Gene description for Rplp0
Gene name ribosomal protein, large, P0
Gene symbol Rplp0
Other names/aliases 36B4
Arbp
L10E
Species Mus musculus
 Database cross references - Rplp0
ExoCarta ExoCarta_11837
Vesiclepedia VP_11837
Entrez Gene 11837
UniProt P14869  
 Rplp0 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Neural stem cells 25242146    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rplp0
Molecular Function
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 ISO
    peptide binding GO:0042277 IEA
    peptide binding GO:0042277 ISO
    large ribosomal subunit rRNA binding GO:0070180 IBA
Biological Process
    cytoplasmic translation GO:0002181 IBA
    cytoplasmic translation GO:0002181 NAS
    lactation GO:0007595 IEA
    response to selenium ion GO:0010269 IEA
    ribosome biogenesis GO:0042254 IEA
    cellular response to cAMP GO:0071320 IEA
    cellular response to interleukin-4 GO:0071353 IDA
    cellular response to Thyroid stimulating hormone GO:1904401 IEA
    cellular response to phorbol 13-acetate 12-myristate GO:1904628 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    dendrite GO:0030425 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    synapse GO:0045202 ISO
    postsynapse GO:0098794 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Rplp0 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rplp0
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Atxn2 20239
Co-fractionation Mus musculus
4 Rpl27 19942
Co-fractionation Mus musculus
5 Ybx1 22608
Co-fractionation Mus musculus
6 Fmr1  
Co-fractionation Mus musculus
7 Rpl6 19988
Co-fractionation Mus musculus
8 Rps24 20088
Co-fractionation Mus musculus
9 Ncl 17975
Co-fractionation Mus musculus
10 Rpl7 19989
Co-fractionation Mus musculus
11 Eef1a1 13627
Co-fractionation Mus musculus
12 Rps9 76846
Co-fractionation Mus musculus
13 Rpl36a 19982
Co-fractionation Mus musculus
14 Rpl26 19941
Co-fractionation Mus musculus
15 Ctsh  
Co-fractionation Mus musculus
16 Rpsa 16785
Co-fractionation Mus musculus
17 Rpl3 27367
Co-fractionation Mus musculus
18 Rpl11 67025
Co-fractionation Mus musculus
19 Eed  
Affinity Capture-MS Mus musculus
20 Rpl31 114641
Co-fractionation Mus musculus
21 Rps10 67097
Co-fractionation Mus musculus
22 Sarnp 66118
Co-fractionation Mus musculus
23 Stau1  
Co-fractionation Mus musculus
24 Rplp2 67186
Co-fractionation Mus musculus
25 Rps4x 20102
Co-fractionation Mus musculus
26 Trove2 20822
Co-fractionation Mus musculus
27 Rps23 66475
Co-fractionation Mus musculus
28 Mapt  
Affinity Capture-MS Mus musculus
29 Tfe3  
Affinity Capture-MS Mus musculus
30 Rpl24 68193
Co-fractionation Mus musculus
31 Lsm14a  
Co-fractionation Mus musculus
32 Pabpn1 54196
Co-fractionation Mus musculus
33 Rps7 20115
Co-fractionation Mus musculus
34 Rpl17 319195
Co-fractionation Mus musculus
35 Ppia 268373
Co-fractionation Mus musculus
36 Rps5 20103
Co-fractionation Mus musculus
37 Rps15a 267019
Co-fractionation Mus musculus
38 Rock2  
Co-fractionation Mus musculus
39 Poldip3  
Co-fractionation Mus musculus
40 Rps20 67427
Co-fractionation Mus musculus
41 Dhx9 13211
Co-fractionation Mus musculus
42 Rpl30 19946
Co-fractionation Mus musculus
43 Rpl39 67248
Co-fractionation Mus musculus
44 Rps16 20055
Co-fractionation Mus musculus
45 Rps17 20068
Co-fractionation Mus musculus
46 Rps18 20084
Co-fractionation Mus musculus
47 Rpl13a 22121
Co-fractionation Mus musculus
48 Rpl14 67115
Co-fractionation Mus musculus
49 Rps2 16898
Co-fractionation Mus musculus
50 Gapdh 14433
Co-fractionation Mus musculus
51 Rps8 20116
Co-fractionation Mus musculus
52 Rpl7a 27176
Co-fractionation Mus musculus
53 Rpl32 19951
Co-fractionation Mus musculus
54 Rps28 54127
Co-fractionation Mus musculus
55 Rps19 20085
Co-fractionation Mus musculus
56 Tpt1 22070
Co-fractionation Mus musculus
57 Rps11 27207
Co-fractionation Mus musculus
58 Rpl18a 76808
Co-fractionation Mus musculus
59 Ywhae 22627
Affinity Capture-MS Mus musculus
60 Pcbp1 23983
Co-fractionation Mus musculus
61 Rpl22 19934
Co-fractionation Mus musculus
62 Rps6 20104
Co-fractionation Mus musculus
63 Hnrnpab 15384
Co-fractionation Mus musculus
64 Rpl38  
Co-fractionation Mus musculus
65 Syncrip 56403
Co-fractionation Mus musculus
66 Rps3a1 20091
Co-fractionation Mus musculus
67 Rps14 20044
Co-fractionation Mus musculus
68 Rpl35 66489
Co-fractionation Mus musculus
69 Rpl5 100503670
Co-fractionation Mus musculus
70 Fancd2  
Affinity Capture-MS Mus musculus
71 Rps27a 78294
Co-fractionation Mus musculus
72 Ncbp1 433702
Co-fractionation Mus musculus
73 Rps25 75617
Co-fractionation Mus musculus
74 Eef1e1  
Co-fractionation Mus musculus
75 Rpl10a 19896
Co-fractionation Mus musculus
76 Hnrnpl 15388
Co-fractionation Mus musculus
77 Kctd13  
Affinity Capture-MS Mus musculus
78 Rpgrip1l  
Affinity Capture-MS Mus musculus
79 Ddx6 13209
Co-fractionation Mus musculus
80 Fzr1  
Affinity Capture-MS Mus musculus
81 Eef2 13629
Co-fractionation Mus musculus
82 Pa2g4 18813
Co-fractionation Mus musculus
83 Rpl12 269261
Co-fractionation Mus musculus
84 Rpl18 19899
Co-fractionation Mus musculus
85 Gnb2l1 14694
Co-fractionation Mus musculus
86 Rps15 20054
Co-fractionation Mus musculus
87 Rps13 68052
Co-fractionation Mus musculus
88 Hnrnpa2b1 53379
Co-fractionation Mus musculus
89 Rpl23 65019
Co-fractionation Mus musculus
90 Rpl23a 268449
Co-fractionation Mus musculus
91 Rpl15 66480
Co-fractionation Mus musculus
92 Eef1b2 55949
Co-fractionation Mus musculus
93 Rpl4 67891
Co-fractionation Mus musculus
94 Rpl13 270106
Co-fractionation Mus musculus
95 Rpl8 26961
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here