Gene ontology annotations for CD63
Experiment description of studies that identified CD63 in exosomes
1
Experiment ID
11
ISEV standards
✘
EV Biophysical techniques
✔
HSC70|HSP90
EV Cytosolic markers
✔
MHCII|CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
12519789
Organism
Homo sapiens
Experiment description
Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation.
Authors
Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W
Journal name
JBC
Publication year
2003
Sample
B cells
Sample name
RN (HLA-DR15+)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting Thin layer chromatography HPLC
2
Experiment ID
16
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
9685355
Organism
Homo sapiens
Experiment description
Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes.
Authors
Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ
Journal name
JBC
Publication year
1998
Sample
B cells
Sample name
RN (HLA-DR15+)
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.12-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Immunoelectron Microscopy
3
Experiment ID
47
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|CD86
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
17641064
Organism
Homo sapiens
Experiment description
Exosomes with immune modulatory features are present in human breast milk.
Authors
Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name
JIMMU
Publication year
2007
Sample
B cells
Sample name
B cells
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
4
Experiment ID
116
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
B cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
5
Experiment ID
76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Bladder cancer cells
Sample name
HT1376
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS
6
Experiment ID
42
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Bone marrow cells
Sample name
PBMC
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
7
Experiment ID
46
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|MHCII|CD81|CD86
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy FACS
PubMed ID
17641064
Organism
Homo sapiens
Experiment description
Exosomes with immune modulatory features are present in human breast milk.
Authors
Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name
JIMMU
Publication year
2007
Sample
Breast milk
Sample name
Breast milk - Colostrum
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.10-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS
8
Experiment ID
48
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|CD86|MHCII
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy FACS
PubMed ID
17641064
Organism
Homo sapiens
Experiment description
Exosomes with immune modulatory features are present in human breast milk.
Authors
Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S
Journal name
JIMMU
Publication year
2007
Sample
Breast milk
Sample name
Breast milk - Mature milk
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.10-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QSTAR] Western blotting Immunoelectron Microscopy FACS
9
Experiment ID
124
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron microscopy
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Breast milk
Sample name
Breast milk - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
10
Experiment ID
49
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD86
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Immunoelectron Microscopy
PubMed ID
14582906
Organism
Homo sapiens
Experiment description
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors
Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name
ERJ
Publication year
2003
Sample
Bronchoalveolar lavage fluid
Sample name
Bronchoalveolar lavage fluid
Isolation/purification methods
Differential centrifugation Immunobeads (EpCAM)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy
11
Experiment ID
20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
12
Experiment ID
22
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Immunoelectron Microscopy FACS
PubMed ID
15940614
Organism
Homo sapiens
Experiment description
Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape.
Authors
Huber V, Fais S, Iero M, Lugini L, Canese P, Squarcina P, Zaccheddu A, Colone M, Arancia G, Gentile M, Seregni E, Valenti R, Ballabio G, Belli F, Leo E, Parmiani G, Rivoltini L.
Journal name
GASTRO
Publication year
2005
Sample
Colorectal cancer cells
Sample name
SW403 1869col CRC28462
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Immunoelectron Microscopy FACS
13
Experiment ID
201
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22740476
Organism
Homo sapiens
Experiment description
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors
Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ.
Journal name
Electrophoresis
Publication year
2012
Sample
Colorectal cancer cells
Sample name
SW480
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
14
Experiment ID
207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DKO-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
15
Experiment ID
208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
Dks-8
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
16
Experiment ID
209
ISEV standards
✘
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23161513
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name
Mol Cell Proteomics
Publication year
2012
Sample
Colorectal cancer cells
Sample name
DLD-1
Isolation/purification methods
Differential centrifugation Filtration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
17
Experiment ID
285
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
18
Experiment ID
286
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
19
Experiment ID
50
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD87
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Immunoelectron Microscopy
PubMed ID
14582906
Organism
Homo sapiens
Experiment description
Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
Authors
Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name
ERJ
Publication year
2003
Sample
Dendritic cells
Sample name
Monocyte-derived dendritic cells
Isolation/purification methods
Differential centrifugation Immunobeads (EpCAM)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy
20
Experiment ID
117
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
Dendritic cells
Sample name
Dendritic cells
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
21
Experiment ID
272
ISEV standards
✔
AFM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25498500
Organism
Homo sapiens
Experiment description
Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components.
