|
 Gene ontology annotations for CD63 |
|
|
| Experiment description of studies that identified CD63 in sEVs |
| 1 |
| Experiment ID |
11 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
HSC70|HSP90|MHCII|CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
12519789
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. |
| Authors |
"Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W" |
| Journal name |
JBC
|
| Publication year |
2003 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15+) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.15 g/mL
|
| Molecules identified in the study |
Protein
Lipids |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Mass spectrometry [QTOF]
Western blotting
Thin layer chromatography
HPLC |
|
|
| 2 |
| Experiment ID |
16 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
9685355
|
| Organism |
Homo sapiens |
| Experiment description |
Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. |
| Authors |
"Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ" |
| Journal name |
JBC
|
| Publication year |
1998 |
| Sample |
B cells |
| Sample name |
RN (HLA-DR15+) |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.12-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunoelectron Microscopy |
|
|
| 3 |
| Experiment ID |
47 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|CD86
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
|
| PubMed ID |
17641064
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with immune modulatory features are present in human breast milk. |
| Authors |
"Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" |
| Journal name |
JIMMU
|
| Publication year |
2007 |
| Sample |
B cells |
| Sample name |
B cells |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS |
|
|
| 4 |
| Experiment ID |
116 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21505438
|
| Organism |
Homo sapiens |
| Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
| Authors |
"Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÃÂ, Bernad A, Sánchez-Madrid F." |
| Journal name |
NATCOM
|
| Publication year |
2011 |
| Sample |
B cells |
| Sample name |
Raji |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
Microarray |
|
|
| 5 |
| Experiment ID |
76 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
|
| PubMed ID |
20224111
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomics analysis of bladder cancer exosomes. |
| Authors |
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A." |
| Journal name |
MCP
|
| Publication year |
2010 |
| Sample |
Bladder cancer cells |
| Sample name |
HT1376 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS |
|
|
| 6 |
| Experiment ID |
42 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
15908444
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal-like vesicles are present in human blood plasma. |
| Authors |
"Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C" |
| Journal name |
INTIMM
|
| Publication year |
2005 |
| Sample |
Bone marrow cells |
| Sample name |
PBMC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 7 |
| Experiment ID |
489 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 8 |
| Experiment ID |
490 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 9 |
| Experiment ID |
491 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 10 |
| Experiment ID |
492 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 11 |
| Experiment ID |
758 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✔
CANX|GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35351860
|
| Organism |
Homo sapiens |
| Experiment description |
Bone marrow mesenchymal stem cell-derived exosomal microRNA-381-3p alleviates vascular calcification in chronic kidney disease by targeting NFAT5 |
| Authors |
"Liu Y, Guo Y, Bao S, Huang H, Liu W, Guo W." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 12 |
| Experiment ID |
397 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 13 |
| Experiment ID |
397 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 14 |
| Experiment ID |
398 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 15 |
| Experiment ID |
398 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 16 |
| Experiment ID |
412 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 17 |
| Experiment ID |
414 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 18 |
| Experiment ID |
920 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 19 |
| Experiment ID |
921 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 20 |
| Experiment ID |
924 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 21 |
| Experiment ID |
925 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 22 |
| Experiment ID |
926 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 23 |
| Experiment ID |
927 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|SDCBP|CD81|RAB35|LAMP2|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 24 |
| Experiment ID |
930 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 25 |
| Experiment ID |
931 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 26 |
| Experiment ID |
932 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT1|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 27 |
| Experiment ID |
933 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|FLOT1|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 28 |
| Experiment ID |
942 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
BALB/c implanted with MDA-MB-231-luci (+) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 29 |
| Experiment ID |
943 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231-luci (+) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 30 |
| Experiment ID |
945 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
BALB/c implanted with MDA-MB-231-WT |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 31 |
| Experiment ID |
946 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
BALB/c implanted with MDA-MB-231-ETA KD |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 32 |
| Experiment ID |
989 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 33 |
| Experiment ID |
