Gene description for GALNT4
Gene name polypeptide N-acetylgalactosaminyltransferase 4
Gene symbol GALNT4
Other names/aliases GALNAC-T4
GALNACT4
Species Homo sapiens
 Database cross references - GALNT4
ExoCarta ExoCarta_8693
Vesiclepedia VP_8693
Entrez Gene 8693
HGNC 4126
MIM 603565
UniProt Q8N4A0  
 GALNT4 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
 Gene ontology annotations for GALNT4
Molecular Function
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IBA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IDA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 TAS
    protein binding GO:0005515 IPI
    manganese ion binding GO:0030145 IDA
    carbohydrate binding GO:0030246 IEA
Biological Process
    protein O-linked glycosylation GO:0006493 IBA
    O-glycan processing GO:0016266 TAS
    protein O-linked glycosylation via serine GO:0018242 IDA
    protein O-linked glycosylation via threonine GO:0018243 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GALNT4 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for GALNT4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GALNT7 51809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HPN  
Affinity Capture-MS Homo sapiens
3 MUC1 4582
Biochemical Activity Homo sapiens
4 CLEC12B  
Affinity Capture-MS Homo sapiens
5 MPPE1 65258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 APOF 319
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PIANP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 ADPGK 83440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
10 GAA 2548
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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