Gene description for PLCD1
Gene name phospholipase C, delta 1
Gene symbol PLCD1
Other names/aliases NDNC3
PLC-III
Species Homo sapiens
 Database cross references - PLCD1
ExoCarta ExoCarta_5333
Entrez Gene 5333
HGNC 9060
MIM 602142
UniProt P51178  
 PLCD1 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PLCD1
Molecular Function
    calcium ion binding GO:0005509 IEA
    GTPase activating protein binding GO:0032794 IPI
    phosphatidylserine binding GO:0001786 IEA
    signal transducer activity GO:0004871 IEA
    phosphatidylinositol phospholipase C activity GO:0004435 IEA
    phosphatidic acid binding GO:0070300 IEA
    phosphatidylinositol phosphate binding GO:1901981 IEA
Biological Process
    intracellular signal transduction GO:0035556 IEA
    phospholipid metabolic process GO:0006644 TAS
    labyrinthine layer blood vessel development GO:0060716 IEA
    small molecule metabolic process GO:0044281 TAS
    inositol phosphate metabolic process GO:0043647 TAS
    regulation of cell proliferation GO:0042127 IEA
    angiogenesis GO:0001525 IEA
    lipid catabolic process GO:0016042 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified PLCD1 in exosomes
1
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLCD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GAP43  
Affinity Capture-Western Homo sapiens
2 TGM2 7052
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PLCD1 is involved
PathwayEvidenceSource
Synthesis of IP3 and IP4 in the cytosol TAS Reactome





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