Gene description for Rps2
Gene name ribosomal protein S2
Gene symbol Rps2
Other names/aliases Llrep3
S4
Species Mus musculus
 Database cross references - Rps2
ExoCarta ExoCarta_16898
Vesiclepedia VP_16898
Entrez Gene 16898
UniProt P25444  
 Rps2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps2
Molecular Function
    RNA binding GO:0003723 IEA
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    fibroblast growth factor binding GO:0017134 ISO
    enzyme binding GO:0019899 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    translation GO:0006412 IBA
    positive regulation of ubiquitin-protein transferase activity GO:0051443 ISO
    cellular response to interleukin-4 GO:0071353 IDA
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    postsynapse GO:0098794 NAS
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rps2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Lars 107045
Co-fractionation Mus musculus
3 Raver1  
Co-fractionation Mus musculus
4 Rpl27 19942
Co-fractionation Mus musculus
5 Rpl6 19988
Co-fractionation Mus musculus
6 Snd1 56463
Co-fractionation Mus musculus
7 Rps24 20088
Co-fractionation Mus musculus
8 Ngly1  
Two-hybrid Mus musculus
Co-fractionation Mus musculus
9 Tmem173  
Proximity Label-MS Mus musculus
10 Rpl7 19989
Co-fractionation Mus musculus
11 Eef1a1 13627
Co-fractionation Mus musculus
12 Rps9 76846
Co-fractionation Mus musculus
13 AI314180 230249
Co-fractionation Mus musculus
14 Rpl26 19941
Co-fractionation Mus musculus
15 Rpsa 16785
Co-fractionation Mus musculus
16 Rpl3 27367
Co-fractionation Mus musculus
17 Abce1 24015
Co-fractionation Mus musculus
18 Tcf25  
Co-fractionation Mus musculus
19 Eed  
Affinity Capture-MS Mus musculus
20 Rpl31 114641
Co-fractionation Mus musculus
21 Rps10 67097
Co-fractionation Mus musculus
22 Rplp2 67186
Co-fractionation Mus musculus
23 Rps4x 20102
Co-fractionation Mus musculus
24 Rps23 66475
Co-fractionation Mus musculus
25 Scyl2  
Co-fractionation Mus musculus
26 Vars 22321
Co-fractionation Mus musculus
27 Tprkb  
Co-fractionation Mus musculus
28 Rpl24 68193
Co-fractionation Mus musculus
29 Rps7 20115
Co-fractionation Mus musculus
30 Rpl17 319195
Co-fractionation Mus musculus
31 Rpl29 19944
Co-fractionation Mus musculus
32 Uggt1 320011
Co-fractionation Mus musculus
33 Rps5 20103
Co-fractionation Mus musculus
34 Rps15a 267019
Co-fractionation Mus musculus
35 Ppwd1  
Co-fractionation Mus musculus
36 Fancd2  
Affinity Capture-MS Mus musculus
37 Rplp0 11837
Co-fractionation Mus musculus
38 Rpl30 19946
Co-fractionation Mus musculus
39 Rpl39 67248
Co-fractionation Mus musculus
40 Rps16 20055
Co-fractionation Mus musculus
41 Rps17 20068
Co-fractionation Mus musculus
42 Sf3a3 75062
Co-fractionation Mus musculus
43 Rps18 20084
Co-fractionation Mus musculus
44 Rpl13a 22121
Co-fractionation Mus musculus
45 Rpl14 67115
Co-fractionation Mus musculus
46 Rps8 20116
Co-fractionation Mus musculus
47 Rpl7a 27176
Co-fractionation Mus musculus
48 Pde12  
Co-fractionation Mus musculus
49 Rpl32 19951
Co-fractionation Mus musculus
50 Rps28 54127
Co-fractionation Mus musculus
51 Rps19 20085
Co-fractionation Mus musculus
52 Trim59  
Two-hybrid Mus musculus
53 Tpt1 22070
Co-fractionation Mus musculus
54 Rps11 27207
Co-fractionation Mus musculus
55 Rpl18a 76808
Co-fractionation Mus musculus
56 Lrrc59  
Co-fractionation Mus musculus
57 Rpl22 19934
Co-fractionation Mus musculus
58 Rps6 20104
Co-fractionation Mus musculus
59 Rpl38  
Co-fractionation Mus musculus
60 Rps3a1 20091
Co-fractionation Mus musculus
61 Rps21 66481
Co-fractionation Mus musculus
62 Rps14 20044
Co-fractionation Mus musculus
63 Rpl35 66489
Co-fractionation Mus musculus
64 Rpl5 100503670
Co-fractionation Mus musculus
65 Rps20 67427
Co-fractionation Mus musculus
66 Rps12 20042
Co-fractionation Mus musculus
67 Rps27a 78294
Co-fractionation Mus musculus
68 Ubr4 69116
Co-fractionation Mus musculus
69 Rps25 75617
Co-fractionation Mus musculus
70 Rpl10a 19896
Co-fractionation Mus musculus
71 Top1 21969
Co-fractionation Mus musculus
72 Rbm39 170791
Co-fractionation Mus musculus
73 Kctd13  
Affinity Capture-MS Mus musculus
74 Prpf3 70767
Co-fractionation Mus musculus
75 Fzr1  
Affinity Capture-MS Mus musculus
76 Eef2 13629
Co-fractionation Mus musculus
77 Rpl12 269261
Co-fractionation Mus musculus
78 Rpl18 19899
Co-fractionation Mus musculus
79 Gnb2l1 14694
Co-fractionation Mus musculus
80 Rps15 20054
Co-fractionation Mus musculus
81 Rps13 68052
Co-fractionation Mus musculus
82 Hba 15121
Co-fractionation Mus musculus
83 Rpl23 65019
Co-fractionation Mus musculus
84 Rpl23a 268449
Co-fractionation Mus musculus
85 Rpl15 66480
Co-fractionation Mus musculus
86 Eef1b2 55949
Co-fractionation Mus musculus
87 Rpl4 67891
Co-fractionation Mus musculus
88 Rpl13 270106
Co-fractionation Mus musculus
89 Rpl8 26961
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here