Gene description for Calr
Gene name calreticulin
Gene symbol Calr
Other names/aliases CRT
Calregulin
Species Mus musculus
 Database cross references - Calr
ExoCarta ExoCarta_12317
Vesiclepedia VP_12317
Entrez Gene 12317
UniProt P14211  
 Calr identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Calr
Molecular Function
    complement component C1q complex binding GO:0001849 IEA
    complement component C1q complex binding GO:0001849 ISO
    mRNA binding GO:0003729 IDA
    mRNA binding GO:0003729 ISO
    nuclear export signal receptor activity GO:0005049 IEA
    nuclear export signal receptor activity GO:0005049 ISO
    integrin binding GO:0005178 IEA
    integrin binding GO:0005178 ISO
    iron ion binding GO:0005506 IEA
    iron ion binding GO:0005506 ISO
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    carbohydrate binding GO:0030246 IDA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    peptide binding GO:0042277 IEA
    peptide binding GO:0042277 ISO
    hormone binding GO:0042562 IEA
    hormone binding GO:0042562 ISO
    nuclear androgen receptor binding GO:0050681 IEA
    nuclear androgen receptor binding GO:0050681 ISO
    unfolded protein binding GO:0051082 IEA
    molecular sequestering activity GO:0140313 IEA
    molecular sequestering activity GO:0140313 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    peptide antigen assembly with MHC class I protein complex GO:0002502 IMP
    peptide antigen assembly with MHC class I protein complex GO:0002502 ISO
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IEA
    protein export from nucleus GO:0006611 IEA
    protein export from nucleus GO:0006611 ISO
    spermatogenesis GO:0007283 IEA
    positive regulation of cell population proliferation GO:0008284 IEA
    positive regulation of cell population proliferation GO:0008284 ISO
    response to xenobiotic stimulus GO:0009410 IEA
    positive regulation of endothelial cell migration GO:0010595 IEA
    positive regulation of endothelial cell migration GO:0010595 ISO
    positive regulation of gene expression GO:0010628 IMP
    negative regulation of translation GO:0017148 IEA
    negative regulation of translation GO:0017148 ISO
    cortical actin cytoskeleton organization GO:0030866 IDA
    response to estradiol GO:0032355 IEA
    negative regulation of intracellular steroid hormone receptor signaling pathway GO:0033144 IEA
    negative regulation of intracellular steroid hormone receptor signaling pathway GO:0033144 ISO
    response to testosterone GO:0033574 IEA
    protein localization to nucleus GO:0034504 IMP
    protein localization to nucleus GO:0034504 ISO
    ERAD pathway GO:0036503 IBA
    regulation of meiotic nuclear division GO:0040020 IDA
    negative regulation of neuron differentiation GO:0045665 IEA
    negative regulation of neuron differentiation GO:0045665 ISO
    positive regulation of cell cycle GO:0045787 IMP
    positive regulation of cell cycle GO:0045787 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of retinoic acid receptor signaling pathway GO:0048387 IEA
    negative regulation of retinoic acid receptor signaling pathway GO:0048387 ISO
    positive regulation of phagocytosis GO:0050766 IMP
    protein stabilization GO:0050821 IDA
    cardiac muscle cell differentiation GO:0055007 IEA
    cellular response to electrical stimulus GO:0071257 IEA
    cellular response to lithium ion GO:0071285 IEA
    cellular senescence GO:0090398 IMP
    cellular senescence GO:0090398 ISO
    cellular response to virus GO:0098586 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    negative regulation of trophoblast cell migration GO:1901164 IEA
    negative regulation of trophoblast cell migration GO:1901164 ISO
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 IMP
    response to peptide GO:1901652 IEA
    response to glycoside GO:1903416 IEA
    response to biphenyl GO:1904614 IEA
    positive regulation of dendritic cell chemotaxis GO:2000510 IEA
    positive regulation of dendritic cell chemotaxis GO:2000510 ISO
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    acrosomal vesicle GO:0001669 ISO
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 IEA
    nuclear envelope GO:0005635 ISO
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum lumen GO:0005788 IDA
    endoplasmic reticulum lumen GO:0005788 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    smooth endoplasmic reticulum GO:0005790 IEA
    smooth endoplasmic reticulum GO:0005790 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    ribosome GO:0005840 IEA
    ribosome GO:0005840 ISO
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 ISO
    membrane GO:0016020 ISO
    sarcoplasmic reticulum GO:0016529 ISO
    protein-containing complex GO:0032991 ISO
    sarcoplasmic reticulum lumen GO:0033018 IEA
    MHC class I peptide loading complex GO:0042824 IMP
    MHC class I peptide loading complex GO:0042824 ISO
    cytolytic granule GO:0044194 IEA
    endoplasmic reticulum quality control compartment GO:0044322 IDA
    phagocytic vesicle GO:0045335 TAS
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    cortical granule GO:0060473 ISS
    collagen-containing extracellular matrix GO:0062023 IEA
    collagen-containing extracellular matrix GO:0062023 ISO
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified Calr in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Calr
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Gabarap  
Protein-peptide Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 App 11820
Affinity Capture-MS Mus musculus
5 Sdf2l1  
Co-fractionation Mus musculus
6 Lipc  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
7 Olfm1  
Affinity Capture-MS Mus musculus
8 Tfe3  
Affinity Capture-MS Mus musculus
9 Tpd52l2  
Co-fractionation Mus musculus
10 Rps4x 20102
Co-fractionation Mus musculus
11 Psmc5 19184
Co-fractionation Mus musculus
12 Kcnma1  
Affinity Capture-MS Mus musculus
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