Gene description for FLG2
Gene name filaggrin family member 2
Gene symbol FLG2
Other names/aliases IFPS
Species Homo sapiens
 Database cross references - FLG2
ExoCarta ExoCarta_388698
Vesiclepedia VP_388698
Entrez Gene 388698
HGNC 33276
MIM 616284
UniProt Q5D862  
 FLG2 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for FLG2
Molecular Function
    structural molecule activity GO:0005198 IEA
    calcium ion binding GO:0005509 IEA
    transition metal ion binding GO:0046914 IEA
Biological Process
    cell adhesion GO:0007155 IMP
    epidermis morphogenesis GO:0048730 IMP
    establishment of skin barrier GO:0061436 IBA
    establishment of skin barrier GO:0061436 IEP
Subcellular Localization
    cornified envelope GO:0001533 IBA
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 IDA
    keratohyalin granule GO:0036457 IBA
    tertiary granule lumen GO:1904724 TAS
 Experiment description of studies that identified FLG2 in exosomes
1
Experiment ID 494
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for FLG2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRSS16 10279
Affinity Capture-MS Homo sapiens
2 CDC73  
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 TP53 7157
Affinity Capture-MS Homo sapiens
5 CASP14 23581
Affinity Capture-MS Homo sapiens
6 MYCN  
Affinity Capture-MS Homo sapiens
7 PARP1 142
Affinity Capture-MS Homo sapiens
8 MAD2L1 4085
Affinity Capture-MS Homo sapiens
9 MAGEA3  
Affinity Capture-MS Homo sapiens
10 GMCL1  
Affinity Capture-MS Homo sapiens
11 METTL14  
Affinity Capture-MS Homo sapiens
12 CCNC  
Affinity Capture-MS Homo sapiens
13 PTDSS1 9791
Affinity Capture-MS Homo sapiens
14 RPS19BP1  
Affinity Capture-MS Homo sapiens
15 Fcho2  
Affinity Capture-MS Mus musculus
16 TBC1D22B  
Affinity Capture-MS Homo sapiens
17 BTF3 689
Affinity Capture-MS Homo sapiens
18 IQCB1  
Affinity Capture-MS Homo sapiens
19 HRNR 388697
Affinity Capture-MS Homo sapiens
20 JUP 3728
Affinity Capture-MS Homo sapiens
21 CD274 29126
Affinity Capture-MS Homo sapiens
22 RLIM 51132
Affinity Capture-MS Homo sapiens
23 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
24 Ckap5 75786
Affinity Capture-MS Mus musculus
25 DSC1 1823
Affinity Capture-MS Homo sapiens
26 PI4KAP1  
Affinity Capture-MS Homo sapiens
27 WTAP 9589
Affinity Capture-MS Homo sapiens
28 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
29 FTH1 2495
Affinity Capture-MS Homo sapiens
30 PKP1 5317
Affinity Capture-MS Homo sapiens
31 CDK1 983
Affinity Capture-MS Homo sapiens
32 SH2D3A  
Affinity Capture-MS Homo sapiens
33 SERPINB12 89777
Affinity Capture-MS Homo sapiens
34 MECP2 4204
Affinity Capture-MS Homo sapiens
35 ESR1  
Affinity Capture-MS Homo sapiens
36 STX17 55014
Affinity Capture-MS Homo sapiens
37 UFL1 23376
Affinity Capture-MS Homo sapiens
38 KIAA1429 25962
Affinity Capture-MS Homo sapiens
39 FASTKD3  
Proximity Label-MS Homo sapiens
40 CDK2 1017
Affinity Capture-MS Homo sapiens
41 ARG1 383
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 SOAT1 6646
Affinity Capture-MS Homo sapiens
44 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
45 FBXO6 26270
Affinity Capture-MS Homo sapiens
46 TGM3 7053
Affinity Capture-MS Homo sapiens
47 JAK1 3716
Affinity Capture-MS Homo sapiens
48 TRIM21 6737
Affinity Capture-MS Homo sapiens
49 KIF23 9493
Affinity Capture-MS Homo sapiens
50 KLF5  
Affinity Capture-MS Homo sapiens
51 PINK1  
Affinity Capture-MS Homo sapiens
52 Ddb1 13194
Affinity Capture-MS Mus musculus
53 TCF3  
Affinity Capture-MS Homo sapiens
54 METTL3  
Affinity Capture-MS Homo sapiens
55 COPS5 10987
Affinity Capture-MS Homo sapiens
56 ZUFSP 221302
Affinity Capture-MS Homo sapiens
57 DHFRL1  
Proximity Label-MS Homo sapiens
58 VDAC1 7416
Affinity Capture-MS Homo sapiens
59 ARF5 381
Proximity Label-MS Homo sapiens
60 ANXA2 302
Affinity Capture-MS Homo sapiens
61 CUL2 8453
Affinity Capture-MS Homo sapiens
62 DDX3X 1654
Affinity Capture-MS Homo sapiens
63 DDX39B 7919
Affinity Capture-MS Homo sapiens
64 C1orf68 100129271
Affinity Capture-MS Homo sapiens
65 FLG 2312
Affinity Capture-MS Homo sapiens
66 CYLD  
Affinity Capture-MS Homo sapiens
67 CUL4A 8451
Affinity Capture-MS Homo sapiens
68 KPRP 448834
Affinity Capture-MS Homo sapiens
69 FGFRL1 53834
Affinity Capture-MS Homo sapiens
70 DSP 1832
Affinity Capture-MS Homo sapiens
71 DSG1 1828
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FLG2 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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