Authors
Toda Y, Takata K, Nakagawa Y, Kawakami H, Fujioka S, Kobayashi K, Hattori Y, Kitamura Y, Akaji K, Ashihara E.
Journal name
Biochem Biophys Res Commun
Publication year
2015
Sample
Glioblastoma cells
Sample name
U251
Isolation/purification methods
Differential centrifugation Ultracenrifugation Sucrose density gradient
Flotation density
1.16 g/mL
Molecules identified in the study
Lipids Protein
Methods used in the study
Thin layer chromatography Western blotting
22
Experiment ID
234
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-C3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
23
Experiment ID
4
ISEV standards
✔
IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|MHCI|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
11487543
Organism
Homo sapiens
Experiment description
Intestinal epithelial cells secrete exosome-like vesicles.
Authors
van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M
Journal name
Publication year
2001
Sample
Intestinal epithelial cells
Sample name
HT29-19A T84-DRB1*0401/CIITA
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
24
Experiment ID
41
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|MHCI|CD9|CD81|TFRC|LAMP2
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy FACS Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Mast cells
Sample name
HMC-1
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS Immunoelectron Microscopy
25
Experiment ID
74
ISEV standards
✘
EV Biophysical techniques
✔
RAB5B
EV Cytosolic markers
✔
LAMP1|CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting FACS ELISA
PubMed ID
19381331
Organism
Homo sapiens
Experiment description
High levels of exosomes expressing CD63 and caveolin-1 in plasma of melanoma patients.
Authors
Logozzi M, De Milito A, Lugini L, Borghi M, Calabrò L, Spada M, Perdicchio M, Marino ML, Federici C, Iessi E, Brambilla D, Venturi G, Lozupone F, Santinami M, Huber V, Maio M, Rivoltini L, Fais S
Journal name
PLoS One
Publication year
2009
Sample
Melanoma cells
Sample name
Me501
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting ELISA FACS
26
Experiment ID
254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
MNT-1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
27
Experiment ID
255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
G1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
28
Experiment ID
256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
501mel
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
29
Experiment ID
257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
Daju
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
30
Experiment ID
258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
31
Experiment ID
259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
32
Experiment ID
260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
1205Lu
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
33
Experiment ID
126
ISEV standards
✘
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra] Western blotting
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
34
Experiment ID
261
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD81|LAMP2|CD9
EV Membrane markers
✘
EV Negative markers
✔
Y
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron microscopy
PubMed ID
25669974
Organism
Homo sapiens
Experiment description
Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins,and metabolites
Authors
Krishna C. Vallabhaneni1, Patrice Penfornis, Santosh Dhule, Francois Guillonneau, Kristen V. Adams, Yin Yuan Mo, Rui Xu, Yiming Liu, Kounosuke Watabe, Mohan C. Vemuri and Radhika Pochampally
Journal name
Oncotarget
Publication year
2014
Sample
Mesenchymal stem cells
Sample name
hMSCs
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA Lipids Metabolites
Methods used in the study
Western blotting Mass spectrometry RT-PCR Sequencing
35
Experiment ID
25
ISEV standards
✔
IEM
EV Biophysical techniques
✔
HSP90|beta-tubulin
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy
PubMed ID
15111327
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes secreted by human mesothelioma cells.
Authors
Hegmans JP, Bard MP, Hemmes A, Luider TM, Kleijmeer MJ, Prins JB, Zitvogel L, Burgers SA, Hoogsteden HC, Lambrecht BN.
