Gene description for IRS2
Gene name insulin receptor substrate 2
Gene symbol IRS2
Other names/aliases IRS-2
Species Homo sapiens
 Database cross references - IRS2
ExoCarta ExoCarta_8660
Entrez Gene 8660
HGNC 6126
MIM 600797
UniProt Q9Y4H2  
 IRS2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Neuroblastoma cells 25944692    
 Gene ontology annotations for IRS2
Molecular Function
    protein domain specific binding GO:0019904 IEA
    protein binding GO:0005515 IPI
    phosphatidylinositol 3-kinase binding GO:0043548 IBA
    14-3-3 protein binding GO:0071889 IEA
    signal transducer activity GO:0004871 IEA
    protein kinase binding GO:0019901 IEA
    insulin receptor binding GO:0005158 IBA
    protein phosphatase binding GO:0019903 IEA
Biological Process
    innate immune response GO:0045087 TAS
    brain development GO:0007420 IEA
    positive regulation of glucose import GO:0046326 IMP
    response to glucose GO:0009749 ISS
    mammary gland development GO:0030879 IEA
    positive regulation of cell migration GO:0030335 IEA
    signal transduction GO:0007165 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    negative regulation of B cell apoptotic process GO:0002903 ISS
    insulin receptor signaling pathway GO:0008286 TAS
    positive regulation of insulin secretion GO:0032024 ISS
    lipid homeostasis GO:0055088 TAS
    positive regulation of B cell proliferation GO:0030890 ISS
    glucose metabolic process GO:0006006 TAS
    cell proliferation GO:0008283 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    cellular response to glucose stimulus GO:0071333 IEA
    positive regulation of glucose metabolic process GO:0010907 IMP
    cellular response to insulin stimulus GO:0032869 IMP
    negative regulation of kinase activity GO:0033673 ISS
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    positive regulation of cell proliferation GO:0008284 NAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    negative regulation of plasma membrane long-chain fatty acid transport GO:0010748 IMP
    regulation of lipid metabolic process GO:0019216 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of fatty acid beta-oxidation GO:0032000 IMP
    positive regulation of glycogen biosynthetic process GO:0045725 NAS
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified IRS2 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for IRS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAE 7531
Invivo Homo sapiens
2 YWHAQ 10971
Affinity Capture-MS Homo sapiens
3 IL4R  
Invitro Homo sapiens
4 UBTF 7343
Invivo Homo sapiens
5 BCL2  
Affinity Capture-Western Homo sapiens
6 PIK3R1 5295
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 PTPN11 5781
Affinity Capture-Western Homo sapiens
8 YWHAG 7532
Affinity Capture-MS Homo sapiens
9 INSR 3643
Two-hybrid Homo sapiens
10 YWHAZ 7534
Invivo Homo sapiens
11 SOCS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 PLCG1 5335
Invivo Homo sapiens
13 SFN 2810
Affinity Capture-MS Homo sapiens
14 GRB2 2885
Invivo Homo sapiens
15 TYK2 7297
Invivo Homo sapiens
Invitro Homo sapiens
16 EPOR  
Affinity Capture-Western Homo sapiens
17 PIK3R3  
Affinity Capture-Western Homo sapiens
18 JAK1 3716
Affinity Capture-Western Homo sapiens
19 YWHAB 7529
Affinity Capture-MS Homo sapiens
20 SHC1 6464
Invitro Homo sapiens
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