Gene description for Eps15
Gene name epidermal growth factor receptor pathway substrate 15
Gene symbol Eps15
Other names/aliases 2410112D09Rik
Species Mus musculus
 Database cross references - Eps15
ExoCarta ExoCarta_13858
Vesiclepedia VP_13858
Entrez Gene 13858
UniProt P42567  
 Eps15 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eps15
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    polyubiquitin modification-dependent protein binding GO:0031593 ISO
    polyubiquitin modification-dependent protein binding GO:0031593 ISS
    identical protein binding GO:0042802 IPI
    ubiquitin binding GO:0043130 IDA
    ubiquitin binding GO:0043130 IMP
Biological Process
    positive regulation of receptor recycling GO:0001921 ISO
    Golgi to endosome transport GO:0006895 ISO
    Golgi to endosome transport GO:0006895 ISS
    endocytosis GO:0006897 IBA
    endosomal transport GO:0016197 IBA
    receptor-mediated endocytosis of virus by host cell GO:0019065 ISO
    endocytic recycling GO:0032456 ISO
    regulation of protein localization GO:0032880 IMP
    regulation of cell population proliferation GO:0042127 ISO
    symbiont entry into host cell GO:0046718 ISO
    clathrin coat assembly GO:0048268 ISO
    ubiquitin-dependent endocytosis GO:0070086 IMP
    postsynaptic neurotransmitter receptor internalization GO:0098884 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    clathrin-coated pit GO:0005905 IDA
    basal plasma membrane GO:0009925 ISO
    aggresome GO:0016235 ISO
    apical plasma membrane GO:0016324 ISO
    AP-2 adaptor complex GO:0030122 IDA
    clathrin coat of coated pit GO:0030132 IBA
    clathrin coat of coated pit GO:0030132 IDA
    clathrin-coated vesicle GO:0030136 IDA
    early endosome membrane GO:0031901 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    synapse GO:0045202 ISO
    ciliary membrane GO:0060170 IDA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Eps15 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eps15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ap2m1 11773
Co-fractionation Mus musculus
2 Necap1  
Co-fractionation Mus musculus
3 Fxr1 14359
Affinity Capture-MS Mus musculus
4 Ubc  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
5 Agfg1  
Affinity Capture-Western Mus musculus
6 Ap1b1 11764
Co-fractionation Mus musculus
7 DNM1 1759
Reconstituted Complex Homo sapiens
8 Gja1 14609
Affinity Capture-Western Mus musculus
9 AGFG2  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
10 Ube2d3  
Co-fractionation Mus musculus
11 Amph  
Co-fractionation Mus musculus
12 Vsig8  
Co-fractionation Mus musculus
13 Ap2b1 71770
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
14 Dpysl2 12934
Affinity Capture-Western Mus musculus
15 Krt33a  
Co-fractionation Mus musculus
16 Numb  
Affinity Capture-Western Mus musculus
17 NUMBL 9253
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
18 AGFG1 3267
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
19 CDC40  
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
20 PARK2  
Affinity Capture-Western Homo sapiens
21 Eps15l1  
Co-fractionation Mus musculus
22 EPN2 22905
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
23 Snap91  
Co-fractionation Mus musculus
24 Krt73  
Co-fractionation Mus musculus
25 Spg20  
Co-fractionation Mus musculus
26 Krt36  
Co-fractionation Mus musculus
27 Krt4 16682
Co-fractionation Mus musculus
28 Itsn1 16443
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
29 MSR1  
Affinity Capture-Western Homo sapiens
30 Atg5  
Affinity Capture-RNA Mus musculus
31 NUMB 8650
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
32 Reps2  
Co-fractionation Mus musculus
33 Krt16 16666
Co-fractionation Mus musculus
34 Ap2a2 11772
Co-fractionation Mus musculus
35 Ap2s1 232910
Co-fractionation Mus musculus
36 Necap2  
Co-fractionation Mus musculus
37 AP2A1 160
Protein-peptide Homo sapiens
38 Krt5 110308
Co-fractionation Mus musculus
39 Krt9  
Co-fractionation Mus musculus
40 Ap2a1 11771
Co-fractionation Mus musculus
41 Eps15 13858
Reconstituted Complex Mus musculus
Co-fractionation Mus musculus
Reconstituted Complex Mus musculus
Co-fractionation Mus musculus
42 Krt1 16678
Co-fractionation Mus musculus
43 Krt14 16664
Co-fractionation Mus musculus
44 Rabl6  
Co-fractionation Mus musculus
45 Egfr 13649
Affinity Capture-Western Mus musculus
46 Agfg2 231801
Co-fractionation Mus musculus
47 Epn1  
Co-fractionation Mus musculus
48 Park2  
Reconstituted Complex Mus musculus
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