Gene description for TNKS2
Gene name tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
Gene symbol TNKS2
Other names/aliases ARTD6
PARP-5b
PARP-5c
PARP5B
PARP5C
TANK2
TNKL
pART6
Species Homo sapiens
 Database cross references - TNKS2
ExoCarta ExoCarta_80351
Entrez Gene 80351
HGNC 15677
MIM 607128
UniProt Q9H2K2  
 TNKS2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for TNKS2
Molecular Function
    enzyme binding GO:0019899 IPI
    NAD+ ADP-ribosyltransferase activity GO:0003950 TAS
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
Biological Process
    regulation of multicellular organism growth GO:0040014 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    protein auto-ADP-ribosylation GO:0070213 IDA
    Wnt signaling pathway GO:0016055 IEA
    protein polyubiquitination GO:0000209 IDA
    positive regulation of telomere maintenance via telomerase GO:0032212 IC
    protein localization to chromosome, telomeric region GO:0070198 IMP
    multicellular organism growth GO:0035264 IEA
    protein ADP-ribosylation GO:0006471 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nuclear envelope GO:0005635 IDA
    pericentriolar material GO:0000242 IDA
    cytoplasm GO:0005737 IDA
    Golgi membrane GO:0000139 IEA
    nuclear chromosome, telomeric region GO:0000784 IC
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified TNKS2 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for TNKS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Biochemical Activity Homo sapiens
2 TNKS1BP1 85456
Two-hybrid Homo sapiens
3 TERF1 7013
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Biochemical Activity Homo sapiens
4 LNPEP 4012
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
5 GRB14  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 TNKS 8658
Invivo Homo sapiens
View the network image/svg+xml



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