Gene description for IDH3A
Gene name isocitrate dehydrogenase 3 (NAD+) alpha
Gene symbol IDH3A
Other names/aliases -
Species Homo sapiens
 Database cross references - IDH3A
ExoCarta ExoCarta_3419
Entrez Gene 3419
HGNC 5384
MIM 601149
UniProt P50213  
 IDH3A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for IDH3A
Molecular Function
    magnesium ion binding GO:0000287 IEA
    isocitrate dehydrogenase (NAD+) activity GO:0004449 IEA
    NAD binding GO:0051287 IEA
Biological Process
    cellular metabolic process GO:0044237 TAS
    small molecule metabolic process GO:0044281 TAS
    tricarboxylic acid cycle GO:0006099 TAS
    carbohydrate metabolic process GO:0005975 NAS
Subcellular Localization
    mitochondrial matrix GO:0005759 TAS
    mitochondrion GO:0005739 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified IDH3A in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IDH3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IDH3G  
Invivo Homo sapiens
2 IDH3B 3420
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which IDH3A is involved
PathwayEvidenceSource
Citric acid cycle (TCA cycle) TAS Reactome





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