Gene description for RFT1
Gene name RFT1 homolog (S. cerevisiae)
Gene symbol RFT1
Other names/aliases CDG1N
Species Homo sapiens
 Database cross references - RFT1
ExoCarta ExoCarta_91869
Vesiclepedia VP_91869
Entrez Gene 91869
HGNC 30220
MIM 611908
UniProt Q96AA3  
 RFT1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RFT1
Molecular Function
    protein binding GO:0005515 IPI
    glycolipid floppase activity GO:0034202 TAS
Biological Process
    protein N-linked glycosylation GO:0006487 IMP
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IMP
    glycolipid translocation GO:0034203 IBA
    glycolipid translocation GO:0034203 IGI
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IGI
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified RFT1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RFT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADORA2B  
Affinity Capture-MS Homo sapiens
2 CRELD1 78987
Affinity Capture-MS Homo sapiens
3 GPM6A 2823
Affinity Capture-MS Homo sapiens
4 Tmed2 56334
Affinity Capture-MS Mus musculus
5 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 TSPAN5 10098
Affinity Capture-MS Homo sapiens
8 VIPR2  
Affinity Capture-MS Homo sapiens
9 PSMA3 5684
Two-hybrid Homo sapiens
10 ATP13A3 79572
Affinity Capture-MS Homo sapiens
11 NEDD4 4734
Reconstituted Complex Homo sapiens
12 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 MARCKS 4082
Proximity Label-MS Homo sapiens
15 LTBR 4055
Affinity Capture-MS Homo sapiens
16 NUP155 9631
Proximity Label-MS Homo sapiens
17 RPA2 6118
Proximity Label-MS Homo sapiens
18 TMEM184A  
Affinity Capture-MS Homo sapiens
19 RNF144A  
Two-hybrid Homo sapiens
20 ASGR2  
Affinity Capture-MS Homo sapiens
21 ERGIC3 51614
Two-hybrid Homo sapiens
22 P2RY2 5029
Affinity Capture-MS Homo sapiens
23 HSD3B7 80270
Proximity Label-MS Homo sapiens
24 TSPAN15 23555
Affinity Capture-MS Homo sapiens
25 NIPAL1  
Affinity Capture-MS Homo sapiens
26 FYCO1 79443
Affinity Capture-MS Homo sapiens
27 GPR114  
Affinity Capture-MS Homo sapiens
28 AQP3  
Affinity Capture-MS Homo sapiens
29 MFSD4  
Affinity Capture-MS Homo sapiens
30 ERGIC1 57222
Proximity Label-MS Homo sapiens
31 C5AR1  
Affinity Capture-MS Homo sapiens
32 TMEM171  
Affinity Capture-MS Homo sapiens
33 SLC6A20  
Affinity Capture-MS Homo sapiens
34 RIT1 6016
Negative Genetic Homo sapiens
35 GPR35  
Affinity Capture-MS Homo sapiens
36 GPR52  
Affinity Capture-MS Homo sapiens
37 CD7  
Affinity Capture-MS Homo sapiens
38 SPCS1 28972
Affinity Capture-MS Homo sapiens
39 SKA1  
Affinity Capture-MS Homo sapiens
40 SPPL2B 56928
Affinity Capture-MS Homo sapiens
41 FPR2  
Affinity Capture-MS Homo sapiens
42 TMX2 51075
Two-hybrid Homo sapiens
43 ACKR2  
Affinity Capture-MS Homo sapiens
44 GPR182  
Affinity Capture-MS Homo sapiens
45 LPAR2 9170
Affinity Capture-MS Homo sapiens
46 ASPH 444
Affinity Capture-MS Homo sapiens
47 PTPRN  
Affinity Capture-MS Homo sapiens
48 STOM 2040
Affinity Capture-MS Homo sapiens
49 SCN2B  
Affinity Capture-MS Homo sapiens
50 MUC1 4582
Two-hybrid Homo sapiens
51 RPN1 6184
Proximity Label-MS Homo sapiens
52 KRAS 3845
Negative Genetic Homo sapiens
53 RAB5C 5878
Proximity Label-MS Homo sapiens
54 P2RY12 64805
Affinity Capture-MS Homo sapiens
55 HTR1B  
Affinity Capture-MS Homo sapiens
56 CNR2  
Two-hybrid Homo sapiens
57 GPBAR1  
Affinity Capture-MS Homo sapiens
58 TSPAN12  
Two-hybrid Homo sapiens
59 ADGRE5 976
Affinity Capture-MS Homo sapiens
60 SEC61B 10952
Proximity Label-MS Homo sapiens
61 CREB3L1  
Two-hybrid Homo sapiens
62 ZACN  
Affinity Capture-MS Homo sapiens
63 CCDC47 57003
Affinity Capture-MS Homo sapiens
64 ATP2A1 487
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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