Gene description for F8
Gene name coagulation factor VIII, procoagulant component
Gene symbol F8
Other names/aliases AHF
DXS1253E
F8B
F8C
FVIII
HEMA
Species Homo sapiens
 Database cross references - F8
ExoCarta ExoCarta_2157
Vesiclepedia VP_2157
Entrez Gene 2157
HGNC 3546
MIM 300841
UniProt P00451  
 F8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for F8
Molecular Function
    copper ion binding GO:0005507 IEA
    protein binding GO:0005515 IPI
    oxidoreductase activity GO:0016491 IEA
Biological Process
    acute-phase response GO:0006953 IEA
    blood coagulation GO:0007596 TAS
    blood coagulation, intrinsic pathway GO:0007597 IBA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    COPII-coated ER to Golgi transport vesicle GO:0030134 TAS
    platelet alpha granule lumen GO:0031093 TAS
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 TAS
 Experiment description of studies that identified F8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for F8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 F10 2159
Reconstituted Complex Homo sapiens
2 TSC22D2 9819
Two-hybrid Homo sapiens
3 CALR 811
Co-localization Homo sapiens
4 PROS1 5627
Reconstituted Complex Homo sapiens
5 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
6 LRP1 4035
Reconstituted Complex Homo sapiens
7 BRCA2 675
Affinity Capture-MS Homo sapiens
8 UBQLN1 29979
Two-hybrid Homo sapiens
9 VWF 7450
Reconstituted Complex Homo sapiens
10 GGA1 26088
Two-hybrid Homo sapiens
11 CANX 821
Affinity Capture-Western Homo sapiens
12 PHYH 5264
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 LMAN1 3998
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which F8 is involved
PathwayEvidenceSource
Asparagine N-linked glycosylation TAS Reactome
Cargo concentration in the ER TAS Reactome
Common Pathway of Fibrin Clot Formation TAS Reactome
COPII-mediated vesicle transport TAS Reactome
Defective cofactor function of FVIIIa variant TAS Reactome
Defective F8 accelerates dissociation of the A2 domain TAS Reactome
Defective F8 binding to the cell membrane TAS Reactome
Defective F8 binding to von Willebrand factor TAS Reactome
Defective F8 cleavage by thrombin TAS Reactome
Defective F8 secretion TAS Reactome
Defective F8 sulfation at Y1699 TAS Reactome
Defective F9 variant does not activate FX TAS Reactome
Defective factor IX causes hemophilia B TAS Reactome
Defective factor IX causes thrombophilia TAS Reactome
Defective factor VIII causes hemophilia A TAS Reactome
Defects of contact activation system (CAS) and kallikrein/kinin system (KKS) TAS Reactome
Disease TAS Reactome
Diseases of hemostasis TAS Reactome
ER to Golgi Anterograde Transport TAS Reactome
Formation of Fibrin Clot (Clotting Cascade) TAS Reactome
Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation TAS Reactome
Hemostasis TAS Reactome
Intrinsic Pathway of Fibrin Clot Formation TAS Reactome
Membrane Trafficking TAS Reactome
Metabolism of proteins TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet degranulation TAS Reactome
Post-translational protein modification TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
Transport to the Golgi and subsequent modification TAS Reactome
Vesicle-mediated transport TAS Reactome





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