990 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 34 |
| Experiment ID |
1331 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|ICAM1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34796683
|
| Organism |
Homo sapiens |
| Experiment description |
High TSPAN8 expression in epithelial cancer cell-derived small extracellular vesicles promote confined diffusion and pronounced uptake |
| Authors |
"Wang T, Wang X, Wang H, Li L, Zhang C, Xiang R, Tan X, Li Z, Jiang C, Zheng L, Xiao L, Yue S." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
T-47D |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 35 |
| Experiment ID |
1332 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|ICAM1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34796683
|
| Organism |
Homo sapiens |
| Experiment description |
High TSPAN8 expression in epithelial cancer cell-derived small extracellular vesicles promote confined diffusion and pronounced uptake |
| Authors |
"Wang T, Wang X, Wang H, Li L, Zhang C, Xiang R, Tan X, Li Z, Jiang C, Zheng L, Xiao L, Yue S." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
T-47D |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 36 |
| Experiment ID |
46 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
HSC70|CD63|MHCII|CD81|CD86
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunoelectron Microscopy
FACS
|
| PubMed ID |
17641064
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with immune modulatory features are present in human breast milk. |
| Authors |
"Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" |
| Journal name |
JIMMU
|
| Publication year |
2007 |
| Sample |
Breast milk |
| Sample name |
Breast milk - Colostrum |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
1.10-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS |
|
|
| 37 |
| Experiment ID |
48 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
HSC70|CD63|CD86|MHCII
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunoelectron Microscopy
FACS
|
| PubMed ID |
17641064
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with immune modulatory features are present in human breast milk. |
| Authors |
"Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S" |
| Journal name |
JIMMU
|
| Publication year |
2007 |
| Sample |
Breast milk |
| Sample name |
Breast milk - Mature milk |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
1.10-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS |
|
|
| 38 |
| Experiment ID |
124 |
| MISEV standards |
|
✔
IEM|EM
|
Biophysical techniques |
|
✔
HSC70|CD63|CD9|CD81
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunoelectron microscopy
|
| PubMed ID |
21235781
|
| Organism |
Homo sapiens |
| Experiment description |
"Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages." |
| Authors |
"Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H." |
| Journal name |
JTM
|
| Publication year |
2011 |
| Sample |
Breast milk |
| Sample name |
Breast milk - Normal |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS
Immunoelectron Microscopy
Western blotting |
|
|
| 39 |
| Experiment ID |
49 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
CD63|CD86
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
Immunoelectron Microscopy
|
| PubMed ID |
14582906
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid. |
| Authors |
"Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S" |
| Journal name |
ERJ
|
| Publication year |
2003 |
| Sample |
Bronchoalveolar lavage fluid |
| Sample name |
Bronchoalveolar lavage fluid |
| Isolation/purification methods |
Differential centrifugation
Immunobeads (EpCAM) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS
Immunoelectron Microscopy |
|
|
| 40 |
| Experiment ID |
388 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33767144
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells |
| Authors |
"Bonsergent E, Grisard E, Buchrieser J, Schwartz O, Théry C, Lavieu G." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 41 |
| Experiment ID |
789 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 42 |
| Experiment ID |
790 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 43 |
| Experiment ID |
791 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 44 |
| Experiment ID |
792 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 45 |
| Experiment ID |
1325 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
immuno-EM
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immuno-EM |
|
|
| 46 |
| Experiment ID |
1326 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 47 |
| Experiment ID |
1327 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 48 |
| Experiment ID |
1328 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|SDCBP|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 49 |
| Experiment ID |
1329 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|SDCBP|LAMP1|CD81|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 50 |
| Experiment ID |
1330 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|SDCBP|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 51 |
| Experiment ID |
494 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Osteoarthritic cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 52 |
| Experiment ID |
496 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 53 |
| Experiment ID |
497 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 54 |
| Experiment ID |
498 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 55 |
| Experiment ID |
1265 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW480 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 56 |
| Experiment ID |
1266 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW480 - Oxaliplatin-resistant |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 57 |
| Experiment ID |
1267 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW620 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 58 |
| Experiment ID |
1268 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
SW620 - Oxaliplatin-resistant |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 59 |
| Experiment ID |
