Gene description for LPL
Gene name lipoprotein lipase
Gene symbol LPL
Other names/aliases HDLCQ11
LIPD
Species Homo sapiens
 Database cross references - LPL
ExoCarta ExoCarta_4023
Vesiclepedia VP_4023
Entrez Gene 4023
HGNC 6677
MIM 609708
UniProt P06858  
 LPL identified in sEVs derived from the following tissue/cell type
Breast milk 17641064    
Breast milk 17641064    
Chondrocytes 35931686    
Chondrocytes 35931686    
Prostate cancer cells 25844599    
 Gene ontology annotations for LPL
Molecular Function
    lipoprotein lipase activity GO:0004465 IBA
    lipoprotein lipase activity GO:0004465 IDA
    lipoprotein lipase activity GO:0004465 IMP
    lipoprotein lipase activity GO:0004465 ISS
    phospholipase activity GO:0004620 ISS
    triacylglycerol lipase activity GO:0004806 IDA
    triacylglycerol lipase activity GO:0004806 ISS
    signaling receptor binding GO:0005102 IPI
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    heparin binding GO:0008201 IDA
    phospholipase A1 activity GO:0008970 IBA
    phospholipase A1 activity GO:0008970 IDA
    apolipoprotein binding GO:0034185 IBA
    apolipoprotein binding GO:0034185 IPI
    protein homodimerization activity GO:0042803 IDA
    heparan sulfate proteoglycan binding GO:0043395 IMP
    protein-membrane adaptor activity GO:0043495 TAS
    phosphatidylserine 1-acylhydrolase activity GO:0052739 IEA
    lipoprotein particle binding GO:0071813 IDA
Biological Process
    fatty acid metabolic process GO:0006631 IDA
    fatty acid biosynthetic process GO:0006633 IBA
    fatty acid biosynthetic process GO:0006633 IC
    fatty acid biosynthetic process GO:0006633 IDA
    fatty acid biosynthetic process GO:0006633 ISS
    triglyceride metabolic process GO:0006641 ISS
    phospholipid metabolic process GO:0006644 ISS
    response to bacterium GO:0009617 IEA
    response to glucose GO:0009749 ISS
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IC
    positive regulation of lipid storage GO:0010884 TAS
    positive regulation of cholesterol storage GO:0010886 IMP
    positive regulation of sequestering of triglyceride GO:0010890 IMP
    triglyceride catabolic process GO:0019433 IBA
    triglyceride catabolic process GO:0019433 IDA
    triglyceride catabolic process GO:0019433 ISS
    cellular response to nutrient GO:0031670 ISS
    positive regulation of chemokine production GO:0032722 IMP
    positive regulation of interleukin-1 beta production GO:0032731 ISS
    positive regulation of interleukin-6 production GO:0032755 ISS
    positive regulation of tumor necrosis factor production GO:0032760 ISS
    chylomicron remodeling GO:0034371 IC
    chylomicron remodeling GO:0034371 IMP
    very-low-density lipoprotein particle remodeling GO:0034372 IBA
    very-low-density lipoprotein particle remodeling GO:0034372 IDA
    very-low-density lipoprotein particle remodeling GO:0034372 ISS
    high-density lipoprotein particle remodeling GO:0034375 IBA
    cholesterol homeostasis GO:0042632 IBA
    cholesterol homeostasis GO:0042632 IMP
    positive regulation of fat cell differentiation GO:0045600 IMP
    positive regulation of inflammatory response GO:0050729 IC
    positive regulation of inflammatory response GO:0050729 ISS
    low-density lipoprotein particle mediated signaling GO:0055096 IMP
    triglyceride homeostasis GO:0070328 IGI
    triglyceride homeostasis GO:0070328 NAS
    cellular response to fatty acid GO:0071398 ISS
    positive regulation of adipose tissue development GO:1904179 IMP
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IEA
    cell surface GO:0009986 IEA
    very-low-density lipoprotein particle GO:0034361 IEA
    chylomicron GO:0042627 IEA
    catalytic complex GO:1902494 IPI
 Experiment description of studies that identified LPL in sEVs
1
Experiment ID 46
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|MHCII|CD81|CD86
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Colostrum
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
2
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
3
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for LPL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VLDLR 7436
Reconstituted Complex Homo sapiens
2 NEIL3  
Protein-RNA Homo sapiens
3 LRP2 4036
Reconstituted Complex Homo sapiens
4 LPL 4023
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
5 E2F4  
Affinity Capture-MS Homo sapiens
6 COPS6 10980
Two-hybrid Homo sapiens
7 UBE2Z 65264
Two-hybrid Homo sapiens
8 CALR 811
Affinity Capture-Western Homo sapiens
9 CUL3 8452
Affinity Capture-MS Homo sapiens
10 EMD 2010
Two-hybrid Homo sapiens
11 KIAA1377  
Two-hybrid Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
13 LRP1 4035
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 C12orf65  
Affinity Capture-MS Homo sapiens
15 LUC7L2 51631
Two-hybrid Homo sapiens
16 RPL18A 6142
Two-hybrid Homo sapiens
17 EEF2KMT  
Affinity Capture-MS Homo sapiens
18 PLK1 5347
Affinity Capture-MS Homo sapiens
19 PTPN4  
Two-hybrid Homo sapiens
20 ASCC2 84164
Two-hybrid Homo sapiens
21 TSC22D2 9819
Two-hybrid Homo sapiens
22 DCX  
Affinity Capture-MS Homo sapiens
23 CCND1 595
Two-hybrid Homo sapiens
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