Gene description for Eef1b2
Gene name eukaryotic translation elongation factor 1 beta 2
Gene symbol Eef1b2
Other names/aliases 2810017J07Rik
Eef1b
Species Mus musculus
 Database cross references - Eef1b2
ExoCarta ExoCarta_55949
Vesiclepedia VP_55949
Entrez Gene 55949
UniProt O70251  
 Eef1b2 identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eef1b2
Molecular Function
    translation elongation factor activity GO:0003746 ISS
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    protein binding GO:0005515 IPI
Biological Process
    translational elongation GO:0006414 IBA
    translational elongation GO:0006414 IEA
    response to ethanol GO:0045471 IEA
    response to ethanol GO:0045471 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 IBA
    eukaryotic translation elongation factor 1 complex GO:0005853 ISS
 Experiment description of studies that identified Eef1b2 in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eef1b2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Rpl35 66489
Co-fractionation Mus musculus
5 Rps18 20084
Co-fractionation Mus musculus
6 Rps19 20085
Co-fractionation Mus musculus
7 Rps28 54127
Co-fractionation Mus musculus
8 Rps26 27370
Co-fractionation Mus musculus
9 Rpl13a 22121
Co-fractionation Mus musculus
10 Rpl15 66480
Co-fractionation Mus musculus
11 Rplp2 67186
Co-fractionation Mus musculus
12 Rpl38  
Co-fractionation Mus musculus
13 Rps4x 20102
Co-fractionation Mus musculus
14 Eif5a2  
Co-fractionation Mus musculus
15 Rpl14 67115
Co-fractionation Mus musculus
16 Rps2 16898
Co-fractionation Mus musculus
17 Rps8 20116
Co-fractionation Mus musculus
18 Rps23 66475
Co-fractionation Mus musculus
19 Rpl7a 27176
Co-fractionation Mus musculus
20 Rpl31 114641
Co-fractionation Mus musculus
21 Vars 22321
Co-fractionation Mus musculus
22 Rpl32 19951
Co-fractionation Mus musculus
23 Rpl6 19988
Co-fractionation Mus musculus
24 Kctd13  
Affinity Capture-MS Mus musculus
25 Thrb  
Affinity Capture-MS Mus musculus
26 Rps3a1 20091
Co-fractionation Mus musculus
27 Tfe3  
Affinity Capture-MS Mus musculus
28 Rpl24 68193
Co-fractionation Mus musculus
29 Tpt1 22070
Co-fractionation Mus musculus
30 Rps11 27207
Co-fractionation Mus musculus
31 Rpl18a 76808
Co-fractionation Mus musculus
32 Rps24 20088
Co-fractionation Mus musculus
33 Ywhaz 22631
Affinity Capture-MS Mus musculus
34 Rps7 20115
Co-fractionation Mus musculus
35 Rpl22 19934
Co-fractionation Mus musculus
36 Tmem173  
Proximity Label-MS Mus musculus
37 Rpl19 19921
Co-fractionation Mus musculus
38 Rps20 67427
Co-fractionation Mus musculus
39 Rps13 68052
Co-fractionation Mus musculus
40 MAPT  
Reconstituted Complex Homo sapiens
41 Rpl18 19899
Co-fractionation Mus musculus
42 Rps9 76846
Co-fractionation Mus musculus
43 Srrm2  
Co-fractionation Mus musculus
44 Rpl12 269261
Co-fractionation Mus musculus
45 Rps15a 267019
Co-fractionation Mus musculus
46 Gnb2l1 14694
Co-fractionation Mus musculus
47 Rps5 20103
Co-fractionation Mus musculus
48 Rpl7 19989
Co-fractionation Mus musculus
49 Rpl26 19941
Co-fractionation Mus musculus
50 Rpl13 270106
Co-fractionation Mus musculus
51 Rplp0 11837
Co-fractionation Mus musculus
52 Rps21 66481
Co-fractionation Mus musculus
53 Rps14 20044
Co-fractionation Mus musculus
54 Rps17 20068
Co-fractionation Mus musculus
55 Rpl30 19946
Co-fractionation Mus musculus
56 Eed  
Affinity Capture-MS Mus musculus
57 Rpsa 16785
Co-fractionation Mus musculus
58 Ywhae 22627
Affinity Capture-MS Mus musculus
59 Rpl3 27367
Co-fractionation Mus musculus
60 Rpl4 67891
Co-fractionation Mus musculus
61 Fancd2  
Affinity Capture-MS Mus musculus
62 Rpl8 26961
Co-fractionation Mus musculus
63 Tcf3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Eef1b2 is involved
PathwayEvidenceSource
Eukaryotic Translation Elongation IEA Reactome
Metabolism of proteins IEA Reactome
Translation IEA Reactome





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