1269 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.05 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 60 |
| Experiment ID |
1270 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.06 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 61 |
| Experiment ID |
1271 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.08 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 62 |
| Experiment ID |
1272 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 63 |
| Experiment ID |
1273 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 8 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.12 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 64 |
| Experiment ID |
1274 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35165262
|
| Organism |
Homo sapiens |
| Experiment description |
DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer |
| Authors |
"Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colon cancer cells |
| Sample name |
COAD - Fraction 9 |
| Isolation/purification methods |
Differential centrifugation
Tangential flow filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 65 |
| Experiment ID |
20 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP90|CD63|CD81|LAMP1
|
Enriched markers |
|
✔
GOLGA2|cytochrome c
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
17956143
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
| Authors |
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" |
| Journal name |
JPR
|
| Publication year |
2007 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Diafiltration |
| Flotation density |
1.16 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| 66 |
| Experiment ID |
22 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
Immunoelectron Microscopy
FACS
|
| PubMed ID |
15940614
|
| Organism |
Homo sapiens |
| Experiment description |
Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape. |
| Authors |
"Huber V, Fais S, Iero M, Lugini L, Canese P, Squarcina P, Zaccheddu A, Colone M, Arancia G, Gentile M, Seregni E, Valenti R, Ballabio G, Belli F, Leo E, Parmiani G, Rivoltini L." |
| Journal name |
GASTRO
|
| Publication year |
2005 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW403
1869col
CRC28462 |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunoelectron Microscopy
FACS |
|
|
| 67 |
| Experiment ID |
201 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
22740476
|
| Organism |
Homo sapiens |
| Experiment description |
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4. |
| Authors |
"Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ." |
| Journal name |
Electrophoresis
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 68 |
| Experiment ID |
207 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DKO-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 69 |
| Experiment ID |
208 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
Dks-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 70 |
| Experiment ID |
209 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 71 |
| Experiment ID |
285 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 72 |
| Experiment ID |
286 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 73 |
| Experiment ID |
399 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 74 |
| Experiment ID |
399 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 75 |
| Experiment ID |
400 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 76 |
| Experiment ID |
400 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 77 |
| Experiment ID |
763 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35443745
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts |
| Authors |
"Wang D, Wang X, Song Y, Si M, Sun Y, Liu X, Cui S, Qu X, Yu X." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Ultracentrifugation
ExoQuick Kit |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RNA sequencing
RT-qPCR |
|
|
| 78 |
| Experiment ID |
764 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35443745
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts |
| Authors |
"Wang D, Wang X, Song Y, Si M, Sun Y, Liu X, Cui S, Qu X, Yu X." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Ultracentrifugation
ExoQuick Kit |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RNA sequencing
RT-qPCR |
|
|
| 79 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 80 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 81 |
| Experiment ID |
1340 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 82 |
| Experiment ID |
1341 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 83 |
| Experiment ID |
1342 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 84 |
| Experiment ID |
1343 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 85 |
| Experiment ID |
1344 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 86 |
| Experiment ID |
1345 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 87 |
| Experiment ID |
1361 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|FLOT1|LAMP1|CD24|EPCAM|Alix
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
MORPH
ELISA |
|
|
| 88 |
| Experiment ID |
1361 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|FLOT1|LAMP1|CD24|EPCAM|Alix
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
MORPH
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
MORPH
ELISA |
|
|
| 89 |
| Experiment ID |
1361 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|FLOT1|LAMP1|CD24|EPCAM|Alix
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
ELISA
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
MORPH
ELISA |
|
|
| 90 |
| Experiment ID |
1362 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
MORPH
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 91 |
| Experiment ID |
1362 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
ELISA
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 92 |
| Experiment ID |
50 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
CD63|CD87
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
FACS
Immunoelectron Microscopy
|
| PubMed ID |
14582906
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid. |
| Authors |
"Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S" |
| Journal name |
ERJ
