DNA-templated transcription GO:0006355 IDA
    protein phosphorylation GO:0006468 IDA
    inflammatory response GO:0006954 IEA
    positive regulation of CREB transcription factor activity GO:0032793 TAS
    intracellular signal transduction GO:0035556 IDA
    TORC1 signaling GO:0038202 IBA
    post-translational protein modification GO:0043687 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    interleukin-1-mediated signaling pathway GO:0070498 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    synapse GO:0045202 IEA
 Experiment description of studies that identified RPS6KA4 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS6KA4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MESDC2 23184
Co-fractionation Homo sapiens
2 SCOC 60592
Affinity Capture-MS Homo sapiens
3 DHFR 1719
Affinity Capture-MS Homo sapiens
4 NFKBIB  
Affinity Capture-MS Homo sapiens
5 SENP3 26168
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 MAPK4  
Affinity Capture-MS Homo sapiens
8 CSNK2B 1460
Affinity Capture-MS Homo sapiens
9 PRKY  
Affinity Capture-MS Homo sapiens
10 NEDD4 4734
Reconstituted Complex Homo sapiens
11 LAMP3  
Affinity Capture-MS Homo sapiens
12 DLG3 1741
Co-fractionation Homo sapiens
13 MYLK2 85366
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MAPK1 5594
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
15 MICA 100507436
Affinity Capture-MS Homo sapiens
16 KLHDC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FAM96A  
Affinity Capture-MS Homo sapiens
18 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SULT1C4  
Affinity Capture-MS Homo sapiens
20 MAPK3 5595
Affinity Capture-Western Homo sapiens
21 CLEC4A  
Affinity Capture-MS Homo sapiens
22 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
23 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
24 H1F0 3005
Affinity Capture-MS Homo sapiens
25 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
26 METTL21B  
Affinity Capture-MS Homo sapiens
27 KLHL34  
Affinity Capture-MS Homo sapiens
28 CREB1  
Biochemical Activity Homo sapiens
29 DDX58 23586
Affinity Capture-RNA Homo sapiens
30 DCTN3 11258
Affinity Capture-MS Homo sapiens
31 HIST2H2AA3 8337
Affinity Capture-MS Homo sapiens
32 CDHR4  
Affinity Capture-MS Homo sapiens
33 NCAPH 23397
Affinity Capture-MS Homo sapiens
34 FKBP5 2289
Affinity Capture-MS Homo sapiens
35 HSP90AA4P 3323
Affinity Capture-MS Homo sapiens
36 CDK20  
Affinity Capture-MS Homo sapiens
37 C12orf74  
Affinity Capture-MS Homo sapiens
38 FOS 2353
Biochemical Activity Homo sapiens
39 CD200 4345
Affinity Capture-MS Homo sapiens
40 HECW2  
Affinity Capture-MS Homo sapiens
41 NPAS1  
Affinity Capture-MS Homo sapiens
42 NPTN 27020
Affinity Capture-MS Homo sapiens
43 D2HGDH 728294
Affinity Capture-MS Homo sapiens
44 RFXANK  
Affinity Capture-MS Homo sapiens
45 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
46 TNNC2  
Affinity Capture-MS Homo sapiens
47 FAM174A 345757
Affinity Capture-MS Homo sapiens
48 DDX42 11325
Co-fractionation Homo sapiens
49 B4GALT2  
Affinity Capture-MS Homo sapiens
50 RNF2  
Affinity Capture-MS Homo sapiens
51 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
52 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
53 MAPK14 1432
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
54 CCT8L2  
Affinity Capture-MS Homo sapiens
55 DNAI2  
Affinity Capture-MS Homo sapiens
56 UBXN6 80700
Affinity Capture-MS Homo sapiens
57 BRCA1 672
Affinity Capture-MS Homo sapiens
58 RPS6KA1 6195
Affinity Capture-MS Homo sapiens
59 MAPK11  
Affinity Capture-MS Homo sapiens
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