Gene description for Hnrnpa2b1
Gene name heterogeneous nuclear ribonucleoprotein A2/B1
Gene symbol Hnrnpa2b1
Other names/aliases 9130414A06Rik
Hnrpa2
Hnrpa2b1
hnrnp-A
Species Mus musculus
 Database cross references - Hnrnpa2b1
ExoCarta ExoCarta_53379
Vesiclepedia VP_53379
Entrez Gene 53379
UniProt O88569  
 Hnrnpa2b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hnrnpa2b1
Molecular Function
    regulatory region RNA binding GO:0001069 ISO
    RNA binding GO:0003723 IPI
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    mRNA 3'-UTR binding GO:0003730 IBA
    mRNA 3'-UTR binding GO:0003730 ISO
    mRNA 3'-UTR binding GO:0003730 ISS
    miRNA binding GO:0035198 ISO
    miRNA binding GO:0035198 ISS
    identical protein binding GO:0042802 ISO
    single-stranded telomeric DNA binding GO:0043047 IBA
    single-stranded telomeric DNA binding GO:0043047 ISO
    single-stranded telomeric DNA binding GO:0043047 ISS
    DNA polymerase binding GO:0070182 ISO
    pre-mRNA intronic binding GO:0097157 IDA
    G-rich strand telomeric DNA binding GO:0098505 ISO
    molecular condensate scaffold activity GO:0140693 ISO
    N6-methyladenosine-containing RNA reader activity GO:1990247 ISO
    N6-methyladenosine-containing RNA reader activity GO:1990247 ISS
    mRNA CDS binding GO:1990715 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    mRNA processing GO:0006397 ISO
    mRNA processing GO:0006397 ISS
    mRNA export from nucleus GO:0006406 ISO
    mRNA export from nucleus GO:0006406 ISS
    telomere capping GO:0016233 ISO
    primary miRNA processing GO:0031053 ISO
    primary miRNA processing GO:0031053 ISS
    G-quadruplex DNA unwinding GO:0044806 ISO
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IMP
    RNA transport GO:0050658 ISO
    RNA transport GO:0050658 ISS
    mRNA transport GO:0051028 IBA
    positive regulation of telomere maintenance via telomere lengthening GO:1904358 ISO
    miRNA transport GO:1990428 ISO
    miRNA transport GO:1990428 ISS
Subcellular Localization
    chromosome, telomeric region GO:0000781 ISO
    chromatin GO:0000785 ISO
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 ISO
    spliceosomal complex GO:0005681 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    postsynaptic density GO:0014069 ISO
    Cajal body GO:0015030 ISO
    nuclear matrix GO:0016363 ISO
    neuronal cell body GO:0043025 ISO
    perikaryon GO:0043204 ISO
    extracellular exosome GO:0070062 ISO
    extracellular exosome GO:0070062 ISS
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 ISO
    neuronal ribonucleoprotein granule GO:0071598 ISO
    glutamatergic synapse GO:0098978 ISO
    postsynaptic cytosol GO:0099524 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Hnrnpa2b1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hnrnpa2b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl39 67248
Co-fractionation Mus musculus
4 Ncbp1 433702
Co-fractionation Mus musculus
5 SNCA 6622
Affinity Capture-MS Homo sapiens
6 Snrnp200 320632
Co-fractionation Mus musculus
7 MKRN1 23608
Affinity Capture-MS Homo sapiens
8 Snrpd1 20641
Co-fractionation Mus musculus
9 Stau1  
Co-fractionation Mus musculus
10 Prpf8 192159
Co-fractionation Mus musculus
11 Rps4x 20102
Co-fractionation Mus musculus
12 Plrg1  
Co-fractionation Mus musculus
13 Sf3b1 81898
Co-fractionation Mus musculus
14 Snrpd2 107686
Co-fractionation Mus musculus
15 Sf1 22668
Co-fractionation Mus musculus
16 Rps28 54127
Co-fractionation Mus musculus
17 Kctd13  
Affinity Capture-MS Mus musculus
18 Tfe3  
Affinity Capture-MS Mus musculus
19 Rbm3  
Co-fractionation Mus musculus
20 Frg1  
Co-fractionation Mus musculus
21 Set 56086
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
22 Sf3a1 67465
Co-fractionation Mus musculus
23 Snrpd3 67332
Co-fractionation Mus musculus
24 Sf3b3 101943
Co-fractionation Mus musculus
25 Rbm8a  
Co-fractionation Mus musculus
26 Tmem173  
Proximity Label-MS Mus musculus
27 Ybx1 22608
Co-fractionation Mus musculus
28 Ppp2ca 19052
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
29 Ctnnbl1  
Co-fractionation Mus musculus
30 Rps9 76846
Co-fractionation Mus musculus
31 Hnrnpc 15381
Co-fractionation Mus musculus
32 Srrm2  
Co-fractionation Mus musculus
33 Dmrt2  
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
34 Sra1  
Co-fractionation Mus musculus
35 Syncrip 56403
Co-fractionation Mus musculus
36 Snw1  
Co-fractionation Mus musculus
37 Rps3a1 20091
Co-fractionation Mus musculus
38 Ppwd1  
Co-fractionation Mus musculus
39 Ddx1 104721
Co-fractionation Mus musculus
40 Rpl26 19941
Co-fractionation Mus musculus
41 Eprs 107508
Co-fractionation Mus musculus
42 Rplp0 11837
Co-fractionation Mus musculus
43 Skiv2l2  
Co-fractionation Mus musculus
44 Ppil3  
Co-fractionation Mus musculus
45 Eed  
Affinity Capture-MS Mus musculus
46 Cwc27  
Co-fractionation Mus musculus
47 U2af2 22185
Co-fractionation Mus musculus
48 Atg16l1  
Affinity Capture-MS Mus musculus
49 Eif4a3 192170
Co-fractionation Mus musculus
50 Fancd2  
Affinity Capture-MS Mus musculus
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