Gene description for Rpl7a
Gene name ribosomal protein L7A
Gene symbol Rpl7a
Other names/aliases Surf3
Species Mus musculus
 Database cross references - Rpl7a
ExoCarta ExoCarta_27176
Vesiclepedia VP_27176
Entrez Gene 27176
UniProt P12970  
 Rpl7a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl7a
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
Biological Process
    maturation of LSU-rRNA GO:0000470 IBA
    cytoplasmic translation GO:0002181 NAS
    ribosome biogenesis GO:0042254 IEA
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IDA
    ribosome GO:0005840 ISO
    ribosome GO:0005840 NAS
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl7a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl7a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Ola1 67059
Co-fractionation Mus musculus
4 Lars 107045
Co-fractionation Mus musculus
5 Rpl27 19942
Co-fractionation Mus musculus
6 Rpl6 19988
Co-fractionation Mus musculus
7 Rps24 20088
Co-fractionation Mus musculus
8 Tmem173  
Proximity Label-MS Mus musculus
9 Rpl7 19989
Co-fractionation Mus musculus
10 Eef1a1 13627
Co-fractionation Mus musculus
11 Plod3 26433
Co-fractionation Mus musculus
12 Atp9a  
Co-fractionation Mus musculus
13 Rpl36a 19982
Co-fractionation Mus musculus
14 Rpl26 19941
Co-fractionation Mus musculus
15 Rpsa 16785
Co-fractionation Mus musculus
16 Rpl3 27367
Co-fractionation Mus musculus
17 Abce1 24015
Co-fractionation Mus musculus
18 Rpl11 67025
Co-fractionation Mus musculus
19 Eed  
Affinity Capture-MS Mus musculus
20 Elf5  
Affinity Capture-MS Mus musculus
21 Rpl31 114641
Co-fractionation Mus musculus
22 Rps10 67097
Co-fractionation Mus musculus
23 Rplp2 67186
Co-fractionation Mus musculus
24 Rps4x 20102
Co-fractionation Mus musculus
25 Rps23 66475
Co-fractionation Mus musculus
26 Scyl2  
Co-fractionation Mus musculus
27 Tfe3  
Affinity Capture-MS Mus musculus
28 Rpl24 68193
Co-fractionation Mus musculus
29 Naa35  
Co-fractionation Mus musculus
30 Rps7 20115
Co-fractionation Mus musculus
31 Rpl17 319195
Co-fractionation Mus musculus
32 Rps5 20103
Co-fractionation Mus musculus
33 Dicer1  
Co-fractionation Mus musculus
34 Rps15a 267019
Co-fractionation Mus musculus
35 Ppwd1  
Co-fractionation Mus musculus
36 Fancd2  
Affinity Capture-MS Mus musculus
37 Rplp0 11837
Co-fractionation Mus musculus
38 Rpl30 19946
Co-fractionation Mus musculus
39 Rpl39 67248
Co-fractionation Mus musculus
40 Rps16 20055
Co-fractionation Mus musculus
41 Rps17 20068
Co-fractionation Mus musculus
42 Tcf3  
Affinity Capture-MS Mus musculus
43 Sf3a3 75062
Co-fractionation Mus musculus
44 Dhx29  
Co-fractionation Mus musculus
45 Rps18 20084
Co-fractionation Mus musculus
46 Rpl13a 22121
Co-fractionation Mus musculus
47 Rpl14 67115
Co-fractionation Mus musculus
48 Rps2 16898
Co-fractionation Mus musculus
49 Rps8 20116
Co-fractionation Mus musculus
50 Rpl32 19951
Co-fractionation Mus musculus
51 Rps19 20085
Co-fractionation Mus musculus
52 Tpt1 22070
Co-fractionation Mus musculus
53 Rpl18a 76808
Co-fractionation Mus musculus
54 Lrrc59  
Co-fractionation Mus musculus
55 Rpl22 19934
Co-fractionation Mus musculus
56 Rpl38  
Co-fractionation Mus musculus
57 Rps21 66481
Co-fractionation Mus musculus
58 Rps14 20044
Co-fractionation Mus musculus
59 Rpl35 66489
Co-fractionation Mus musculus
60 Dhfr 13361
Co-fractionation Mus musculus
61 Rpl5 100503670
Co-fractionation Mus musculus
62 Rps20 67427
Co-fractionation Mus musculus
63 Usp46  
Co-fractionation Mus musculus
64 Rps27a 78294
Co-fractionation Mus musculus
65 Ebna1bp2  
Co-fractionation Mus musculus
66 Rps25 75617
Co-fractionation Mus musculus
67 Upf1 19704
Co-fractionation Mus musculus
68 Rpl10a 19896
Co-fractionation Mus musculus
69 Eef1b2 55949
Co-fractionation Mus musculus
70 Atxn1  
Proximity Label-MS Mus musculus
71 Kctd13  
Affinity Capture-MS Mus musculus
72 Fzr1  
Affinity Capture-MS Mus musculus
73 Rpl12 269261
Co-fractionation Mus musculus
74 Rpl18 19899
Co-fractionation Mus musculus
75 Gnb2l1 14694
Co-fractionation Mus musculus
76 Rps15 20054
Co-fractionation Mus musculus
77 Rps9 76846
Co-fractionation Mus musculus
78 Rps13 68052
Co-fractionation Mus musculus
79 Tcf25  
Co-fractionation Mus musculus
80 Rpl23 65019
Co-fractionation Mus musculus
81 Rpl23a 268449
Co-fractionation Mus musculus
82 Rpl15 66480
Co-fractionation Mus musculus
83 Top1 21969
Co-fractionation Mus musculus
84 Rpl4 67891
Co-fractionation Mus musculus
85 Rpl13 270106
Co-fractionation Mus musculus
86 Rpl8 26961
Co-fractionation Mus musculus
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