|
| Publication year |
2003 |
| Sample |
Dendritic cells |
| Sample name |
Monocyte-derived dendritic cells |
| Isolation/purification methods |
Differential centrifugation
Immunobeads (EpCAM) |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS
Immunoelectron Microscopy |
|
|
| 93 |
| Experiment ID |
117 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21505438
|
| Organism |
Homo sapiens |
| Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
| Authors |
"Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÃÂ, Bernad A, Sánchez-Madrid F." |
| Journal name |
NATCOM
|
| Publication year |
2011 |
| Sample |
Dendritic cells |
| Sample name |
Dendritic cells |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
Microarray |
|
|
| 94 |
| Experiment ID |
395 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33523942
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal delivery of NF-κB inhibitor delays LPS-induced preterm birth and modulates fetal immune cell profile in mouse models |
| Authors |
"Sheller-Miller S, Radnaa E, Yoo JK, Kim E, Choi K, Kim Y, Kim YN, Richardson L, Choi C, Menon R." |
| Journal name |
Sci Adv
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Centrifugation
Filtration
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 95 |
| Experiment ID |
396 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33523942
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal delivery of NF-κB inhibitor delays LPS-induced preterm birth and modulates fetal immune cell profile in mouse models |
| Authors |
"Sheller-Miller S, Radnaa E, Yoo JK, Kim E, Choi K, Kim Y, Kim YN, Richardson L, Choi C, Menon R." |
| Journal name |
Sci Adv
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Centrifugation
Filtration
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 96 |
| Experiment ID |
407 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 97 |
| Experiment ID |
452 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 98 |
| Experiment ID |
453 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 99 |
| Experiment ID |
454 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 100 |
| Experiment ID |
455 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 101 |
| Experiment ID |
923 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 102 |
| Experiment ID |
1145 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34545097
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated stable epigenetic repression of HIV-1 |
| Authors |
"Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 103 |
| Experiment ID |
1146 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34545097
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated stable epigenetic repression of HIV-1 |
| Authors |
"Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 104 |
| Experiment ID |
1147 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34545097
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated stable epigenetic repression of HIV-1 |
| Authors |
"Shrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
Western blotting
RT-qPCR |
|
|
| 105 |
| Experiment ID |
1275 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 106 |
| Experiment ID |
1280 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 107 |
| Experiment ID |
1365 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
MORPH
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Epidermoid cancer cells |
| Sample name |
A431 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 108 |
| Experiment ID |
305 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
Immunofluorescence
|
| PubMed ID |
33431899
|
| Organism |
Homo sapiens |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Healthy volunteers |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
HPLC |
|
|
| 109 |
| Experiment ID |
306 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
Immunofluorescence
|
| PubMed ID |
33431899
|
| Organism |
Homo sapiens |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Type 2 diabetes patients |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
HPLC |
|
|
| 110 |
| Experiment ID |
383 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✔
PCNA
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33523480
|
| Organism |
Homo sapiens |
| Experiment description |
Human fibroblast-derived extracellular vesicles promote hair growth in cultured human hair follicles |
| Authors |
"Rajendran RL, Gangadaran P, Kwack MH, Oh JM, Hong CM, Sung YK, Lee J, Ahn BC." |
| Journal name |
FEBS Lett
|
| Publication year |
2021 |
| Sample |
Fibroblasts |
| Sample name |
CCD-1135-SK |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Flow cytometry |
|
|
| 111 |
| Experiment ID |
1220 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
31792209
|
| Organism |
Homo sapiens |
| Experiment description |
Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles |
| Authors |
"Xie X, Nie H, Zhou Y, Lian S, Mei H, Lu Y, Dong H, Li F, Li T, Li B, Wang J, Lin M, Wang C, Shao J, Gao Y, Chen J, Xie F, Jia L." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Fibroblasts |
| Sample name |
human embryonic lung fibroblast (HELF) |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Flow cytometry |
|
|
| 112 |
| Experiment ID |
405 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Foreskin fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 113 |
| Experiment ID |
760 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36581340
|
| Organism |
Homo sapiens |
| Experiment description |
"Rab27b, a Regulator of Exosome Secretion, Is Associated With Peritoneal Metastases in Gastric Cancer" |
| Authors |
"Nambara S, Masuda T, Hirose K, Hu Q, Tobo T, Ozato Y, Kurashige J, Hiraki Y, Hisamatsu Y, Iguchi T, Sugimachi K, Oki E, Yoshizumi T, Mimori K." |
| Journal name |
Cancer Genomics Proteomics
|
| Publication year |
2023 |
| Sample |
Gastric cancer cells |
| Sample name |
58As9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 114 |
| Experiment ID |
761 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36581340
|
| Organism |
Homo sapiens |
| Experiment description |