Journal name
AJP
Publication year
2004
Sample
Mesothelioma cells
Sample name
PMR-MM7 PMR-MM8
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
36
Experiment ID
219
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
C666-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
37
Experiment ID
220
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP69
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
38
Experiment ID
221
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857718
Organism
Homo sapiens
Experiment description
Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors
Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name
Int J Cancer
Publication year
2015
Sample
Nasopharyngeal carcinoma cells
Sample name
NP460
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.17-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
39
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
40
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
41
Experiment ID
103
ISEV standards
✘
EV Biophysical techniques
✔
Alix|SDCBP
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
21763319
Organism
Homo sapiens
Experiment description
Characterization of soluble and exosomal forms of the EGFR released from pancreatic cancer cells.
Authors
Adamczyk KA, Klein-Scory S, Tehrani MM, Warnken U, Schmiegel W, Schnolzer M, Schwarte-Waldhoff I.
Journal name
LS
Publication year
2011
Sample
Pancreatic cancer cells
Sample name
Panc1
Isolation/purification methods
Differential centrifugation Filtration Ultrafiltration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
42
Experiment ID
40
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9|MHCII|TFRC|LAMP2|ITGA2B
EV Membrane markers
✔
CANX
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy FACS Western blotting
PubMed ID
15908444
Organism
Homo sapiens
Experiment description
Exosomal-like vesicles are present in human blood plasma.
Authors
Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C
Journal name
INTIMM
Publication year
2005
Sample
Plasma
Sample name
Plasma - Normal
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.15-1.27 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS Immunoelectron Microscopy
43
Experiment ID
75
ISEV standards
✘
EV Biophysical techniques
✔
RAB5B
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting FACS ELISA
PubMed ID
19381331
Organism
Homo sapiens
Experiment description
High levels of exosomes expressing CD63 and caveolin-1 in plasma of melanoma patients.
Authors
Logozzi M, De Milito A, Lugini L, Borghi M, Calabrò L, Spada M, Perdicchio M, Marino ML, Federici C, Iessi E, Brambilla D, Venturi G, Lozupone F, Santinami M, Huber V, Maio M, Rivoltini L, Fais S
Journal name
PLoS One
Publication year
2009
Sample
Plasma
Sample name
Plasma - Melanoma
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting ELISA FACS
44
Experiment ID
123
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron microscopy
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Plasma
Sample name
Plasma - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
45
Experiment ID
31
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS Immunoelectron Microscopy
PubMed ID
10572093
Organism
Homo sapiens
Experiment description
Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules.
Authors
Heijnen HF, Schiel AE, Fijnheer R, Geuze HJ, Sixma JJ
Journal name
BLOOD
Publication year
1999
Sample
Platelets
Sample name
Platelets
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.14-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting FACS Immunoelectron Microscopy
46
Experiment ID
231
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry Western blotting
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
47
Experiment ID
232
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
48
Experiment ID
233
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
49
Experiment ID
245
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD9|CD63|FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25519911
Organism
Homo sapiens
Experiment description
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors
Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A.
Journal name
J Biol Chem
Publication year
2015
Sample
Prostate cancer cells
Sample name
PC-3 - Ethanol treated (control)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids Protein
Methods used in the study
Western blotting Mass spectrometry
50
Experiment ID
246
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD9|CD63|FLOT1
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25519911
Organism
Homo sapiens
Experiment description
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors
Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A.
Journal name
J Biol Chem
Publication year
2015
Sample
Prostate cancer cells
Sample name
PC-3 - sn-1-O-hexadecylglycerol treated
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Lipids
Methods used in the study
Western blotting Mass spectrometry
51
Experiment ID
250
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
25857301
Organism
Homo sapiens
Experiment description
Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors
Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - expressing DIAPH3 hRNA
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
52
Experiment ID
275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Flow cytometric analysis Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
53
Experiment ID
274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting Flow cytometric analysis Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
54
Experiment ID
66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
19199708
Organism
Homo sapiens
Experiment description
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name
JPR
Publication year
2009
Sample
Saliva
Sample name
Saliva
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
55
Experiment ID
122
ISEV standards
✔
IEM|EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD9|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron microscopy
PubMed ID
21235781
Organism
Homo sapiens
Experiment description
Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages.
Authors
Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H.
