Gene description for Actn4
Gene name actinin alpha 4
Gene symbol Actn4
Other names/aliases C77391
Species Mus musculus
 Database cross references - Actn4
ExoCarta ExoCarta_60595
Vesiclepedia VP_60595
Entrez Gene 60595
UniProt P57780  
 Actn4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Fibroblasts 23260141    
Mast cells 17486113    
Mov neuroglial cells 15210972    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Actn4
Molecular Function
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 ISO
    nucleoside binding GO:0001882 ISO
    transcription coactivator activity GO:0003713 ISO
    structural constituent of cytoskeleton GO:0005200 IMP
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    nuclear receptor binding GO:0016922 ISO
    nuclear receptor coactivator activity GO:0030374 ISO
    nuclear receptor coactivator activity GO:0030374 ISS
    chromatin DNA binding GO:0031490 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 ISO
    nuclear retinoic acid receptor binding GO:0042974 ISO
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
    actin filament binding GO:0051015 ISO
Biological Process
    gene expression GO:0010467 IMP
    protein transport GO:0015031 IEA
    actin cytoskeleton organization GO:0030036 IBA
    vesicle transport along actin filament GO:0030050 ISO
    positive regulation of cell migration GO:0030335 ISO
    glomerulus development GO:0032835 IDA
    tumor necrosis factor-mediated signaling pathway GO:0033209 ISO
    peroxisome proliferator activated receptor signaling pathway GO:0035357 ISO
    peroxisome proliferator activated receptor signaling pathway GO:0035357 ISS
    regulation of apoptotic process GO:0042981 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    retinoic acid receptor signaling pathway GO:0048384 ISO
    retinoic acid receptor signaling pathway GO:0048384 ISS
    positive regulation of pinocytosis GO:0048549 IMP
    actin filament bundle assembly GO:0051017 IDA
    muscle cell development GO:0055001 IBA
    bicellular tight junction assembly GO:0070830 IMP
    podocyte cell migration GO:0090521 IMP
    negative regulation of substrate adhesion-dependent cell spreading GO:1900025 ISO
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISO
    protein localization to bicellular tight junction GO:1902396 IMP
Subcellular Localization
    stress fiber GO:0001725 IDA
    stress fiber GO:0001725 IDA
    stress fiber GO:0001725 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IBA
    cell-cell junction GO:0005911 IDA
    focal adhesion GO:0005925 IDA
    actin cytoskeleton GO:0015629 ISO
    actin cytoskeleton GO:0015629 ISO
    Z disc GO:0030018 IBA
    Z disc GO:0030018 IDA
    cell junction GO:0030054 IBA
    cortical cytoskeleton GO:0030863 IDA
    cortical actin cytoskeleton GO:0030864 IBA
    cortical actin cytoskeleton GO:0030864 IDA
    pseudopodium GO:0031143 IDA
    protein-containing complex GO:0032991 ISO
    cell projection GO:0042995 IBA
    neuron projection GO:0043005 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    postsynaptic actin cytoskeleton GO:0098871 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Actn4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
6
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Actn4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Raver1  
Two-hybrid Mus musculus
4 Rpl13a 22121
Co-fractionation Mus musculus
5 Rps4x 20102
Co-fractionation Mus musculus
6 Tjp1  
Affinity Capture-Western Mus musculus
7 Trove2 20822
Co-fractionation Mus musculus
8 Ybx1 22608
Co-fractionation Mus musculus
9 Myh6  
Cross-Linking-MS (XL-MS) Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Tia1  
Affinity Capture-MS Mus musculus
12 Ncl 17975
Co-fractionation Mus musculus
13 Iqcb1  
Affinity Capture-MS Mus musculus
14 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
15 Agap2  
Affinity Capture-MS Mus musculus
16 Sra1  
Co-fractionation Mus musculus
17 Syncrip 56403
Co-fractionation Mus musculus
18 Rps3a1 20091
Co-fractionation Mus musculus
19 HACE1 57531
Affinity Capture-MS Homo sapiens
20 Atp6v1a 11964
Affinity Capture-Western Mus musculus
21 Rpl26 19941
Co-fractionation Mus musculus
22 ADORA2A  
Two-hybrid Homo sapiens
23 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
24 Eed  
Affinity Capture-MS Mus musculus
25 Trim3  
Co-fractionation Mus musculus
26 Atg16l1  
Affinity Capture-MS Mus musculus
27 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here