Gene description for Rplp2
Gene name ribosomal protein, large P2
Gene symbol Rplp2
Other names/aliases 2700049I22Rik
Species Mus musculus
 Database cross references - Rplp2
ExoCarta ExoCarta_67186
Vesiclepedia VP_67186
Entrez Gene 67186
UniProt P99027  
 Rplp2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rplp2
Molecular Function
    structural constituent of ribosome GO:0003735 IEA
    iron ion binding GO:0005506 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    cytoplasmic translational elongation GO:0002182 IEA
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rplp2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rplp2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl28 19943
Co-fractionation Mus musculus
4 Rpl35 66489
Co-fractionation Mus musculus
5 Rplp0 11837
Co-fractionation Mus musculus
6 Rpl31 114641
Co-fractionation Mus musculus
7 Atp5j  
Co-fractionation Mus musculus
8 Tubgcp4 51885
Co-fractionation Mus musculus
9 Rps25 75617
Co-fractionation Mus musculus
10 Rps26 27370
Co-fractionation Mus musculus
11 Rpl13a 22121
Co-fractionation Mus musculus
12 Tubgcp2  
Co-fractionation Mus musculus
13 Eif5a 276770
Co-fractionation Mus musculus
14 Rpl15 66480
Co-fractionation Mus musculus
15 Rpl38  
Co-fractionation Mus musculus
16 Rps4x 20102
Co-fractionation Mus musculus
17 Rpl4 67891
Co-fractionation Mus musculus
18 Rpl14 67115
Co-fractionation Mus musculus
19 Rps2 16898
Co-fractionation Mus musculus
20 Rpl11 67025
Co-fractionation Mus musculus
21 Rps10 67097
Co-fractionation Mus musculus
22 Rps8 20116
Co-fractionation Mus musculus
23 Rps23 66475
Co-fractionation Mus musculus
24 Rpl7a 27176
Co-fractionation Mus musculus
25 Rps18 20084
Co-fractionation Mus musculus
26 Rpl27 19942
Co-fractionation Mus musculus
27 Rpl32 19951
Co-fractionation Mus musculus
28 Pa2g4 18813
Co-fractionation Mus musculus
29 Fzr1  
Affinity Capture-MS Mus musculus
30 Rps19 20085
Co-fractionation Mus musculus
31 Tfe3  
Affinity Capture-MS Mus musculus
32 Rpl9 20005
Co-fractionation Mus musculus
33 Rpl24 68193
Co-fractionation Mus musculus
34 Tpt1 22070
Co-fractionation Mus musculus
35 Rps11 27207
Co-fractionation Mus musculus
36 Rpl18a 76808
Co-fractionation Mus musculus
37 Rps24 20088
Co-fractionation Mus musculus
38 Pabpc1 18458
Co-fractionation Mus musculus
39 Rps5 20103
Co-fractionation Mus musculus
40 Rps7 20115
Co-fractionation Mus musculus
41 Rpl17 319195
Co-fractionation Mus musculus
42 Rpl22 19934
Co-fractionation Mus musculus
43 Rps16 20055
Co-fractionation Mus musculus
44 Eif6 16418
Co-fractionation Mus musculus
45 Eef2 13629
Co-fractionation Mus musculus
46 Rps20 67427
Co-fractionation Mus musculus
47 Raver1  
Co-fractionation Mus musculus
48 Ppia 268373
Co-fractionation Mus musculus
49 Rpl12 269261
Co-fractionation Mus musculus
50 Rpl18 19899
Co-fractionation Mus musculus
51 Rpl21 19933
Co-fractionation Mus musculus
52 Rps13 68052
Co-fractionation Mus musculus
53 Rps21 66481
Co-fractionation Mus musculus
54 Rpl7 19989
Co-fractionation Mus musculus
55 Rps15a 267019
Co-fractionation Mus musculus
56 Gnb2l1 14694
Co-fractionation Mus musculus
57 Rps15 20054
Co-fractionation Mus musculus
58 Eef1a1 13627
Co-fractionation Mus musculus
59 Rps3a1 20091
Co-fractionation Mus musculus
60 Rpl36a 19982
Co-fractionation Mus musculus
61 Rps9 76846
Co-fractionation Mus musculus
62 Rpl26 19941
Co-fractionation Mus musculus
63 Fancd2  
Affinity Capture-MS Mus musculus
64 Rpl23 65019
Co-fractionation Mus musculus
65 Rpl23a 268449
Co-fractionation Mus musculus
66 Rps14 20044
Co-fractionation Mus musculus
67 Rps17 20068
Co-fractionation Mus musculus
68 Rps6 20104
Co-fractionation Mus musculus
69 Rpl30 19946
Co-fractionation Mus musculus
70 Eed  
Affinity Capture-MS Mus musculus
71 Rpsa 16785
Co-fractionation Mus musculus
72 Rpl8 26961
Co-fractionation Mus musculus
73 Eef1b2 55949
Co-fractionation Mus musculus
74 Rpl3 27367
Co-fractionation Mus musculus
75 Atg16l1  
Affinity Capture-MS Mus musculus
76 Rpl5 100503670
Co-fractionation Mus musculus
77 Rpl13 270106
Co-fractionation Mus musculus
78 Rpl6 19988
Co-fractionation Mus musculus
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