"Rab27b, a Regulator of Exosome Secretion, Is Associated With Peritoneal Metastases in Gastric Cancer" |
| Authors |
"Nambara S, Masuda T, Hirose K, Hu Q, Tobo T, Ozato Y, Kurashige J, Hiraki Y, Hisamatsu Y, Iguchi T, Sugimachi K, Oki E, Yoshizumi T, Mimori K." |
| Journal name |
Cancer Genomics Proteomics
|
| Publication year |
2023 |
| Sample |
Gastric cancer cells |
| Sample name |
58As9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 115 |
| Experiment ID |
762 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36581340
|
| Organism |
Homo sapiens |
| Experiment description |
"Rab27b, a Regulator of Exosome Secretion, Is Associated With Peritoneal Metastases in Gastric Cancer" |
| Authors |
"Nambara S, Masuda T, Hirose K, Hu Q, Tobo T, Ozato Y, Kurashige J, Hiraki Y, Hisamatsu Y, Iguchi T, Sugimachi K, Oki E, Yoshizumi T, Mimori K." |
| Journal name |
Cancer Genomics Proteomics
|
| Publication year |
2023 |
| Sample |
Gastric cancer cells |
| Sample name |
58As9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 116 |
| Experiment ID |
1363 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
MORPH
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN45 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 117 |
| Experiment ID |
1363 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
ELISA
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN45 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 118 |
| Experiment ID |
1364 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
MORPH
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Gastric cancer cells |
| Sample name |
SNU484 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 119 |
| Experiment ID |
1364 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
ELISA
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Gastric cancer cells |
| Sample name |
SNU484 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 120 |
| Experiment ID |
539 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35986010
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12 |
| Authors |
"Bao Z, Zhang N, Niu W, Mu M, Zhang X, Hu S, Niu C." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Glial cells |
| Sample name |
HEB |
| Isolation/purification methods |
Centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miRNA sequencing |
|
|
| 121 |
| Experiment ID |
401 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Glioblastoma |
| Sample name |
U87 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Flow cytometry |
|
|
| 122 |
| Experiment ID |
403 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Glioblastoma |
| Sample name |
U87 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Flow cytometry |
|
|
| 123 |
| Experiment ID |
272 |
| MISEV standards |
|
✔
AFM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25498500
|
| Organism |
Homo sapiens |
| Experiment description |
Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components. |
| Authors |
"Toda Y, Takata K, Nakagawa Y, Kawakami H, Fujioka S, Kobayashi K, Hattori Y, Kitamura Y, Akaji K, Ashihara E." |
| Journal name |
Biochem Biophys Res Commun
|
| Publication year |
2015 |
| Sample |
Glioblastoma cells |
| Sample name |
U251 |
| Isolation/purification methods |
Differential centrifugation
Ultracenrifugation
Sucrose density gradient |
| Flotation density |
1.16 g/mL
|
| Molecules identified in the study |
Lipids
Protein |
| Methods used in the study |
Thin layer chromatography
Western blotting |
|
|
| 124 |
| Experiment ID |
538 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35986010
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12 |
| Authors |
"Bao Z, Zhang N, Niu W, Mu M, Zhang X, Hu S, Niu C." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Glioblastoma cells |
| Sample name |
GSCs |
| Isolation/purification methods |
Centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miRNA sequencing |
|
|
| 125 |
| Experiment ID |
1367 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
MORPH
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Glioma cells |
| Sample name |
GLI36 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 126 |
| Experiment ID |
1367 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD24|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
ELISA
|
| PubMed ID |
36303008
|
| Organism |
Homo sapiens |
| Experiment description |
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples |
| Authors |
"Zhao H, Pan S, Natalia A, Wu X, Ong CJ, Teo MCC, So JBY, Shao H." |
| Journal name |
Nat Biomed Eng
|
| Publication year |
2023 |
| Sample |
Glioma cells |
| Sample name |
GLI36 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
MORPH
ELISA |
|
|
| 127 |
| Experiment ID |
515 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|TSG101
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36103526
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases |
| Authors |
"Wan T, Zhong J, Pan Q, Zhou T, Ping Y, Liu X." |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Hepatic stellate cells |
| Sample name |
LX-2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 128 |
| Experiment ID |
516 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36103526
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases |
| Authors |
"Wan T, Zhong J, Pan Q, Zhou T, Ping Y, Liu X." |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Hepatic stellate cells |
| Sample name |
LX-2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 129 |
| Experiment ID |
517 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36103526
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases |
| Authors |
"Wan T, Zhong J, Pan Q, Zhou T, Ping Y, Liu X." |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Hepatic stellate cells |
| Sample name |
LX-2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 130 |
| Experiment ID |
518 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36103526
|
| Organism |
Homo sapiens |
| Experiment description |
Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases |
| Authors |
"Wan T, Zhong J, Pan Q, Zhou T, Ping Y, Liu X." |
| Journal name |
Sci Adv
|
| Publication year |
2022 |
| Sample |
