Gene description for Rpl6
Gene name ribosomal protein L6
Gene symbol Rpl6
Other names/aliases Taxreb107
Species Mus musculus
 Database cross references - Rpl6
ExoCarta ExoCarta_19988
Vesiclepedia VP_19988
Entrez Gene 19988
UniProt P47911  
 Rpl6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl6
Molecular Function
    tRNA binding GO:0000049 ISO
    RNA binding GO:0003723 IBA
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    5.8S rRNA binding GO:1990932 ISO
Biological Process
    cytoplasmic translation GO:0002181 IBA
    cytoplasmic translation GO:0002181 ISO
    cytoplasmic translation GO:0002181 ISS
    cytoplasmic translation GO:0002181 NAS
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    rough endoplasmic reticulum GO:0005791 IEA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 EXP
    postsynaptic density GO:0014069 IDA
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic large ribosomal subunit GO:0022625 ISS
    cytosolic ribosome GO:0022626 ISO
    A band GO:0031672 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Hsp90ab1 15516
Co-fractionation Mus musculus
4 Ina  
Co-fractionation Mus musculus
5 Rpl38  
Co-fractionation Mus musculus
6 Raver1  
Co-fractionation Mus musculus
7 Rpl27 19942
Co-fractionation Mus musculus
8 Fmr1  
Co-fractionation Mus musculus
9 Rps6 20104
Co-fractionation Mus musculus
10 Ncl 17975
Co-fractionation Mus musculus
11 Rcsd1  
Co-fractionation Mus musculus
12 Rpl35a 57808
Co-fractionation Mus musculus
13 Hsp90aa1 15519
Co-fractionation Mus musculus
14 Tmem173  
Proximity Label-MS Mus musculus
15 Rpl7 19989
Co-fractionation Mus musculus
16 Eef1a1 13627
Co-fractionation Mus musculus
17 Plod3 26433
Co-fractionation Mus musculus
18 Ddx3x 13205
Co-fractionation Mus musculus
19 Dync1i1  
Co-fractionation Mus musculus
20 AI314180 230249
Co-fractionation Mus musculus
21 Rpl36a 19982
Co-fractionation Mus musculus
22 Rpl26 19941
Co-fractionation Mus musculus
23 Rpsa 16785
Co-fractionation Mus musculus
24 Rpl3 27367
Co-fractionation Mus musculus
25 Abce1 24015
Co-fractionation Mus musculus
26 Rpl11 67025
Co-fractionation Mus musculus
27 Eed  
Affinity Capture-MS Mus musculus
28 Rpl31 114641
Co-fractionation Mus musculus
29 Rps10 67097
Co-fractionation Mus musculus
30 Sarnp 66118
Co-fractionation Mus musculus
31 Stau1  
Co-fractionation Mus musculus
32 Rplp2 67186
Co-fractionation Mus musculus
33 Rps4x 20102
Co-fractionation Mus musculus
34 Rps23 66475
Co-fractionation Mus musculus
35 Rpl14 67115
Co-fractionation Mus musculus
36 Scyl2  
Co-fractionation Mus musculus
37 Mapt  
Co-fractionation Mus musculus
38 Eif6 16418
Co-fractionation Mus musculus
39 Tmem165  
Co-fractionation Mus musculus
40 Tfe3  
Affinity Capture-MS Mus musculus
41 Rpl24 68193
Co-fractionation Mus musculus
42 Rps7 20115
Co-fractionation Mus musculus
43 Rpl17 319195
Co-fractionation Mus musculus
44 Rpl5 100503670
Co-fractionation Mus musculus
45 Pabpc1 18458
Co-fractionation Mus musculus
46 Gtf2f1  
Co-fractionation Mus musculus
47 Rps5 20103
Co-fractionation Mus musculus
48 Rps15a 267019
Co-fractionation Mus musculus
49 Rock2  
Co-fractionation Mus musculus
50 Ppwd1  
Co-fractionation Mus musculus
51 Poldip3  
Co-fractionation Mus musculus
52 Fancd2  
Affinity Capture-MS Mus musculus
53 Dhx9 13211
Co-fractionation Mus musculus
54 Rpl30 19946
Co-fractionation Mus musculus
55 Rpl39 67248
Co-fractionation Mus musculus
56 Rps16 20055
Co-fractionation Mus musculus
57 Zfp330  
Co-fractionation Mus musculus
58 Rps17 20068
Co-fractionation Mus musculus
59 Rps28 54127
Co-fractionation Mus musculus
60 Dhx29  
Co-fractionation Mus musculus
61 Rps18 20084
Co-fractionation Mus musculus
62 Rpl13a 22121
Co-fractionation Mus musculus
63 Psmc2 19181
Co-fractionation Mus musculus
64 Rps2 16898
Co-fractionation Mus musculus
65 Rps8 20116
Co-fractionation Mus musculus
66 Rpl7a 27176
Co-fractionation Mus musculus
67 Rpl32 19951
Co-fractionation Mus musculus
68 Kcna3  
Affinity Capture-MS Mus musculus
69 Rps19 20085
Co-fractionation Mus musculus
70 Tpt1 22070
Co-fractionation Mus musculus
71 Rps11 27207
Co-fractionation Mus musculus
72 Rpl18a 76808
Co-fractionation Mus musculus
73 Lrrc59  
Co-fractionation Mus musculus
74 Rpl22 19934
Co-fractionation Mus musculus
75 Rpl19 19921
Co-fractionation Mus musculus
76 Rps24 20088
Co-fractionation Mus musculus
77 Rpl21 19933
Co-fractionation Mus musculus
78 Rplp0 11837
Co-fractionation Mus musculus
79 Rps3a1 20091
Co-fractionation Mus musculus
80 Rps21 66481
Co-fractionation Mus musculus
81 Rps14 20044
Co-fractionation Mus musculus
82 Rpl35 66489
Co-fractionation Mus musculus
83 Dhfr 13361
Co-fractionation Mus musculus
84 Tia1  
Affinity Capture-MS Mus musculus
85 Rpl29 19944
Co-fractionation Mus musculus
86 Rps20 67427
Co-fractionation Mus musculus
87 Usp46  
Co-fractionation Mus musculus
88 Sf3a3 75062
Co-fractionation Mus musculus
89 Ebna1bp2  
Co-fractionation Mus musculus
90 Rps25 75617
Co-fractionation Mus musculus
91 Top1 21969
Co-fractionation Mus musculus
92 Atxn1  
Proximity Label-MS Mus musculus
93 Kctd13  
Affinity Capture-MS Mus musculus
94 Eef1g 67160
Co-fractionation Mus musculus
95 Prpf3 70767
Co-fractionation Mus musculus
96 Fzr1  
Affinity Capture-MS Mus musculus
97 Rpl12 269261
Co-fractionation Mus musculus
98 Rpl18 19899
Co-fractionation Mus musculus
99 Gnb2l1 14694
Co-fractionation Mus musculus
100 Rps9 76846
Co-fractionation Mus musculus
101 Rps13 68052
Co-fractionation Mus musculus
102 Tcf25  
Co-fractionation Mus musculus
103 Rpl23 65019
Co-fractionation Mus musculus
104 Rpl23a 268449
Co-fractionation Mus musculus
105 Rpl15 66480
Co-fractionation Mus musculus
106 Eef1b2 55949
Co-fractionation Mus musculus
107 Rpl4 67891
Co-fractionation Mus musculus
108 Otx2  
Affinity Capture-MS Mus musculus
109 Rpl13 270106
Co-fractionation Mus musculus
110 Rpl8 26961
Co-fractionation Mus musculus
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