Gene description for Top1
Gene name topoisomerase (DNA) I
Gene symbol Top1
Other names/aliases AI467334
D130064I21Rik
Top-1
Species Mus musculus
 Database cross references - Top1
ExoCarta ExoCarta_21969
Vesiclepedia VP_21969
Entrez Gene 21969
UniProt Q04750  
 Top1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Top1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISO
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISS
    DNA binding GO:0003677 ISO
    chromatin binding GO:0003682 IEA
    chromatin binding GO:0003682 ISO
    double-stranded DNA binding GO:0003690 ISO
    single-stranded DNA binding GO:0003697 IEA
    single-stranded DNA binding GO:0003697 ISO
    DNA topoisomerase type I (single strand cut, ATP-independent) activity GO:0003917 IBA
    DNA topoisomerase type I (single strand cut, ATP-independent) activity GO:0003917 IDA
    DNA topoisomerase type I (single strand cut, ATP-independent) activity GO:0003917 ISO
    protein serine/threonine kinase activity GO:0004674 IEA
    protein serine/threonine kinase activity GO:0004674 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    DNA binding, bending GO:0008301 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    chromatin DNA binding GO:0031490 ISO
    protein-containing complex binding GO:0044877 ISO
    supercoiled DNA binding GO:0097100 IEA
    supercoiled DNA binding GO:0097100 ISO
Biological Process
    DNA replication GO:0006260 IBA
    DNA replication GO:0006260 IMP
    DNA topological change GO:0006265 IBA
    DNA topological change GO:0006265 IDA
    DNA topological change GO:0006265 ISO
    chromatin remodeling GO:0006338 ISO
    chromatin remodeling GO:0006338 ISS
    chromosome segregation GO:0007059 IBA
    rRNA transcription GO:0009303 ISO
    response to xenobiotic stimulus GO:0009410 IEA
    peptidyl-serine phosphorylation GO:0018105 ISO
    circadian regulation of gene expression GO:0032922 ISO
    circadian regulation of gene expression GO:0032922 ISS
    embryonic cleavage GO:0040016 IMP
Subcellular Localization
    nuclear chromosome GO:0000228 IEA
    nuclear chromosome GO:0000228 ISO
    P-body GO:0000932 IEA
    P-body GO:0000932 ISO
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    dense fibrillar component GO:0001651 ISO
    male germ cell nucleus GO:0001673 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    protein-DNA complex GO:0032993 IEA
    protein-DNA complex GO:0032993 ISO
    perikaryon GO:0043204 IDA
 Experiment description of studies that identified Top1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Top1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vezf1  
Affinity Capture-MS Mus musculus
2 Sf3a3 75062
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Elf5  
Affinity Capture-MS Mus musculus
5 Ebna1bp2  
Co-fractionation Mus musculus
6 Lars 107045
Co-fractionation Mus musculus
7 Rfc1  
Co-fractionation Mus musculus
8 Rpl13a 22121
Co-fractionation Mus musculus
9 St18  
Co-fractionation Mus musculus
10 Dhx29  
Co-fractionation Mus musculus
11 Rps4x 20102
Co-fractionation Mus musculus
12 Rps2 16898
Co-fractionation Mus musculus
13 Prim1  
Co-fractionation Mus musculus
14 Rpl7a 27176
Co-fractionation Mus musculus
15 Scyl2  
Co-fractionation Mus musculus
16 Xrcc5  
Co-fractionation Mus musculus
17 Irf8  
Co-fractionation Mus musculus
18 Foxp3  
Affinity Capture-MS Mus musculus
19 Cebpa  
Affinity Capture-MS Mus musculus
20 Cul4b  
Affinity Capture-MS Mus musculus
21 Rps6 20104
Co-fractionation Mus musculus
22 Naa35  
Co-fractionation Mus musculus
23 Rpl35a 57808
Co-fractionation Mus musculus
24 Lrrc59  
Co-fractionation Mus musculus
25 Rpl22 19934
Co-fractionation Mus musculus
26 Zbtb7b  
Affinity Capture-MS Mus musculus
27 Gtf2f1  
Co-fractionation Mus musculus
28 JADE1  
Affinity Capture-MS Homo sapiens
29 Oas3  
Co-fractionation Mus musculus
30 AI314180 230249
Co-fractionation Mus musculus
31 Atp9a  
Co-fractionation Mus musculus
32 Casp6 12368
Co-fractionation Mus musculus
33 Rps3a1 20091
Co-fractionation Mus musculus
34 Ppwd1  
Co-fractionation Mus musculus
35 Top2a 21973
Co-fractionation Mus musculus
36 Rps14 20044
Co-fractionation Mus musculus
37 Eed  
Affinity Capture-MS Mus musculus
38 Rpl15 66480
Co-fractionation Mus musculus
39 Parp1 11545
Co-fractionation Mus musculus
40 Atg16l1  
Affinity Capture-MS Mus musculus
41 Vprbp  
Affinity Capture-MS Mus musculus
42 Rpl6 19988
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here