Hepatic stellate cells |
| Sample name |
LX-2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 131 |
| Experiment ID |
540 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|FLOT2|LAMP1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36192599
|
| Organism |
Homo sapiens |
| Experiment description |
HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma |
| Authors |
"Chen QT, Zhang ZY, Huang QL, Chen HZ, Hong WB, Lin T, Zhao WX, Wang XM, Ju CY, Wu LZ, Huang YY, Hou PP, Wang WJ, Zhou D, Deng X, Wu Q. " |
| Journal name |
Nat Metab
|
| Publication year |
2022 |
| Sample |
Hepatic stellate cells |
| Sample name |
LX-2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 132 |
| Experiment ID |
541 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|FLOT2|LAMP1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36192599
|
| Organism |
Homo sapiens |
| Experiment description |
HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma |
| Authors |
"Chen QT, Zhang ZY, Huang QL, Chen HZ, Hong WB, Lin T, Zhao WX, Wang XM, Ju CY, Wu LZ, Huang YY, Hou PP, Wang WJ, Zhou D, Deng X, Wu Q. " |
| Journal name |
Nat Metab
|
| Publication year |
2022 |
| Sample |
Hepatic stellate cells |
| Sample name |
LX-2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 133 |
| Experiment ID |
234 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✔
qNano
|
Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
HKCI-C3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 134 |
| Experiment ID |
4 |
| MISEV standards |
|
✔
IEM
|
Biophysical techniques |
|
✔
CD63|MHCI|MHCII
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
11487543
|
| Organism |
Homo sapiens |
| Experiment description |
Intestinal epithelial cells secrete exosome-like vesicles. |
| Authors |
"van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M" |
| Journal name |
Not applicable
|
| Publication year |
2001 |
| Sample |
Intestinal epithelial cells |
| Sample name |
HT29-19A
T84-DRB1*0401/CIITA |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF]
Western blotting |
|
|
| 135 |
| Experiment ID |
691 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
CSQT-2 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 136 |
| Experiment ID |
692 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
HCC-LM3 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 137 |
| Experiment ID |
693 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
HepG2 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 138 |
| Experiment ID |
694 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
MHCC-97L |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 139 |
| Experiment ID |
1308 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|CD81|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 140 |
| Experiment ID |
1309 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD63|CD81|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 141 |
| Experiment ID |
1310 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 6 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 142 |
| Experiment ID |
1311 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 7 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 143 |
| Experiment ID |
1312 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 8 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 144 |
| Experiment ID |
1313 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 9 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 145 |
| Experiment ID |
1314 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 6 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 146 |
| Experiment ID |
1315 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 7 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 147 |
| Experiment ID |
1316 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 8 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 148 |
| Experiment ID |
1317 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 - Fraction 9 |
| Isolation/purification methods |
Centrifugation
Centrifugal ultrafiltration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 149 |
| Experiment ID |
1318 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 150 |
| Experiment ID |
1319 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32082513
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles |
| Authors |
"Jiang W, Ma P, Deng L, Liu Z, Wang X, Liu X, Long G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2020 |
| Sample |
Liver cancer cells |
| Sample name |
Huh-7 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 151 |
| Experiment ID |
1371 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
PLC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 152 |
| Experiment ID |
1372 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
PLC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 153 |
| Experiment ID |
1373 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 154 |
| Experiment ID |
1374 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 155 |
| Experiment ID |
1375 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 156 |
| Experiment ID |
1376 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 157 |
| Experiment ID |
1377 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 158 |
| Experiment ID |
1378 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 159 |
| Experiment ID |
1379 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immuno-EM
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immuno-EM |
|
|
| 160 |
| Experiment ID |
1380 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immuno-EM
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immuno-EM |
|
|
| 161 |
| Experiment ID |
1381 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 162 |
| Experiment ID |
1382 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD81|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 163 |
| Experiment ID |
328 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Flow cytometry |
|
|
| 164 |
| Experiment ID |
329 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Flow cytometry |
|
|
| 165 |
| Experiment ID |
