Gene description for Eef2
Gene name eukaryotic translation elongation factor 2
Gene symbol Eef2
Other names/aliases Ef-2
Species Mus musculus
 Database cross references - Eef2
ExoCarta ExoCarta_13629
Vesiclepedia VP_13629
Entrez Gene 13629
UniProt P58252  
 Eef2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eef2
Molecular Function
    p53 binding GO:0002039 ISO
    RNA binding GO:0003723 ISO
    translation elongation factor activity GO:0003746 IBA
    translation elongation factor activity GO:0003746 ISO
    translation elongation factor activity GO:0003746 ISS
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    5S rRNA binding GO:0008097 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    ribosome binding GO:0043022 IBA
    ribosome binding GO:0043022 IEA
    ribosome binding GO:0043022 ISO
    actin filament binding GO:0051015 ISO
    lncRNA binding GO:0106222 IDA
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IMP
    translational elongation GO:0006414 IBA
    translational elongation GO:0006414 IDA
    translational elongation GO:0006414 ISO
    positive regulation of translation GO:0045727 IEA
    positive regulation of translation GO:0045727 ISO
    translation at postsynapse GO:0140242 ISO
    positive regulation of cytoplasmic translation GO:2000767 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    ribosome GO:0005840 IDA
    ribosome GO:0005840 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    aggresome GO:0016235 IEA
    aggresome GO:0016235 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    glutamatergic synapse GO:0098978 ISO
    ribonucleoprotein complex GO:1990904 IBA
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Eef2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
8
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
9
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eef2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rpl11 67025
Co-fractionation Mus musculus
3 Rpl39 67248
Co-fractionation Mus musculus
4 Rplp2 67186
Co-fractionation Mus musculus
5 Rpl31 114641
Co-fractionation Mus musculus
6 CUL1 8454
Affinity Capture-MS Homo sapiens
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 Rpl13a 22121
Co-fractionation Mus musculus
9 TFIP11  
Two-hybrid Homo sapiens
10 Pou5f1  
Affinity Capture-MS Mus musculus
11 Rpl38  
Co-fractionation Mus musculus
12 Rps4x 20102
Co-fractionation Mus musculus
13 Rpl14 67115
Co-fractionation Mus musculus
14 Rps2 16898
Co-fractionation Mus musculus
15 Rps23 66475
Co-fractionation Mus musculus
16 Tsc1  
Affinity Capture-MS Mus musculus
17 Cul3  
Affinity Capture-MS Mus musculus
18 Rpl32 19951
Co-fractionation Mus musculus
19 DMRTB1  
Two-hybrid Homo sapiens
20 Rps28 54127
Co-fractionation Mus musculus
21 Kctd13  
Affinity Capture-MS Mus musculus
22 Rps19 20085
Co-fractionation Mus musculus
23 Rps3a1 20091
Co-fractionation Mus musculus
24 Tfe3  
Affinity Capture-MS Mus musculus
25 Rpl9 20005
Co-fractionation Mus musculus
26 Tpt1 22070
Co-fractionation Mus musculus
27 Rps6 20104
Co-fractionation Mus musculus
28 Rpl18a 76808
Co-fractionation Mus musculus
29 Iqcb1  
Affinity Capture-MS Mus musculus
30 Rps25 75617
Co-fractionation Mus musculus
31 Mettl21e  
Affinity Capture-MS Mus musculus
32 Tmem173  
Proximity Label-MS Mus musculus
33 Upf1 19704
Co-fractionation Mus musculus
34 Eef2 13629
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
35 Cbx5  
Affinity Capture-MS Mus musculus
36 Traf6  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
37 Rpl18 19899
Co-fractionation Mus musculus
38 Rps13 68052
Co-fractionation Mus musculus
39 Rps21 66481
Co-fractionation Mus musculus
40 Rpl23 65019
Co-fractionation Mus musculus
41 Unk  
Affinity Capture-RNA Mus musculus
42 Gnb2l1 14694
Co-fractionation Mus musculus
43 Rps15 20054
Co-fractionation Mus musculus
44 Rps5 20103
Co-fractionation Mus musculus
45 Huwe1  
Affinity Capture-MS Mus musculus
46 Npm1 18148
Affinity Capture-MS Mus musculus
47 Raver1  
Co-fractionation Mus musculus
48 Rps17 20068
Co-fractionation Mus musculus
49 Rplp0 11837
Co-fractionation Mus musculus
50 Rpl23a 268449
Co-fractionation Mus musculus
51 Rps14 20044
Co-fractionation Mus musculus
52 Eed  
Affinity Capture-MS Mus musculus
53 Rpl15 66480
Co-fractionation Mus musculus
54 Rps18 20084
Co-fractionation Mus musculus
55 Atg16l1  
Affinity Capture-MS Mus musculus
56 Nphp1  
Affinity Capture-MS Mus musculus
57 Rpl4 67891
Co-fractionation Mus musculus
58 Fancd2  
Affinity Capture-MS Mus musculus
59 Rpl8 26961
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here