Gene description for Plod3
Gene name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Gene symbol Plod3
Other names/aliases AI414586
LH3
Species Mus musculus
 Database cross references - Plod3
ExoCarta ExoCarta_26433
Vesiclepedia VP_26433
Entrez Gene 26433
UniProt Q9R0E1  
 Plod3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plod3
Molecular Function
    iron ion binding GO:0005506 IEA
    iron ion binding GO:0005506 ISO
    procollagen-lysine 5-dioxygenase activity GO:0008475 IBA
    procollagen-lysine 5-dioxygenase activity GO:0008475 IDA
    procollagen-lysine 5-dioxygenase activity GO:0008475 ISO
    L-ascorbic acid binding GO:0031418 IEA
    procollagen glucosyltransferase activity GO:0033823 IBA
    procollagen glucosyltransferase activity GO:0033823 IDA
    procollagen glucosyltransferase activity GO:0033823 ISO
    small molecule binding GO:0036094 ISO
    metal ion binding GO:0046872 ISO
    procollagen galactosyltransferase activity GO:0050211 ISO
    procollagen galactosyltransferase activity GO:0050211 ISS
Biological Process
    in utero embryonic development GO:0001701 IMP
    endothelial cell morphogenesis GO:0001886 IMP
    protein O-linked glycosylation GO:0006493 IEA
    protein O-linked glycosylation GO:0006493 ISO
    protein localization GO:0008104 IMP
    peptidyl-lysine hydroxylation GO:0017185 IDA
    peptidyl-lysine hydroxylation GO:0017185 IMP
    peptidyl-lysine hydroxylation GO:0017185 ISO
    peptidyl-lysine hydroxylation GO:0017185 ISS
    neural tube development GO:0021915 IMP
    collagen fibril organization GO:0030199 IBA
    collagen fibril organization GO:0030199 IMP
    collagen metabolic process GO:0032963 IMP
    vasodilation GO:0042311 IMP
    hydroxylysine biosynthetic process GO:0046947 IDA
    epidermis morphogenesis GO:0048730 IMP
    lung morphogenesis GO:0060425 IMP
    basement membrane assembly GO:0070831 IMP
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    rough endoplasmic reticulum GO:0005791 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 IDA
 Experiment description of studies that identified Plod3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plod3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpl35a 57808
Co-fractionation Mus musculus
2 Lrrc59  
Co-fractionation Mus musculus
3 Rpl22 19934
Co-fractionation Mus musculus
4 Dhx29  
Co-fractionation Mus musculus
5 Mettl21e  
Affinity Capture-MS Mus musculus
6 Rps14 20044
Co-fractionation Mus musculus
7 Atxn1  
Affinity Capture-MS Mus musculus
8 Rpl6 19988
Co-fractionation Mus musculus
9 Eed  
Affinity Capture-MS Mus musculus
10 Gtf2f1  
Co-fractionation Mus musculus
11 Dhfr 13361
Co-fractionation Mus musculus
12 Sf3a3 75062
Co-fractionation Mus musculus
13 Rpl7a 27176
Co-fractionation Mus musculus
14 Rps6 20104
Co-fractionation Mus musculus
15 Mlec  
Co-fractionation Mus musculus
16 Zfp330  
Co-fractionation Mus musculus
17 Rps4x 20102
Co-fractionation Mus musculus
18 Rcsd1  
Co-fractionation Mus musculus
19 Rps3a1 20091
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Plod3 is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes IEA Reactome
Collagen formation IEA Reactome
Extracellular matrix organization IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here