542 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 166 |
| Experiment ID |
543 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 167 |
| Experiment ID |
544 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 168 |
| Experiment ID |
545 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299-fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 169 |
| Experiment ID |
546 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|GAPDH
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 170 |
| Experiment ID |
547 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 171 |
| Experiment ID |
548 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 172 |
| Experiment ID |
549 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 173 |
| Experiment ID |
550 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 174 |
| Experiment ID |
551 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 175 |
| Experiment ID |
554 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 176 |
| Experiment ID |
555 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 177 |
| Experiment ID |
556 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 178 |
| Experiment ID |
557 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975-fraction 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 179 |
| Experiment ID |
558 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975-fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 180 |
| Experiment ID |
559 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975-fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 181 |
| Experiment ID |
560 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 182 |
| Experiment ID |
561 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 183 |
| Experiment ID |
562 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 184 |
| Experiment ID |
563 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 185 |
| Experiment ID |
564 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 186 |
| Experiment ID |
565 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 187 |
| Experiment ID |
566 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 188 |
| Experiment ID |
567 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 189 |
| Experiment ID |
568 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 190 |
| Experiment ID |
986 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 191 |
| Experiment ID |
987 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H358 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 192 |
| Experiment ID |
988 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|TSG101|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H1264 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 193 |
| Experiment ID |
991 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 194 |
| Experiment ID |
992 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H358 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 195 |
| Experiment ID |
993 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30089911
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response |
| Authors |
"Chen G, Huang AC, Zhang W, Zhang G, Wu M, Xu W, Yu Z, Yang J, Wang B, Sun H, Xia H, Man Q, Zhong W, Antelo LF, Wu B, Xiong X, Liu X, Guan L, Li T, Liu S, Yang R, Lu Y, Dong L, McGettigan S, Somasundaram R, Radhakrishnan R, Mills G, Lu Y, Kim J, Chen YH, Dong H, Zhao Y, Karakousis GC, Mitchell TC, Schuchter LM, Herlyn M, Wherry EJ, Xu X, Guo W." |
| Journal name |
Nature
|
| Publication year |
2018 |
| Sample |
Lung cancer cells |
| Sample name |
H1264 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 196 |
| Experiment ID |
1067 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 197 |
| Experiment ID |
1068 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 198 |
| Experiment ID |
1069 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 199 |
| Experiment ID |
1070 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 200 |
| Experiment ID |
1071 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 201 |
| Experiment ID |
1072 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 202 |
| Experiment ID |
1073 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 203 |
| Experiment ID |
1074 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 204 |
| Experiment ID |
1075 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 205 |
| Experiment ID |
1076 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD63|GAPDH
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34500436
|
| Organism |
Homo sapiens |
| Experiment description |
"MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells" |
| Authors |
"Wen H, Liu Z, Tang J, Bu L." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H2170 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 206 |
| Experiment ID |
1084 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34497265
|
| Organism |
Homo sapiens |
| Experiment description |
"Tumor-derived exosomal miR-3157-3p promotes angiogenesis, vascular permeability and metastasis by targeting TIMP/KLF2 in non-small cell lung cancer" |
| Authors |
"Ma Z, Wei K, Yang F, Guo Z, Pan C, He Y, Wang J, Li Z, Chen L, Chen Y, Xia Y." |
| Journal name |
Cell Death Dis
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 207 |
| Experiment ID |
1085 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34497265
|
| Organism |
Homo sapiens |
| Experiment description |
"Tumor-derived exosomal miR-3157-3p promotes angiogenesis, vascular permeability and metastasis by targeting TIMP/KLF2 in non-small cell lung cancer" |
| Authors |
"Ma Z, Wei K, Yang F, Guo Z, Pan C, He Y, Wang J, Li Z, Chen L, Chen Y, Xia Y." |
| Journal name |
Cell Death Dis
|
| Publication year |
2021 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 208 |
| Experiment ID |
1219 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
31792209
|
| Organism |
Homo sapiens |
| Experiment description |
Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles |
| Authors |
"Xie X, Nie H, Zhou Y, Lian S, Mei H, Lu Y, Dong H, Li F, Li T, Li B, Wang J, Lin M, Wang C, Shao J, Gao Y, Chen J, Xie F, Jia L." |
| Journal name |
Nat Commun
| | | | |