Journal name
JTM
Publication year
2011
Sample
Saliva
Sample name
Saliva - Normal
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS Immunoelectron Microscopy Western blotting
56
Experiment ID
88
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunoelectron Microscopy
PubMed ID
20949044
Organism
Homo sapiens
Experiment description
Let-7 MicroRNA Family Is Selectively Secreted into the Extracellular Environment via Exosomes in a Metastatic Gastric Cancer Cell Line
Authors
Ohshima K, Inoue K, Fujiwara A, Hatakeyama K, Kanto K, Watanabe Y, Muramatsu K, Fukuda Y, Ogura S, Yamaguchi K, Mochizuki T.
Journal name
PLoS One
Publication year
2010
Sample
Stomach cancer cells
Sample name
AZ-P7a
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Immunoelectron Microscopy Western blotting RT-PCR Human miRNA Microarray Kit
57
Experiment ID
18
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
11907077
Organism
Homo sapiens
Experiment description
TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors
Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name
JIMMU
Publication year
2002
Sample
T cells
Sample name
Jurkat T cell blasts
Isolation/purification methods
Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
58
Experiment ID
91
ISEV standards
✔
EM
EV Biophysical techniques
✘
EV Cytosolic markers
✔
LAMP1|CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Immunofluorescence Western blotting FACS
PubMed ID
21252909
Organism
Homo sapiens
Experiment description
Diacylglycerol kinase a regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes
Authors
Alonso R, Mazzeo C, Rodriguez MC, Marsh M, Fraile-Ramos A, Calvo V, Avila-Flores A, Merida I, Izquierdo M.
Journal name
CDD
Publication year
2011
Sample
T cells
Sample name
J-HM1-2.2
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting FACS Immunofluorescence
59
Experiment ID
115
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
21505438
Organism
Homo sapiens
Experiment description
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells.
Authors
Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÁ, Bernad A, Sánchez-Madrid F.
Journal name
NATCOM
Publication year
2011
Sample
T cells
Sample name
J77
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Mass spectrometry Microarray
60
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
61
Experiment ID
34
ISEV standards
✔
EM|IEM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD63|MHCI|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting FACS
PubMed ID
19190083
Organism
Homo sapiens
Experiment description
Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors
Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
Journal name
FASEB
Publication year
2009
Sample
Tracheobronchial cells
Sample name
Tracheobronchial epithelial cells
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
1.16 -1.18 g/mL
Molecules identified in the study
Protein mRNA
Methods used in the study
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy FACS
62
Experiment ID
13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
15326289
Organism
Homo sapiens
Experiment description
Identification and proteomic profiling of exosomes in human urine.
Authors
Pisitkun T, Shen RF, Knepper MA
Journal name
PNAS
Publication year
2004
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LCQ DECA XP] Western blotting
63
Experiment ID
63
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
64
Experiment ID
77
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
65
Experiment ID
78
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
FACS
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name
MCP
Publication year
2010
Sample
Urine
Sample name
Urine - Bladder cancer
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
FACS
66
Experiment ID
196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal high density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
67
Experiment ID
197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
22418980
Organism
Homo sapiens
Experiment description
A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors
Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name
Kidney Int
Publication year
2012
Sample
Urine
Sample name
Urine - Normal low density
Isolation/purification methods
Differential centrifugation Sucrose cushion
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for CD63
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ITGB1
3688
Affinity Capture-Western
Homo sapiens
2
CD81
975
Invivo
Homo sapiens
3
HLA-DOB
Affinity Capture-Western
Homo sapiens
4
KIT
3815
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
5
CD9
928
Invivo
Homo sapiens
Affinity Capture-Western
Homo sapiens
6
ITGA6
3655
Invitro
Homo sapiens
7
HLA-DMB
3109
Affinity Capture-Western
Homo sapiens
8
PTGFRN
5738
Affinity Capture-Western
Homo sapiens
9
CD82
3732
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
10
AP4M1
9179
Two-hybrid
Homo sapiens
11
HLA-DRA
3122
Affinity Capture-Western
Homo sapiens
View the network
image/svg+xml
Pathways in which CD63 is involved