Gene description for ACY1
Gene name aminoacylase 1
Gene symbol ACY1
Other names/aliases ACY-1
ACY1D
HEL-S-5
Species Homo sapiens
 Database cross references - ACY1
ExoCarta ExoCarta_95
Vesiclepedia VP_95
Entrez Gene 95
HGNC 177
MIM 104620
UniProt Q03154  
 ACY1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Urine 15326289    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ACY1
Molecular Function
    aminoacylase activity GO:0004046 IBA
    aminoacylase activity GO:0004046 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    amino acid metabolic process GO:0006520 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified ACY1 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
4
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACY1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSTP1 2950
Affinity Capture-MS Homo sapiens
2 VPS28 51160
Affinity Capture-MS Homo sapiens
3 MAPKAPK2 9261
Co-fractionation Homo sapiens
4 DHFR 1719
Co-fractionation Homo sapiens
5 PRR35  
Two-hybrid Homo sapiens
6 BCAS2 10286
Two-hybrid Homo sapiens
7 SOD1 6647
Co-fractionation Homo sapiens
8 POT1  
Two-hybrid Homo sapiens
9 SEC13 6396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PHOSPHO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 BOLL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GPR182  
Affinity Capture-MS Homo sapiens
13 ARL6IP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TRIM33 51592
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FAM46B  
Two-hybrid Homo sapiens
16 TLX3  
Two-hybrid Homo sapiens
17 WWOX 51741
Two-hybrid Homo sapiens
18 IQCF1  
Affinity Capture-MS Homo sapiens
19 BPNT1 10380
Co-fractionation Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 POU2F1 5451
Co-fractionation Homo sapiens
22 RAB4A 5867
Affinity Capture-MS Homo sapiens
23 GLO1 2739
Co-fractionation Homo sapiens
24 TERF1 7013
Two-hybrid Homo sapiens
25 PECR 55825
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NUDT1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
27 RNF166  
Affinity Capture-MS Homo sapiens
28 AP4M1 9179
Affinity Capture-MS Homo sapiens
29 PPP1R21 129285
Affinity Capture-MS Homo sapiens
30 TTC1 7265
Two-hybrid Homo sapiens
31 HYI 81888
Co-fractionation Homo sapiens
32 NPM1 4869
Two-hybrid Homo sapiens
33 P2RY10  
Affinity Capture-MS Homo sapiens
34 ACY1 95
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
35 IMPA1 3612
Co-fractionation Homo sapiens
36 PDRG1  
Co-fractionation Homo sapiens
37 ICOS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 PPME1 51400
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 AR 367
Affinity Capture-MS Homo sapiens
40 WDYHV1  
Two-hybrid Homo sapiens
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